<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2017-08-01</periodOfReport>

    <noSecuritiesOwned>1</noSecuritiesOwned>

    <issuer>
        <issuerCik>0000827809</issuerCik>
        <issuerName>NOVELION THERAPEUTICS INC.</issuerName>
        <issuerTradingSymbol>NVLN</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001669816</rptOwnerCik>
            <rptOwnerName>DiPaolo Mark</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NOVELION THERAPEUTICS INC.</rptOwnerStreet1>
            <rptOwnerStreet2>887 GREAT NORTHERN WAY, SUITE 250</rptOwnerStreet2>
            <rptOwnerCity>VANCOUVER</rptOwnerCity>
            <rptOwnerState>A1</rptOwnerState>
            <rptOwnerZipCode>V5T 4T5</rptOwnerZipCode>
            <rptOwnerStateDescription>BRITISH COLUMBIA, CANADA</rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <remarks>See Exhibit 24.1 - Power of Attorney.</remarks>

    <ownerSignature>
        <signatureName>/s/ Jennifer Fitzpatrick, attorney-in-fact</signatureName>
        <signatureDate>2017-08-02</signatureDate>
    </ownerSignature>
</ownershipDocument>
