Exhibit 1(u)

TWENTIETH AMENDMENT DATED APRIL 28, 2017

TO JANUS ASPEN SERIES’

AMENDED AND RESTATED TRUST INSTRUMENT

DATED MARCH 18, 2003

AND AMENDED DECEMBER 29, 2005

Pursuant to authority granted by the Trustees, Schedule A of the Amended and Restated Trust Instrument, as amended, is further amended effective May 1, 2017, to reflect the name change of Janus Portfolio to Research Portfolio.

SCHEDULE A

Series of the Trust	Available Classes
Balanced Portfolio	Institutional Shares
	Service Shares
Enterprise Portfolio	Institutional Shares
	Service Shares
Flexible Bond Portfolio	Institutional Shares
	Service Shares
Forty Portfolio	Institutional Shares
	Service Shares
Global Technology Portfolio	Institutional Shares
	Service Shares
Global Research Portfolio	Institutional Shares
	Service Shares
Overseas Portfolio	Institutional Shares
	Service Shares
Research Portfolio	Institutional Shares
	Service Shares
Janus Aspen INTECH U.S. Low Volatility Portfolio	Service Shares
Janus Aspen Perkins Mid Cap Value Portfolio	Institutional Shares
	Service Shares
Global Allocation Portfolio - Moderate	Institutional Shares
	Service Shares
Global Unconstrained Bond Portfolio	Institutional Shares Service Shares

Global Bond Portfolio	Institutional Shares
	Service Shares
[SEAL]