Exhibit 16.1

March 3, 2021

Securities and Exchange Commission

100 F Street, N.E.

Washington, DC 20549

Commissioners:

We have read the statements made by Laboratory Corporation of America Holdings (copy attached), which we understand will be filed with the Securities and Exchange Commission, pursuant to Item 4.01 of Form 8-K/A of Laboratory Corporation of America Holdingsdated November 2, 2020.We agree with the statements concerning our Firm contained therein.

Very truly yours,

/s/ PricewaterhouseCoopers LLP

Raleigh, North Carolina

Attachment