<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2024-08-12</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0000803578</issuerCik>
        <issuerName>FIREFLY NEUROSCIENCE, INC.</issuerName>
        <issuerTradingSymbol>AIFF</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002061392</rptOwnerCik>
            <rptOwnerName>Issachar Gil</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O FIREFLY NEUROSCIENCE, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>1100 MILITARY ROAD</rptOwnerStreet2>
            <rptOwnerCity>KENMORE</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>14217</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>1</isOfficer>
            <officerTitle>Chief Technology Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>33480</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
                <footnoteId id="F1"/>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>721.1500</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2018-08-13</value>
            </exerciseDate>
            <expirationDate>
                <value>2023-08-13</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>13</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
                <footnoteId id="F2"/>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>72.1200</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2020-10-14</value>
            </exerciseDate>
            <expirationDate>
                <value>2025-10-14</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>222</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
                <footnoteId id="F3"/>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>72.1200</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2021-04-01</value>
            </exerciseDate>
            <expirationDate>
                <value>2026-04-01</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>77</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
                <footnoteId id="F4"/>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>72.1200</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2021-06-21</value>
            </exerciseDate>
            <expirationDate>
                <value>2026-06-21</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>1308</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
                <footnoteId id="F5"/>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>28.8200</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2022-11-17</value>
            </exerciseDate>
            <expirationDate>
                <value>2027-11-17</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>2920</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Employee Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>5.1800</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F6"/>
            </exerciseDate>
            <expirationDate>
                <value>2028-07-08</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>75418</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Gil Issachar was granted an option to purchase 13 shares of common stock (&quot;Option Shares&quot;) at an exercisable price of $721.15 per share on August 13, 2018.</footnote>
        <footnote id="F2">Gil Issachar was granted an option to purchase 222 shares of common stock (&quot;Option Shares&quot;) at an exercisable price of $72.12 per share on October 14, 2020.</footnote>
        <footnote id="F3">Gil Issachar was granted an option to purchase 77 shares of common stock (&quot;Option Shares&quot;) at an exercisable price of $72.12 per share on April 1, 2021.</footnote>
        <footnote id="F4">Gil Issachar was granted an option to purchase 1,308 shares of common stock (&quot;Option Shares&quot;) at an exercisable price of $72.12 per share on June 21, 2021.</footnote>
        <footnote id="F5">Gil Issachar was granted an option to purchase 2,920 shares of common stock (&quot;Option Shares&quot;) at an exercisable price of $28.82 per share on November 17, 2022.</footnote>
        <footnote id="F6">Gil Issachar was granted an option to purchase 75,418 shares of common stock (&quot;Option Shares&quot;) subject a vesting schedule, in which the 75,418 Option Shares shall be vested in equal installment at the end of each calendar month for 36 months began on the date of grant on July 8, 2023.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Gil Issachar</signatureName>
        <signatureDate>2025-03-19</signatureDate>
    </ownerSignature>
</ownershipDocument>
