<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2016-11-01</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0000949721</issuerCik>
        <issuerName>Meridian Waste Solutions, Inc.</issuerName>
        <issuerTradingSymbol>MRDN</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001689583</rptOwnerCik>
            <rptOwnerName>Ardagna Joseph Francis</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>5055 HEATHERWOOD CR</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>ROSWELL</rptOwnerCity>
            <rptOwnerState>GA</rptOwnerState>
            <rptOwnerZipCode>30075</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock, $0.025 par value per share</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1000</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>NonEmployee Stock Option</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>20</value>
                <footnoteId id="F2"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F2"/>
            </exerciseDate>
            <expirationDate>
                <value>2021-11-01</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock, par value $0.025 per share</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>3750</value>
                    <footnoteId id="F2"/>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Reflects 1 - for - 20 reverse stock split effected by the Company on November 3, 2016.</footnote>
        <footnote id="F2">In connection with his appointment to the Board, Mr. Ardagna received, pursuant to a stock option agreement entered into as of November 1, 2016, by and between the Company and Mr. Ardagna, stock options to purchase up to three thousand seven hundred fifty (3,750) shares of the Company?s common stock, par value $0.025 per share, at an exercise price per share equal to $20.00, giving effect to the Company's 1for20 reverse stock split. The options are exercisable for a period of five (5) years and vest in equal amounts over a period of three (3) years at the rate of three hundred thirteen (313) options per fiscal quarter at the end of such quarter, commencing in the quarter ended December 31, 2016, and prorated for the number of days Mr. Ardagna served on Board during the first fiscal quarter.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Joseph F. Ardagna</signatureName>
        <signatureDate>2016-11-14</signatureDate>
    </ownerSignature>
</ownershipDocument>
