Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in this Amendment No. 1 to Registration Statement on Form S-3 of QCR Holdings, Inc. and subsidiaries of our reports dated March 12, 2015, relating to our audits of the consolidated financial statements and internal control over financial reporting which appears in the Annual Report on Form 10-K of QCR Holdings, Inc. and subsidiaries for the year ended December 31, 2014.

We also consent to the reference to our Firm under the caption “Experts” in the Prospectus, which is part of this Amendment No. 1 to Registration Statement.

/s/ McGladrey LLP

Davenport, Iowa

September 24, 2015