<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-01-31</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0000939930</issuerCik>
        <issuerName>PYXUS INTERNATIONAL, INC.</issuerName>
        <issuerTradingSymbol>PYYX</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0000312069</rptOwnerCik>
            <rptOwnerName>BARCLAYS PLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>1 CHURCHILL PLACE</rptOwnerStreet1>
            <rptOwnerStreet2>CANARY WHARF</rptOwnerStreet2>
            <rptOwnerCity>LONDON</rptOwnerCity>
            <rptOwnerState>X0</rptOwnerState>
            <rptOwnerZipCode>E14 5HP</rptOwnerZipCode>
            <rptOwnerStateDescription>UNITED KINGDOM</rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <remarks>a) The reporting persons did not engage in any reportable transactions during the relevant period. The reporting persons are no longer subject to Section 16 as a result of the unwind of certain stock borrow and repurchase transaction activity that did not create in the reporting persons, a pecuniary interest in the securities.

(b) Name of Person Filing:

   (1) Barclays PLC

   (2) Barclays Bank PLC (controlled by Barclays PLC)

   (3) Barclays Capital Inc. (controlled by Barclays PLC)

(c) Address of Principal Business Office or, if non, Residence:

   (1) Barclays PLC

       1 Churchill Place

       London, E14 5HP, England

   (2) Barclays Bank PLC

       1 Churchill Place

       London, E14 5HP, England

   (3)  Barclays Capital Inc.

       745 Seventh Avenue

       New York, New York 10019

       United States</remarks>

    <ownerSignature>
        <signatureName>Suejean Mott</signatureName>
        <signatureDate>2025-02-04</signatureDate>
    </ownerSignature>
</ownershipDocument>
