<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3/A</documentType>

    <periodOfReport>2020-12-01</periodOfReport>

    <dateOfOriginalSubmission>2020-12-03</dateOfOriginalSubmission>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0000093314</issuerCik>
        <issuerName>VOLITIONRX LTD</issuerName>
        <issuerTradingSymbol>VNRX</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001834339</rptOwnerCik>
            <rptOwnerName>Butera Salvatore Thomas</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>13215 BEE CAVE PARKWAY</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 125, GALLERIA OAKS B</rptOwnerStreet2>
            <rptOwnerCity>AUSTIN</rptOwnerCity>
            <rptOwnerState>TX</rptOwnerState>
            <rptOwnerZipCode>78738</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>CEO-Volition Veterinary</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>7000</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F1"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The reporting person inadvertently omitted an additional 2,000 shares held jointly with his spouse in his original Form 3 filing, and such shares were also inadvertently omitted from the Form 4 filed by the reporting person on December 3, 2020. The amount reflected herein includes the corrected aggregate holdings of shares held by the reporting person jointly with his spouse.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Salvatore Thomas Butera</signatureName>
        <signatureDate>2021-05-04</signatureDate>
    </ownerSignature>
</ownershipDocument>
