<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2022-03-22</periodOfReport>

    <issuer>
        <issuerCik>0000880242</issuerCik>
        <issuerName>BIOLARGO, INC.</issuerName>
        <issuerTradingSymbol>BLGO</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001112799</rptOwnerCik>
            <rptOwnerName>DARGAN CHARLES K</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>18851 NE 29TH AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 700</rptOwnerStreet2>
            <rptOwnerCity>AVENTURA</rptOwnerCity>
            <rptOwnerState>FL</rptOwnerState>
            <rptOwnerZipCode>3318</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>1</isOfficer>
            <officerTitle>Chief Financial Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <derivativeTable>
        <derivativeTransaction>
            <securityTitle>
                <value>Option to purchase common stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.24</value>
            </conversionOrExercisePrice>
            <transactionDate>
                <value>2022-03-22</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>300000</value>
                </transactionShares>
                <transactionPricePerShare>
                    <footnoteId id="F2"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <exerciseDate>
                <value>2022-03-22</value>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2032-03-22</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>300000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>3959000</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeTransaction>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The Option is exercisable according to its vesting schedule: (i) 25,000 shares vested as of March 22, 2022, and then (ii) in equal monthly installments of 25,000 shares beginning March 31, 2022, so long as the Engagement Extension Agreement dated March 20, 2022, between Reporting Owner and Issuer, remains in force and effect.</footnote>
        <footnote id="F2">This Option was issued to the Reporting Person as compensation to serve as Issuer's Chief Financial Officer from February 1, 2022, through January 31, 2023.</footnote>
    </footnotes>

    <remarks>This transaction was disclosed by the Issuer on Form 8-K filed with the SEC on March 24, 2022.</remarks>

    <ownerSignature>
        <signatureName>/s/ John R. Browning, Attorney-in-fact</signatureName>
        <signatureDate>2022-03-28</signatureDate>
    </ownerSignature>
</ownershipDocument>
