<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0000906185</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


      <seriesClassInfo>
        <seriesId>S000010406</seriesId>
        <classId>C000028737</classId>
        <classId>C000028736</classId>
      </seriesClassInfo>


    </filerInfo>
  </headerData>
  <formData>
    <genInfo>
      <regName>JANUS ASPEN SERIES</regName>
      <regFileNumber>811-07736</regFileNumber>
      <regCik>0000906185</regCik>
      <regLei>5C1H794C69TI10A0XC04</regLei>
      <regStreet1>151 DETROIT STREET</regStreet1>
      <regCity>Denver</regCity>
      <regStateConditional regCountry="US" regState="US-CO"/>
      <regZipOrPostalCode>80206</regZipOrPostalCode>
      <regPhone>303-333-3863</regPhone>
      <seriesName>Janus Henderson Forty Portfolio</seriesName>
      <seriesId>S000010406</seriesId>
      <seriesLei>RQXX4TO67B0GELHZYY98</seriesLei>
      <repPdEnd>2026-12-31</repPdEnd>
      <repPdDate>2026-03-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>850050490.079999900000</totAssets>
      <totLiabs>531244.980000000000</totLiabs>
      <netAssets>849519245.100000000000</netAssets>
      <assetsAttrMiscSec>0.000000000000</assetsAttrMiscSec>
      <assetsInvested>0.000000000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.000000000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.000000000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.000000000000</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0.000000000000</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0.000000000000</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0.000000000000</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0.000000000000</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0.000000000000</amtPayAftOneYrOther>
      <delayDeliv>0.000000000000</delayDeliv>
      <standByCommit>0.000000000000</standByCommit>
      <liquidPref>0.000000000000</liquidPref>
      <cshNotRptdInCorD>0.000000000000</cshNotRptdInCorD>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000028736" rtn1="-2.78" rtn2="-5.22" rtn3="-4.81"/>
          <monthlyTotReturn classId="C000028737" rtn1="-2.76" rtn2="-5.17" rtn3="-4.80"/>
        </monthlyTotReturns>
        <othMon1 netRealizedGain="-581764" netUnrealizedAppr="-25744331"/>
        <othMon2 netRealizedGain="19650187" netUnrealizedAppr="-68553608"/>
        <othMon3 netRealizedGain="6495704" netUnrealizedAppr="-49364130"/>
      </returnInfo>
      <mon1Flow redemption="18210492.78" reinvestment="0.00" sales="6901624.90"/>
      <mon2Flow redemption="10446129.70" reinvestment="0.00" sales="7606730.16"/>
      <mon3Flow redemption="7394830.78" reinvestment="0.00" sales="8425427.49"/>



      <varInfo>

        <fundsDesignatedInfo>
          <nameDesignatedIndex>Russell 1000 Growth Total Return Index</nameDesignatedIndex>
          <indexIdentifier>RUSSELL.MC.RLG</indexIdentifier>

        </fundsDesignatedInfo>

      </varInfo>
    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>Oracle Corp.</name>
        <lei>1Z4GXXU7ZHVWFCD8TV52</lei>
        <title>Oracle Corp. COM USD0.01</title>
        <cusip>68389X105</cusip>
        <identifiers>
          <isin value="US68389X1054"/>
          <ticker value="ORCL"/>
          <other otherDesc="SEDOL" value="2661568"/>
        </identifiers>
        <balance>226377</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>33302320.47</valUSD>
        <pctVal>3.9201372614</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Palantir Technologies Inc</name>
        <lei>549300UVN46B3BBDHO85</lei>
        <title>Palantir Technologies Inc CL A</title>
        <cusip>69608A108</cusip>
        <identifiers>
          <isin value="US69608A1088"/>
          <ticker value="PLTR"/>
          <other otherDesc="SEDOL" value="BN78DQ4"/>
        </identifiers>
        <balance>69048</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>10100341.44</valUSD>
        <pctVal>1.1889479253</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Shopify Inc</name>
        <lei>549300HGQ43STJLLP808</lei>
        <title>Shopify Inc COM NPV CL A</title>
        <cusip>82509L107</cusip>
        <identifiers>
          <isin value="CA82509L1076"/>
          <ticker value="SHOP"/>
          <other otherDesc="SEDOL" value="BXDZ9Z0"/>
        </identifiers>
        <balance>97801</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>11601154.62</valUSD>
        <pctVal>1.3656141031</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Taiwan Semiconductor Manufacturing Co., Ltd.</name>
        <lei>549300KB6NK5SBD14S87</lei>
        <title>Taiwan Semiconductor Manufacturing Co., Ltd. SPON ADS REP 5 ORD TWD10</title>
        <cusip>874039100</cusip>
        <identifiers>
          <isin value="US8740391003"/>
          <ticker value="TSM"/>
          <other otherDesc="SEDOL" value="2113382"/>
        </identifiers>
        <balance>137000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>46299150</valUSD>
        <pctVal>5.4500413342</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="GDR"/>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Alphabet Inc.</name>
        <lei>5493006MHB84DD0ZWV18</lei>
        <title>Alphabet Inc. CAP USD0.001 CL C</title>
        <cusip>02079K107</cusip>
        <identifiers>
          <isin value="US02079K1079"/>
          <ticker value="GOOG"/>
          <other otherDesc="SEDOL" value="BYY88Y7"/>
        </identifiers>
        <balance>136862</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>39260233.32</valUSD>
        <pctVal>4.6214648516</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Amazon.com, Inc.</name>
        <lei>ZXTILKJKG63JELOEG630</lei>
        <title>Amazon.com, Inc. COM USD0.01</title>
        <cusip>023135106</cusip>
        <identifiers>
          <isin value="US0231351067"/>
          <ticker value="AMZN"/>
          <other otherDesc="SEDOL" value="2000019"/>
        </identifiers>
        <balance>347635</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>72401941.45</valUSD>
        <pctVal>8.5226958503</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Apple Inc.</name>
        <lei>HWUPKR0MPOU8FGXBT394</lei>
        <title>Apple Inc. COM USD0.00001</title>
        <cusip>037833100</cusip>
        <identifiers>
          <isin value="US0378331005"/>
          <ticker value="AAPL"/>
          <other otherDesc="SEDOL" value="2046251"/>
        </identifiers>
        <balance>184252</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>46761315.08</valUSD>
        <pctVal>5.5044444666</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Chipotle Mexican Grill Inc</name>
        <lei>529900REP5VGTPCP1J71</lei>
        <title>Chipotle Mexican Grill Inc COM USD0.01</title>
        <cusip>169656105</cusip>
        <identifiers>
          <isin value="US1696561059"/>
          <ticker value="CMG"/>
          <other otherDesc="SEDOL" value="B0X7DZ3"/>
        </identifiers>
        <balance>236856</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>7581760.56</valUSD>
        <pctVal>0.8924766100</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Applovin Corp.</name>
        <lei>549300LLVXMUAOL3SQ07</lei>
        <title>Applovin Corp. COM CL A</title>
        <cusip>03831W108</cusip>
        <identifiers>
          <isin value="US03831W1080"/>
          <ticker value="APP"/>
          <other otherDesc="SEDOL" value="BMV3LG4"/>
        </identifiers>
        <balance>20042</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>7976716</valUSD>
        <pctVal>0.9389682513</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Argenx SE</name>
        <lei>7245009C5FZE6G9ODQ71</lei>
        <title>Argenx SE SPON ADR EACH REP 1 ORD SHS</title>
        <cusip>04016X101</cusip>
        <identifiers>
          <isin value="US04016X1019"/>
          <ticker value="ARGX"/>
          <other otherDesc="SEDOL" value="BDVLM39"/>
        </identifiers>
        <balance>22062</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>16110775.5</valUSD>
        <pctVal>1.8964579782</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="GDR"/>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Blackstone Inc</name>
        <lei>5299004LW4QWGZUB8Y96</lei>
        <title>Blackstone Inc COM USD0.00001 CLASS A</title>
        <cusip>09260D107</cusip>
        <identifiers>
          <isin value="US09260D1072"/>
          <ticker value="BX"/>
          <other otherDesc="SEDOL" value="BKF2SL7"/>
        </identifiers>
        <balance>76656</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8814673.44</valUSD>
        <pctVal>1.0376072692</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Boeing Co.</name>
        <lei>RVHJWBXLJ1RFUBSY1F30</lei>
        <title>Boeing Co. COM USD5.00</title>
        <cusip>097023105</cusip>
        <identifiers>
          <isin value="US0970231058"/>
          <ticker value="BA"/>
          <other otherDesc="SEDOL" value="2108601"/>
        </identifiers>
        <balance>79687</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>15860103.61</valUSD>
        <pctVal>1.8669504783</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Danaher Corporation</name>
        <lei>S4BKK9OTCEWQ3YHPFM11</lei>
        <title>Danaher Corporation COM USD0.01</title>
        <cusip>235851102</cusip>
        <identifiers>
          <isin value="US2358511028"/>
          <ticker value="DHR"/>
          <other otherDesc="SEDOL" value="2250870"/>
        </identifiers>
        <balance>81186</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>15392865.6</valUSD>
        <pctVal>1.8119501929</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Datadog Inc</name>
        <lei>549300F6JNO0KRPO1K63</lei>
        <title>Datadog Inc COM USD0.00001 CL A</title>
        <cusip>23804L103</cusip>
        <identifiers>
          <isin value="US23804L1035"/>
          <ticker value="DDOG"/>
          <other otherDesc="SEDOL" value="BKT9Y49"/>
        </identifiers>
        <balance>143838</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>16980075.9</valUSD>
        <pctVal>1.9987864899</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Booking Holdings Inc</name>
        <lei>FXM8FAOHMYDIPD38UZ17</lei>
        <title>Booking Holdings Inc COM USD0.008</title>
        <cusip>09857L108</cusip>
        <identifiers>
          <isin value="US09857L1089"/>
          <ticker value="BKNG"/>
          <other otherDesc="SEDOL" value="BDRXDB4"/>
        </identifiers>
        <balance>3307</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>13923528.24</valUSD>
        <pctVal>1.6389891483</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Broadcom Inc</name>
        <lei>549300WV6GIDOZJTV909</lei>
        <title>Broadcom Inc COM USD0.001</title>
        <cusip>11135F101</cusip>
        <identifiers>
          <isin value="US11135F1012"/>
          <ticker value="AVGO"/>
          <other otherDesc="SEDOL" value="BDZ78H9"/>
        </identifiers>
        <balance>153678</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>47564877.78</valUSD>
        <pctVal>5.5990347546</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>DoorDash Inc</name>
        <lei>549300NUQ43FGGSK5051</lei>
        <title>DoorDash Inc CL A</title>
        <cusip>25809K105</cusip>
        <identifiers>
          <isin value="US25809K1051"/>
          <ticker value="DASH"/>
          <other otherDesc="SEDOL" value="BN13P03"/>
        </identifiers>
        <balance>76208</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>11442631.2</valUSD>
        <pctVal>1.3469537348</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>DraftKings Inc.</name>
        <lei>2549006G33IUL6IVOD83</lei>
        <title>DraftKings Inc. COM CL A</title>
        <cusip>26142V105</cusip>
        <identifiers>
          <isin value="US26142V1052"/>
          <ticker value="DKNG"/>
          <other otherDesc="SEDOL" value="BLDDH12"/>
        </identifiers>
        <balance>861639</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>18628635.18</valUSD>
        <pctVal>2.1928443985</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Meta Platforms Inc</name>
        <lei>BQ4BKCS1HXDV9HN80Z93</lei>
        <title>Meta Platforms Inc COM USD0.000006 CL 'A'</title>
        <cusip>30303M102</cusip>
        <identifiers>
          <isin value="US30303M1027"/>
          <ticker value="META"/>
          <other otherDesc="SEDOL" value="B7TL820"/>
        </identifiers>
        <balance>53391</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>30546592.83</valUSD>
        <pctVal>3.5957505385</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>GE Vernova Inc.</name>
        <lei>254900DP080RU6OK2553</lei>
        <title>GE Vernova Inc. COM</title>
        <cusip>36828A101</cusip>
        <identifiers>
          <isin value="US36828A1016"/>
          <ticker value="GEV"/>
          <other otherDesc="SEDOL" value="BP6H4Y1"/>
        </identifiers>
        <balance>21331</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>18619829.9</valUSD>
        <pctVal>2.1918078969</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>UnitedHealth Group Inc.</name>
        <lei>549300GHBMY8T5GXDE41</lei>
        <title>UnitedHealth Group Inc. COM USD0.01</title>
        <cusip>91324P102</cusip>
        <identifiers>
          <isin value="US91324P1021"/>
          <ticker value="UNH"/>
          <other otherDesc="SEDOL" value="2917766"/>
        </identifiers>
        <balance>53016</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>14345599.44</valUSD>
        <pctVal>1.6886726843</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vaxcyte Inc</name>
        <lei>254900M7NMHTGASX8D95</lei>
        <title>Vaxcyte Inc COM</title>
        <cusip>92243G108</cusip>
        <identifiers>
          <isin value="US92243G1085"/>
          <ticker value="PCVX"/>
          <other otherDesc="SEDOL" value="BKPVGH6"/>
        </identifiers>
        <balance>91415</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>5312125.65</valUSD>
        <pctVal>0.6253096302</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Howmet Aerospace Inc</name>
        <lei>549300HO5WFZUT5N2T22</lei>
        <title>Howmet Aerospace Inc COM</title>
        <cusip>443201108</cusip>
        <identifiers>
          <isin value="US4432011082"/>
          <ticker value="HWM"/>
          <other otherDesc="SEDOL" value="BKLJ8V2"/>
        </identifiers>
        <balance>127866</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>29467998.36</valUSD>
        <pctVal>3.4687852606</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Intercontinental Exchange Inc</name>
        <lei>5493000F4ZO33MV32P92</lei>
        <title>Intercontinental Exchange Inc COM USD0.01</title>
        <cusip>45866F104</cusip>
        <identifiers>
          <isin value="US45866F1049"/>
          <ticker value="ICE"/>
          <other otherDesc="SEDOL" value="BFSSDS9"/>
        </identifiers>
        <balance>69186</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>10881574.08</valUSD>
        <pctVal>1.2809096607</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Lam Research Corporation</name>
        <lei>549300I4GMO6D34U1T02</lei>
        <title>Lam Research Corporation COM NEW</title>
        <cusip>512807306</cusip>
        <identifiers>
          <isin value="US5128073062"/>
          <ticker value="LRCX"/>
          <other otherDesc="SEDOL" value="BSML4N7"/>
        </identifiers>
        <balance>37332</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>7976355.12</valUSD>
        <pctVal>0.9389257708</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Eli Lilly &amp; Co.</name>
        <lei>FRDRIPF3EKNDJ2CQJL29</lei>
        <title>Eli Lilly &amp; Co. COM NPV</title>
        <cusip>532457108</cusip>
        <identifiers>
          <isin value="US5324571083"/>
          <ticker value="LLY"/>
          <other otherDesc="SEDOL" value="2516152"/>
        </identifiers>
        <balance>33176</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>30514289.52</valUSD>
        <pctVal>3.5919479984</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Madrigal Pharmaceuticals Inc</name>
        <lei>549300CHIMT2EUSNJB60</lei>
        <title>Madrigal Pharmaceuticals Inc USD0.0001</title>
        <cusip>558868105</cusip>
        <identifiers>
          <isin value="US5588681057"/>
          <ticker value="MDGL"/>
          <other otherDesc="SEDOL" value="BD59BS7"/>
        </identifiers>
        <balance>17328</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>9070688.16</valUSD>
        <pctVal>1.0677436930</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>MasterCard Incorporated</name>
        <lei>AR5L2ODV9HN37376R084</lei>
        <title>MasterCard Incorporated COM USD0.0001 CLASS A</title>
        <cusip>57636Q104</cusip>
        <identifiers>
          <isin value="US57636Q1040"/>
          <ticker value="MA"/>
          <other otherDesc="SEDOL" value="B121557"/>
        </identifiers>
        <balance>37577</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>18775723.82</valUSD>
        <pctVal>2.2101587372</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Medline Inc.</name>
        <lei>984500FAN6J1P8566D43</lei>
        <title>Medline Inc. COM CL A</title>
        <cusip>58507V107</cusip>
        <identifiers>
          <isin value="US58507V1070"/>
          <ticker value="MDLN"/>
          <other otherDesc="SEDOL" value="BNBV4B1"/>
        </identifiers>
        <balance>127095</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>5655727.5</valUSD>
        <pctVal>0.6657562536</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Mercadolibre Inc</name>
        <lei>549300DKPDN9M5S8GB14</lei>
        <title>Mercadolibre Inc COM USD0.001</title>
        <cusip>58733R102</cusip>
        <identifiers>
          <isin value="US58733R1023"/>
          <ticker value="MELI"/>
          <other otherDesc="SEDOL" value="B23X1H3"/>
        </identifiers>
        <balance>13748</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>23770566.96</valUSD>
        <pctVal>2.7981198892</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Microsoft Corp.</name>
        <lei>INR2EJN1ERAN0W5ZP974</lei>
        <title>Microsoft Corp. COM USD0.00000625</title>
        <cusip>594918104</cusip>
        <identifiers>
          <isin value="US5949181045"/>
          <ticker value="MSFT"/>
          <other otherDesc="SEDOL" value="2588173"/>
        </identifiers>
        <balance>155237</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>57464080.29</valUSD>
        <pctVal>6.7643058849</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>NVIDIA Corp.</name>
        <lei>549300S4KLFTLO7GSQ80</lei>
        <title>NVIDIA Corp. COM USD0.001</title>
        <cusip>67066G104</cusip>
        <identifiers>
          <isin value="US67066G1040"/>
          <ticker value="NVDA"/>
          <other otherDesc="SEDOL" value="2379504"/>
        </identifiers>
        <balance>620733</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>108255835.2</valUSD>
        <pctVal>12.7431880825</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="895"/>
        </identifiers>
        <balance>8487817.4561</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8487817.46</valUSD>
        <pctVal>0.9991318630</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Eaton Corporation PLC</name>
        <lei>549300VDIGTMXUNT7H71</lei>
        <title>Eaton Corporation PLC COM USD0.01</title>
        <cusip>G29183103</cusip>
        <identifiers>
          <isin value="IE00B8KQN827"/>
          <ticker value="ETN"/>
          <other otherDesc="SEDOL" value="B8KQN82"/>
        </identifiers>
        <balance>85881</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>30717057.27</valUSD>
        <pctVal>3.6158165277</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-05-15</ncom:dateSigned>
      <ncom:nameOfApplicant>JANUS ASPEN SERIES</ncom:nameOfApplicant>
      <ncom:signature>JESPER NERGAARD</ncom:signature>
      <ncom:signerName>JESPER NERGAARD</ncom:signerName>
      <ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
