<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4/A</documentType>

    <periodOfReport>2025-05-16</periodOfReport>

    <dateOfOriginalSubmission>2025-05-22</dateOfOriginalSubmission>

    <issuer>
        <issuerCik>0000933972</issuerCik>
        <issuerName>374Water Inc.</issuerName>
        <issuerTradingSymbol>SCWO</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002000149</rptOwnerCik>
            <rptOwnerName>Meyers Brad Ian</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O 374 WATER INC.</rptOwnerStreet1>
            <rptOwnerStreet2>100 SOUTHCENTER COURT, SUITE 200</rptOwnerStreet2>
            <rptOwnerCity>MORRISVILLE</rptOwnerCity>
            <rptOwnerState>NC</rptOwnerState>
            <rptOwnerZipCode>27560</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>true</isOfficer>
            <officerTitle>Chief Operating Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock (restricted stock units)</value>
            </securityTitle>
            <transactionDate>
                <value>2025-05-16</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>115500</value>
                    <footnoteId id="F1"/>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>115500</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeTransaction>
            <securityTitle>
                <value>Restricted Stock Units</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0</value>
            </conversionOrExercisePrice>
            <transactionDate>
                <value>2025-05-16</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>115500</value>
                    <footnoteId id="F1"/>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <exerciseDate>
                <footnoteId id="F3"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F3"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock, $0.0001 par value</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>115500</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>115500</value>
                    <footnoteId id="F2"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeTransaction>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The Restricted Stock Units (the &quot;RSU&quot;) reported herein were granted to the Reporting Person May 16, 2024 (the &quot;Effective Date&quot;) pursuant to his Employment Agreement (the &quot;Employment Agreement&quot;). The RSU grants consisted of an RSU for 115,500 shares that vest over time (the &quot;Time RSU&quot;) and an RSU for 115,500 shares that vest on the achievement of certain milestones (the &quot;Performance RSU&quot;). 28,875 of the shares subject to the Time RSU vested on May 16, 2025, and the remaining 86,625 shares vest in equal increments on the last day of every month thereafter over the following 36 months, subject to the Reporting Person's continued employment with the Issuer on each vesting date. As of September 2, 2025, 36,093 shares subject to the Time RSU were vested, and 79,407 shares are unvested.</footnote>
        <footnote id="F2">On May 22, 2024, the Reporting Person filed a Form 4 that reported both the Time RSU and the Performance RSU in Table II. This amendment serves to remove the Time RSU from Table II and report it in Table I.</footnote>
        <footnote id="F3">Under the Employment Agreement, the Reporting Person was granted the Performance RSU, that vest pursuant to certain milestones set forth by the Issuer.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Brad Ian Meyers</signatureName>
        <signatureDate>2025-09-02</signatureDate>
    </ownerSignature>
</ownershipDocument>
