The Securities and Exchange Commission has not necessarily reviewed the information in this filing and has not determined if it is accurate and complete.
The reader should not assume that the information is accurate and complete.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM 13F

FORM 13F INFORMATION TABLE
OMB APPROVAL
OMB Number: 3235-0006
Estimated average burden
hours per response: 23.8
COLUMN 1 COLUMN 2 COLUMN 3 COLUMN 4 COLUMN 5 COLUMN 6 COLUMN 7 COLUMN 8
VALUE SHRS OR SH/ PUT/ INVESTMENT OTHER VOTING AUTHORITY
NAME OF ISSUER TITLE OF CLASS CUSIP (x$1000) PRN AMT PRN CALL DISCRETION MANAGER SOLE SHARED NONE
AT&T INC COMMON STOCK 00206R102 180 7,627 SH   DFND 1 7,627 0 0
AT&T INC COMMON STOCK 00206R102 24 1,000 SH   OTR 1 1,000 0 0
ABBOTT LABORATORIES COMMON STOCK 002824100 777 6,562 SH   DFND 1 6,562 0 0
ABBVIE INC COMMON STOCK 00287Y109 394 2,428 SH   DFND 1 2,428 0 0
ABBVIE INC COMMON STOCK 00287Y109 28 175 SH   OTR 1 175 0 0
ACTIVISION BLIZZARD INC COMMON STOCK 00507V109 302 3,775 SH   DFND 1 3,775 0 0
ACTIVISION BLIZZARD INC COMMON STOCK 00507V109 10 129 SH   OTR 1 129 0 0
ADOBE INC COMMON STOCK 00724F101 278 610 SH   DFND 1 610 0 0
ADOBE INC COMMON STOCK 00724F101 10 21 SH   OTR 1 21 0 0
AIR TRANSPORT SERVICES GROUP COMMON STOCK 00922R105 312 9,332 SH   DFND 1 9,332 0 0
ALPHABET INC COMMON STOCK 02079K305 1,007 362 SH   DFND 1 362 0 0
ALPHABET INC COMMON STOCK 02079K305 19 7 SH   OTR 1 7 0 0
AMAZON COM INC COMMON STOCK 023135106 1,617 496 SH   DFND 1 496 0 0
AMAZON COM INC COMMON STOCK 023135106 13 4 SH   OTR 1 4 0 0
AMERICAN TOWER CORP COMMON STOCK 03027X100 295 1,175 SH   DFND 1 1,175 0 0
AMGEN INC COMMON STOCK 031162100 200 829 SH   DFND 1 829 0 0
APPLE INC COMMON STOCK 037833100 6,106 34,970 SH   DFND 1 34,970 0 0
APPLE INC COMMON STOCK 037833100 202 1,157 SH   OTR 1 1,157 0 0
APPLIED MATERIALS DELAWARE COMMON STOCK 038222105 612 4,645 SH   DFND 1 4,645 0 0
APPLIED MATERIALS DELAWARE COMMON STOCK 038222105 97 735 SH   OTR 1 735 0 0
AVALON BAY COMMUNITIES INC COMMON STOCK 053484101 264 1,061 SH   DFND 1 1,061 0 0
BCE INC FOREIGN STOCK 05534B760 229 4,126 SH   DFND 1 4,126 0 0
BCE INC FOREIGN STOCK 05534B760 8 150 SH   OTR 1 150 0 0
BP AMOCO PLC SPONS ADR COMMON STOCK 055622104 214 7,292 SH   DFND 1 7,292 0 0
BANK OF AMERICA COMMON STOCK 060505104 1,237 30,009 SH   DFND 1 30,009 0 0
BIOLIFE SOLUTIONS INC COMMON STOCK 09062W204 1,109 48,775 SH   DFND 1 8,494 0 40,281
BLACKROCK INC COMMON STOCK 09247X101 313 410 SH   DFND 1 410 0 0
BLACKSTONE INC COMMON STOCK 09260D107 211 1,662 SH   DFND 1 1,662 0 0
BLACKSTONE INC COMMON STOCK 09260D107 25 200 SH   OTR 1 200 0 0
BRISTOL MYERS SQUIBB COMMON STOCK 110122108 509 6,974 SH   DFND 1 6,974 0 0
BRISTOL MYERS SQUIBB COMMON STOCK 110122108 49 670 SH   OTR 1 670 0 0
BROADCOM INC COMMON STOCK 11135F101 589 936 SH   DFND 1 936 0 0
CME GROUP INC COMMON STOCK 12572Q105 238 1,000 SH   DFND 1 1,000 0 0
CSX CORP COMMON STOCK 126408103 1,048 27,989 SH   DFND 1 27,989 0 0
CSX CORP COMMON STOCK 126408103 78 2,070 SH   OTR 1 2,070 0 0
CVS HEALTH CORPORATION COMMON STOCK 126650100 629 6,213 SH   DFND 1 6,213 0 0
CHARTER COMMUNICATIONS INC NEW COMMON STOCK 16119P108 450 824 SH   DFND 1 824 0 0
CHEVRON CORPORATION COMMON STOCK 166764100 4,353 26,731 SH   DFND 1 26,731 0 0
CHEVRON CORPORATION COMMON STOCK 166764100 46 285 SH   OTR 1 285 0 0
CISCO SYSTEMS COMMON STOCK 17275R102 415 7,446 SH   DFND 1 7,446 0 0
CISCO SYSTEMS COMMON STOCK 17275R102 25 450 SH   OTR 1 450 0 0
CINTAS CORPORATION COMMON STOCK 172908105 285 670 SH   DFND 1 670 0 0
CITIGROUP INC COMMON STOCK 172967424 249 4,660 SH   DFND 1 4,660 0 0
CITIZENS FINANCIAL GROUP INC COMMON STOCK 174610105 285 6,298 SH   DFND 1 6,298 0 0
CLOROX COMMON STOCK 189054109 195 1,400 SH   DFND 1 1,400 0 0
CLOROX COMMON STOCK 189054109 14 100 SH   OTR 1 100 0 0
COCA COLA COMMON STOCK 191216100 238 3,843 SH   DFND 1 3,843 0 0
COMCAST CORP NEW COMMON STOCK 20030N101 232 4,945 SH   DFND 1 4,945 0 0
COMERICA INC COMMON STOCK 200340107 210 2,324 SH   DFND 1 2,324 0 0
CONOCOPHILLIPS COMMON STOCK 20825C104 313 3,125 SH   DFND 1 3,125 0 0
CONOCOPHILLIPS COMMON STOCK 20825C104 32 320 SH   OTR 1 320 0 0
CORNING INC COMMON STOCK 219350105 254 6,870 SH   DFND 1 6,700 0 170
CORNING INC COMMON STOCK 219350105 11 300 SH   OTR 1 300 0 0
COSTCO WHOLESALE CORP COMMON STOCK 22160K105 923 1,603 SH   DFND 1 1,603 0 0
CUMMINS ENGINE COMMON STOCK 231021106 211 1,028 SH   DFND 1 1,028 0 0
CUMMINS ENGINE COMMON STOCK 231021106 12 60 SH   OTR 1 60 0 0
D R HORTON INC COMMON STOCK 23331A109 333 4,470 SH   DFND 1 4,470 0 0
DANAHER CORP COMMON STOCK 235851102 703 2,397 SH   DFND 1 2,397 0 0
DANAHER CORP COMMON STOCK 235851102 15 51 SH   OTR 1 51 0 0
DEERE & CO COMMON STOCK 244199105 1,554 3,741 SH   DFND 1 3,741 0 0
WALT DISNEY CO COMMON STOCK 254687106 317 2,314 SH   DFND 1 2,314 0 0
DISCOVER FINANCIAL SERVICES COMMON STOCK 254709108 289 2,623 SH   DFND 1 2,623 0 0
DISCOVER FINANCIAL SERVICES COMMON STOCK 254709108 22 200 SH   OTR 1 200 0 0
EOG RESOURCES INC COMMON STOCK 26875P101 209 1,756 SH   DFND 1 1,756 0 0
EDISON INTERNATIONAL COMMON STOCK 281020107 207 2,959 SH   DFND 1 2,959 0 0
ESCALADE INC COMMON STOCK 296056104 1,838 139,208 SH   DFND 1 111,132 28,076 0
EXXON MOBIL CORPORATION COMMON STOCK 30231G102 1,589 19,237 SH   DFND 1 19,237 0 0
EXXON MOBIL CORPORATION COMMON STOCK 30231G102 41 500 SH   OTR 1 500 0 0
META PLATFORMS INC COMMON STOCK 30303M102 1,053 4,736 SH   DFND 1 4,736 0 0
FASTENAL CO COMMON STOCK 311900104 205 3,448 SH   DFND 1 3,448 0 0
FISERV COMMON STOCK 337738108 891 8,790 SH   DFND 1 8,790 0 0
GENERAL DYNAMICS COMMON STOCK 369550108 431 1,786 SH   DFND 1 1,786 0 0
GENERAL MOTORS CO COMMON STOCK 37045V100 310 7,089 SH   DFND 1 7,089 0 0
GENUINE PARTS CO COMMON STOCK 372460105 532 4,220 SH   DFND 1 4,220 0 0
HOME DEPOT INC COMMON STOCK 437076102 1,156 3,861 SH   DFND 1 3,861 0 0
HOME DEPOT INC COMMON STOCK 437076102 37 125 SH   OTR 1 125 0 0
HONEYWELL INTERNATIONAL INC COMMON STOCK 438516106 221 1,134 SH   DFND 1 1,134 0 0
INTEL CORP COMMON STOCK 458140100 316 6,373 SH   DFND 1 6,373 0 0
INTEL CORP COMMON STOCK 458140100 37 750 SH   OTR 1 750 0 0
IBM COMMON STOCK 459200101 354 2,724 SH   DFND 1 2,724 0 0
IBM COMMON STOCK 459200101 39 300 SH   OTR 1 300 0 0
INVESCO MUTUAL FUNDS - 46138G508 1,087 49,951 SH   DFND 1 49,951 0 0
ISHARES MUTUAL FUNDS - 464287101 356 1,705 SH   DFND 1 1,705 0 0
ISHARES MUTUAL FUNDS - 464287168 457 3,570 SH   DFND 1 3,570 0 0
ISHARES MUTUAL FUNDS - 464287168 38 300 SH   OTR 1 300 0 0
ISHARES MUTUAL FUNDS - 464287200 15,455 34,066 SH   DFND 1 34,066 0 0
ISHARES MUTUAL FUNDS - 464287309 8,285 108,472 SH   DFND 1 108,472 0 0
ISHARES MUTUAL FUNDS - 464287408 1,787 11,475 SH   DFND 1 11,475 0 0
ISHARES MUTUAL FUNDS - 464287457 498 5,969 SH   DFND 1 5,969 0 0
ISHARES MUTUAL FUNDS - 464287457 481 5,767 SH   OTR 1 5,767 0 0
ISHARES MUTUAL FUNDS - 464287465 578 7,855 SH   DFND 1 7,855 0 0
ISHARES MUTUAL FUNDS - 464287499 2,487 31,869 SH   DFND 1 31,869 0 0
ISHARES MUTUAL FUNDS - 464287507 10,832 40,368 SH   DFND 1 39,998 0 370
ISHARES MUTUAL FUNDS - 464287549 329 847 SH   DFND 1 847 0 0
ISHARES MUTUAL FUNDS - 464287614 206 741 SH   DFND 1 741 0 0
ISHARES MUTUAL FUNDS - 464287622 4,071 16,280 SH   DFND 1 16,280 0 0
ISHARES MUTUAL FUNDS - 464287655 417 2,030 SH   DFND 1 2,030 0 0
ISHARES MUTUAL FUNDS - 464287689 419 1,597 SH   DFND 1 1,597 0 0
ISHARES MUTUAL FUNDS - 464287721 344 3,343 SH   DFND 1 3,343 0 0
ISHARES MUTUAL FUNDS - 464287804 6,453 59,814 SH   DFND 1 59,114 0 700
ISHARES MUTUAL FUNDS - 464288448 235 7,413 SH   DFND 1 7,413 0 0
ISHARES MUTUAL FUNDS - 464288513 47 568 SH   DFND 1 568 0 0
ISHARES MUTUAL FUNDS - 464288513 517 6,277 SH   OTR 1 6,277 0 0
ISHARES MUTUAL FUNDS - 464288570 706 8,156 SH   DFND 1 6,583 0 1,573
ISHARES MUTUAL FUNDS - 464288588 848 8,327 SH   DFND 1 8,327 0 0
ISHARES MUTUAL FUNDS - 464288638 3,414 62,225 SH   DFND 1 62,225 0 0
ISHARES MUTUAL FUNDS - 464288646 3,569 69,008 SH   DFND 1 69,008 0 0
ISHARES MUTUAL FUNDS - 464288661 5,215 42,729 SH   DFND 1 42,729 0 0
ISHARES MUTUAL FUNDS - 464288802 608 6,283 SH   DFND 1 4,471 0 1,812
ISHARES MUTUAL FUNDS - 46429B747 1,718 16,368 SH   DFND 1 16,368 0 0
ISHARES MUTUAL FUNDS - 46432F842 3,012 43,328 SH   DFND 1 43,328 0 0
ISHARES MUTUAL FUNDS - 46432F859 6,674 137,715 SH   DFND 1 137,715 0 0
ISHARES MUTUAL FUNDS - 46434V407 239 5,446 SH   OTR 1 5,446 0 0
JP MORGAN CHASE & CO COMMON STOCK 46625H100 1,941 14,239 SH   DFND 1 14,050 0 189
JP MORGAN CHASE & CO COMMON STOCK 46625H100 45 330 SH   OTR 1 330 0 0
JOHNSON & JOHNSON COMMON STOCK 478160104 1,421 8,015 SH   DFND 1 8,015 0 0
JOHNSON & JOHNSON COMMON STOCK 478160104 69 390 SH   OTR 1 390 0 0
KROGER CO COMMON STOCK 501044101 480 8,375 SH   DFND 1 8,375 0 0
LENNOX INTL COMMON STOCK 526107107 584 2,264 SH   DFND 1 2,264 0 0
ELI LILLY & CO COMMON STOCK 532457108 761 2,657 SH   DFND 1 2,657 0 0
LOCKHEED MARTIN CORP COMMON STOCK 539830109 203 461 SH   DFND 1 461 0 0
LOWES COMPANY COMMON STOCK 548661107 766 3,790 SH   DFND 1 3,790 0 0
MCDONALDS COMMON STOCK 580135101 745 3,013 SH   DFND 1 3,013 0 0
MCDONALDS COMMON STOCK 580135101 25 100 SH   OTR 1 100 0 0
MERCK & CO COMMON STOCK 58933Y105 774 9,429 SH   DFND 1 9,429 0 0
MERCK & CO COMMON STOCK 58933Y105 71 865 SH   OTR 1 865 0 0
METLIFE COMMON STOCK 59156R108 239 3,404 SH   DFND 1 3,404 0 0
METLIFE COMMON STOCK 59156R108 11 150 SH   OTR 1 150 0 0
MICROSOFT CORP COMMON STOCK 594918104 2,984 9,680 SH   DFND 1 9,680 0 0
MICROSOFT CORP COMMON STOCK 594918104 84 273 SH   OTR 1 273 0 0
MORGAN STANLEY COMMON STOCK 617446448 437 5,000 SH   DFND 1 5,000 0 0
NEXTERA ENERGY INC COMMON STOCK 65339F101 558 6,588 SH   DFND 1 6,588 0 0
NIKE CLASS B COMMON STOCK 654106103 245 1,820 SH   DFND 1 1,820 0 0
NVIDIA CORPORATION COMMON STOCK 67066G104 772 2,830 SH   DFND 1 2,830 0 0
NVIDIA CORPORATION COMMON STOCK 67066G104 23 84 SH   OTR 1 84 0 0
OGE ENERGY COMMON STOCK 670837103 213 5,228 SH   DFND 1 5,228 0 0
OCCIDENTAL PETROLEUM CORP COMMON STOCK 674599105 254 4,475 SH   DFND 1 4,475 0 0
ONEOK INC COMMON STOCK 682680103 274 3,881 SH   DFND 1 3,881 0 0
ORACLE CORPORATION COMMON STOCK 68389X105 870 10,517 SH   DFND 1 10,517 0 0
PNC FINANCIAL SERVICES GROUP INC COMMON STOCK 693475105 242 1,314 SH   DFND 1 1,314 0 0
PPG INDUSTRIES INC COMMON STOCK 693506107 317 2,415 SH   DFND 1 2,415 0 0
PAYCHEX INC COMMON STOCK 704326107 283 2,073 SH   DFND 1 2,073 0 0
PAYPAL COMMON STOCK 70450Y103 205 1,769 SH   DFND 1 1,769 0 0
PAYPAL COMMON STOCK 70450Y103 6 53 SH   OTR 1 53 0 0
PEOPLES BANCORP INC COMMON STOCK 709789101 25,305 808,222 SH   DFND 1 17,240 790,982 0
PEOPLES BANCORP INC COMMON STOCK 709789101 364 11,617 SH   OTR 1 0 11,617 0
PEPSICO INC COMMON STOCK 713448108 900 5,375 SH   DFND 1 5,375 0 0
PEPSICO INC COMMON STOCK 713448108 66 392 SH   OTR 1 392 0 0
PFIZER INC COMMON STOCK 717081103 358 6,923 SH   DFND 1 6,923 0 0
PFIZER INC COMMON STOCK 717081103 72 1,400 SH   OTR 1 1,400 0 0
PHILIP MORRIS INTERNATIONAL INC COMMON STOCK 718172109 197 2,098 SH   DFND 1 2,098 0 0
PHILIP MORRIS INTERNATIONAL INC COMMON STOCK 718172109 19 200 SH   OTR 1 200 0 0
PHILLIPS 66 COMMON STOCK 718546104 680 7,870 SH   DFND 1 7,870 0 0
PHILLIPS 66 COMMON STOCK 718546104 14 160 SH   OTR 1 160 0 0
PIONEER NATURAL RESOURCES COMMON STOCK 723787107 247 986 SH   DFND 1 986 0 0
PROCTER & GAMBLE CO COMMON STOCK 742718109 3,173 20,765 SH   DFND 1 20,765 0 0
PROCTER & GAMBLE CO COMMON STOCK 742718109 149 974 SH   OTR 1 974 0 0
PRUDENTIAL FINANCIAL INC COMMON STOCK 744320102 431 3,646 SH   DFND 1 3,646 0 0
PRUDENTIAL FINANCIAL INC COMMON STOCK 744320102 12 100 SH   OTR 1 100 0 0
QUALCOMM INC COMMON STOCK 747525103 215 1,410 SH   DFND 1 1,410 0 0
RAYTHEON TECHNOLOGIES CORP COMMON STOCK 75513E101 267 2,690 SH   DFND 1 2,690 0 0
RAYTHEON TECHNOLOGIES CORP COMMON STOCK 75513E101 10 100 SH   OTR 1 100 0 0
RIO TINTO PLC ADR COMMON STOCK 767204100 228 2,831 SH   DFND 1 2,831 0 0
S & P GLOBAL INC COMMON STOCK 78409V104 269 655 SH   DFND 1 655 0 0
SPDR MUTUAL FUNDS - 78462F103 6,955 15,399 SH   DFND 1 15,399 0 0
SPDR MUTUAL FUNDS - 78464A474 844 28,008 SH   DFND 1 28,008 0 0
SPDR MUTUAL FUNDS - 78464A763 971 7,577 SH   DFND 1 7,577 0 0
SPDR MUTUAL FUNDS - 78468R408 496 18,896 SH   DFND 1 18,896 0 0
SPDR MUTUAL FUNDS - 78468R408 241 9,194 SH   OTR 1 9,194 0 0
SCHWAB MUTUAL FUNDS - 808524797 5,856 74,230 SH   DFND 1 74,230 0 0
SHERWIN WILLIAMS CO. COMMON COMMON STOCK 824348106 378 1,514 SH   DFND 1 1,514 0 0
STRYKER COMMON STOCK 863667101 399 1,492 SH   DFND 1 1,492 0 0
TEXAS INSTRUMENTS INC COMMON STOCK 882508104 357 1,945 SH   DFND 1 1,945 0 0
TEXAS INSTRUMENTS INC COMMON STOCK 882508104 23 125 SH   OTR 1 125 0 0
TRUIST FINANCIAL CORPORATION COMMON STOCK 89832Q109 514 9,071 SH   DFND 1 9,071 0 0
TRUIST FINANCIAL CORPORATION COMMON STOCK 89832Q109 11 194 SH   OTR 1 194 0 0
TWITTER INC COMMON STOCK 90184L102 242 6,265 SH   DFND 1 6,265 0 0
TYSON FOODS COMMON STOCK 902494103 253 2,823 SH   DFND 1 2,823 0 0
UNION PACIFIC CORP COMMON STOCK 907818108 1,020 3,733 SH   DFND 1 3,733 0 0
UNION PACIFIC CORP COMMON STOCK 907818108 27 100 SH   OTR 1 100 0 0
UNITED BANKSHARES INC COMMON STOCK 909907107 293 8,410 SH   DFND 1 8,410 0 0
UNITEDHEALTH GROUP INC COMMON STOCK 91324P102 702 1,376 SH   DFND 1 1,376 0 0
UNITEDHEALTH GROUP INC COMMON STOCK 91324P102 25 50 SH   OTR 1 50 0 0
VANGUARD MUTUAL FUNDS - 921943858 1,553 32,331 SH   DFND 1 32,331 0 0
VANGUARD MUTUAL FUNDS - 922020805 3,247 63,410 SH   DFND 1 63,410 0 0
VANGUARD MUTUAL FUNDS - 922042858 877 19,013 SH   DFND 1 19,013 0 0
VANGUARD MUTUAL FUNDS - 92204A702 468 1,124 SH   DFND 1 1,124 0 0
VANGUARD MUTUAL FUNDS - 922908769 282 1,240 SH   DFND 1 1,240 0 0
VERIZON COMMUNICATIONS COMMON STOCK 92343V104 476 9,335 SH   DFND 1 9,335 0 0
VERIZON COMMUNICATIONS COMMON STOCK 92343V104 39 765 SH   OTR 1 765 0 0
VISA INC COMMON STOCK 92826C839 329 1,485 SH   DFND 1 1,485 0 0
VISA INC COMMON STOCK 92826C839 12 52 SH   OTR 1 52 0 0
ACCENTURE PLC COMMON STOCK G1151C101 398 1,179 SH   DFND 1 1,179 0 0
LINDE PLC FOREIGN STOCK G5494J103 540 1,690 SH   DFND 1 1,690 0 0
MEDTRONIC PLC COMMON STOCK G5960L103 425 3,831 SH   DFND 1 3,831 0 0