Exhibit 21.1

LIST OF SUBSIDIARIES OF LANDSTAR SYSTEM, INC.

(as of December 30, 2023)

Name	Jurisdiction of Incorporation		% of Voting Securities Owned
Subsidiary of Landstar System, Inc.
Landstar System Holdings, Inc.		Delaware		100
Subsidiaries of Landstar System Holdings, Inc.
Landstar Inway, Inc.		Delaware		100
Landstar Global Logistics, Inc.		Delaware		100
Landstar Ligon, Inc.		Delaware		100
Landstar Ranger, Inc.		Delaware		100
Risk Management Claim Services, Inc.		Delaware		100
Landstar Transportation Logistics, Inc.		Delaware		100
Also d/b/a Landstar Carrier Services, Inc.
Landstar Contractor Financing, Inc.		Delaware		100
Signature Insurance Company		Cayman Islands, BWI		100
Landstar Canada Holdings, Inc.		Delaware		100
Landstar MH I LLC		Delaware		100
Landstar Blue LLC		Delaware		100
Landstar Investment Holdco, LLC		Delaware		100
Subsidiary of Landstar Canada Holdings, Inc.
Landstar Canada, Inc.		Ontario, Canada		100
Also d/b/a Enterprise Landstar Canada in Quebec
Subsidiary of Landstar Global Logistics, Inc.
Landstar Express America, Inc.		Delaware		100
Subsidiary of Landstar Ranger, Inc.
Landstar Gemini, Inc.		Delaware		100
Also d/b/a Landstar Less Than Truck Load
Also d/b/a Landstar LTL
Subsidiary of Landstar MH I LLC
Landstar MH II LLC		Delaware		100
Landstar Holdings, S. de R.L.C.V.		Mexico		0.1
Subsidiary of Landstar MH II LLC
Landstar Holdings, S. de R.L.C.V.		Mexico		99.9
Subsidiary of Landstar Holdings, S. de R.L.C.V.
Landstar Metro, S.A.P.I. de C.V.		Mexico		100