SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                   FORM 10-K/A
                Annual Report Pursuant to Section 13 or 15(d) of
                       the Securities Exchange Act of 1934

                   For the fiscal year ended December 31, 2000

                           Commission File No. 0-24946


                           KNIGHT TRANSPORTATION, INC.
             (Exact Name of Registrant as Specified in Its Charter)


                 Arizona                                         86-0649974
    (State or Other Jurisdiction of                           (I.R.S. Employer
     Incorporation or Organization)                          Identification No.)


5601 West Buckeye Road, Phoenix, Arizona                           85043
(Address of Principal Executive Offices)                         (Zip Code)


                                 (602) 269-2000
              (Registrant's Telephone Number, Including Area Code)

        Securities Registered Pursuant to Section 12(b) of the Act: None
           Securities Registered Pursuant to Section 12(g) of the Act:


     TITLE OF EACH CLASS                    NAME OF EXCHANGE ON WHICH REGISTERED
     -------------------                    ------------------------------------
Common Stock, $0.01 par value                           NASDAQ-NMS

                                    AMENDMENT

The Annual  Report  filed on Form 10-K  pursuant  to Section 13 or 15 (d) of the
Securities  Exchange  Act of 1934 for the fiscal  year ended  December  31, 2000
filed by Knight Transportation,  Inc. on March 21, 2001 (the "Annual Report") is
amended as follows to correct an erroneous  date shown on the cover page to Form
10-K:

1.   Paragraph  number 3 on the front cover page of the Annual Report is deleted
     in its entirety and replaced with the following, for the purpose of showing
     the correct date:

The  aggregate  market  value of  voting  stock  held by  non-affiliates  of the
registrant  as of March 20,  2001,  was  $179,452,361.25  (based upon $21.25 per
share  being the closing  sale price on that date as  reported  by the  National
Association of Securities  Dealers Automated  Quotation  System-National  Market
System  ("NASDAQ-NMS")).  In making this  calculation,  the issuer has  assumed,
without admitting for any purpose,  that all executive officers and directors of
the company, and no other persons, are affiliates.

2.   Paragraph  number 4 on the front cover page of the Annual Report is deleted
     in its entirety and replaced with the following, for the purpose of showing
     the correct date:

The number of shares  outstanding of the  registrant's  common stock as of March
20, 2001 was approximately 15,384,760.

     Pursuant  to the  requirements  of  Section  13 or 15(d) of the  Securities
Exchange Act of 1934, Knight Transportation, Inc. has duly caused this Amendment
No. 1 to Form  10-K  report  to be  signed  on its  behalf  by the  undersigned,
thereunto duly authorized.


                                   KNIGHT TRANSPORTATION, INC.



Date: March 21, 2001               By: /s/ Kevin P. Knight
                                      ------------------------------------------
                                       Kevin P. Knight, Chief Executive Officer



                                   By: /s/ Timothy M. Kohl
                                      ------------------------------------------
                                       Timothy M. Kohl, Chief Financial Officer,
                                       Secretary