<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2025-01-28</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0000939930</issuerCik>
        <issuerName>PYXUS INTERNATIONAL, INC.</issuerName>
        <issuerTradingSymbol>PYYX</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0000312069</rptOwnerCik>
            <rptOwnerName>BARCLAYS PLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>1 CHURCHILL PLACE</rptOwnerStreet1>
            <rptOwnerStreet2>CANARY WHARF</rptOwnerStreet2>
            <rptOwnerCity>LONDON</rptOwnerCity>
            <rptOwnerState>X0</rptOwnerState>
            <rptOwnerZipCode>E14 5HP</rptOwnerZipCode>
            <rptOwnerStateDescription>UNITED KINGDOM</rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common stock, no par value</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>89334</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>Barclays Bank PLC</value>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common stock, no par value</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>3708935</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>Barclays Capital Inc</value>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <remarks>(a) Beneficially owned Common stock, no par value.

(b) Name of Person Filing:

   (1) Barclays PLC

   (2) Barclays Bank PLC (controlled by Barclays PLC)

   (3) Barclays Capital Inc. (controlled by Barclays PLC)

(c) Address of Principal Business Office or, if non, Residence:

   (1) Barclays PLC

       1 Churchill Place

       London, E14 5HP, England

   (2)  Barclays Bank PLC

       1 Churchill Place

       London, E14 5HP, England

  (3)  Barclays Capital Inc.

       745 Seventh Avenue

       New York, New York 10019

       United States</remarks>

    <ownerSignature>
        <signatureName>Suejean Mott</signatureName>
        <signatureDate>2025-01-30</signatureDate>
    </ownerSignature>
</ownershipDocument>
