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<!--Created: Thu Mar 14 13:47:59 UTC 2024-->
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      <registrantFullName>Western Southern Life Assurance Co Separate Account 1</registrantFullName>
      <investmentCompFileNo>811-08420</investmentCompFileNo>
      <registrantCik>0000920548</registrantCik>
      <registrantLei>549300IR8CXHCX3PLU96</registrantLei>
      <registrantstreet1>400 Broadway Street, MS 24</registrantstreet1>
      <registrantcity>Cincinnati</registrantcity>
      <registrantzipCode>45202</registrantzipCode>
      <registrantstate>US-OH</registrantstate>
      <registrantcountry>US</registrantcountry>
      <registrantphoneNumber>513 629 1800</registrantphoneNumber>
      <websites>
        <website webpage="https://www.westernsouthern.com"/>
      </websites>
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          <officeName>Jason Nickles</officeName>
          <officeAddress1>400 Broadway Street, MS 24</officeAddress1>
          <officeCity>Cincinnati</officeCity>
          <officeStateCountry officeState="US-OH" officeCountry="US"/>
          <officeRecordsZipCode>45202</officeRecordsZipCode>
          <officePhone>513 629 7418</officePhone>
          <booksRecordsDesc>Charter, By-Laws, Board Minutes., Trial Balance(TB), TB Detail.</booksRecordsDesc>
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      <isRegistrantLastFiling>N</isRegistrantLastFiling>
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          <ccoStreet1>400 Broadway</ccoStreet1>
          <ccoCity>Cincinnati</ccoCity>
          <ccoStateCountry ccoState="US-OH" ccoCountry="US"/>
          <ccoZipCode>45202</ccoZipCode>
          <ccoPhone>XXXXXX</ccoPhone>
          <isCcoChangedSinceLastFiling>N</isCcoChangedSinceLastFiling>
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      <isRegistrantSubmittedMatter>N</isRegistrantSubmittedMatter>
      <isPreviousLegalProceeding>N</isPreviousLegalProceeding>
      <isPreviousProceedingTerminated>N</isPreviousProceedingTerminated>
      <isFinancialSupportDuringPeriod>N</isFinancialSupportDuringPeriod>
      <isExemptionFromAct>N</isExemptionFromAct>
      <principalUnderwriters>
        <principalUnderwriter>
          <principalUnderwriterName>Touchstone Securities, Inc.</principalUnderwriterName>
          <principalUnderwriterFileNumber>8-14052</principalUnderwriterFileNumber>
          <principalUnderwriterCrdNumber>000001526</principalUnderwriterCrdNumber>
          <principalUnderwriterLei>N/A</principalUnderwriterLei>
          <principalUnderWriterStateCountry principalUnderWriterState="US-NE" principalUnderWriterCountry="US"/>
          <isPrincipalUnderwriterAffiliatedWithRegistrant>Y</isPrincipalUnderwriterAffiliatedWithRegistrant>
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      </principalUnderwriters>
      <isUnderwriterHiredOrTerminated>N</isUnderwriterHiredOrTerminated>
      <publicAccountants>
        <publicAccountant>
          <publicAccountantName>ERNST &amp; YOUNG</publicAccountantName>
          <pcaobNumber>(US) 42</pcaobNumber>
          <publicAccountantLei>N/A</publicAccountantLei>
          <publicAccountantStateCountry publicAccountantState="US-DE" publicAccountantCountry="US"/>
        </publicAccountant>
      </publicAccountants>
      <isPublicAccountantChanged>N</isPublicAccountantChanged>
      <isOpinionOffered>N</isOpinionOffered>
      <isMaterialChange>N</isMaterialChange>
      <isAccountingPrincipleChange>N</isAccountingPrincipleChange>
    </registrantInfo>
    <unitInvestmentTrust>
      <depositors>
        <depositor>
          <depositorName>Western Southern Life Assurance Co</depositorName>
          <depositorCrdNo>N/A</depositorCrdNo>
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          <depositorStateCountry depositorState="US-OH" depositorCountry="US"/>
          <depositorUltimateParentname>WESTERN &amp; SOUTHERN FINANCIAL GROUP, INC.</depositorUltimateParentname>
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      <isUitAdminHiredTerminated>N</isUitAdminHiredTerminated>
      <registrantSeparateInsuranceAccount isRegistrantSeparateInsuranceAccount="Y" separateAccountSeriesId="S000000873"/>
      <numOfContracts>408</numOfContracts>
      <contractSecurities>
        <contractSecurity separateAccountSecurityName="Touchstone Gold" separateAccountContractId="C000002490" separateAccountTotalAsset="15922955.03" numContractsSold="0" grossPremiumReceived="129980.99" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="1692062.26" contractValueRedeemedSection1035="302368.38" numContractsAffectedRedeemed="5"/>
        <contractSecurity separateAccountSecurityName="Touchstone Select" separateAccountContractId="C000002491" separateAccountTotalAsset="856080.90" numContractsSold="0" grossPremiumReceived="1200.00" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="52350.11" contractValueRedeemedSection1035="0.00" numContractsAffectedRedeemed="0"/>
        <contractSecurity separateAccountSecurityName="Touchstone Advisor" separateAccountContractId="C000109528" separateAccountTotalAsset="639845.11" numContractsSold="0" grossPremiumReceived="0.00" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="6491.61" contractValueRedeemedSection1035="0.00" numContractsAffectedRedeemed="0"/>
      </contractSecurities>
      <isRule6C7Reliance>N</isRule6C7Reliance>
      <isRule11A2Reliance>N</isRule11A2Reliance>
      <isRule12D1Dash4Reliance>N</isRule12D1Dash4Reliance>
      <isRule12D1GReliance>N</isRule12D1GReliance>
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      <isLegalProceedings>false</isLegalProceedings>
      <isProvisionFinancialSupport>false</isProvisionFinancialSupport>
      <isIPAReportInternalControl>false</isIPAReportInternalControl>
      <isInfoRequiredEO>false</isInfoRequiredEO>
      <isOtherInfoRequired>false</isOtherInfoRequired>
    </attachmentsTab>
    <signature registrantSignedName="Western Southern Life Assurance Co Separate Account 1" signedDate="2024-03-14" signature="Wade M Fugate" title="Vice President and Controller"/>
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