U.S. SECURITIES AND EXCHANGE COMMISSION
WASHINGTON, D.C. 20549

FORM 12b-25

SEC File Number 000-51808

CUSIP Number 381312 10 7

NOTICE OF LATE FILING

(Check One):

[  ] Form 10-K  [ ] Form 11-K  [ ] Form 20-F  [ x  ] Form 10-Q  [ ] Form N-SAR

For Period Ended:  April 30, 2010

If the notification relates to a portion of the filing checked above, identify the Item(s) to which the notification relates:     N/A

Part I--Registrant Information

           Full Name of Registrant:

VITRO DIAGNOSTICS, INC

          Former Name if Applicable:

          Address of Principal Executive Office (Street and Number):

4621 Technology Drive, Golden, CO  80403

Part II--Rules 12b-25(b) and (c)

If the subject report could not be filed without unreasonable effort or expense and the registrant seeks relief pursuant to Rule 12(b)-25(b), the following should be completed. (Check box if appropriate)

(a)	[ ]	The reasons described in reasonable detail in Part III of this form could not be eliminated without unreasonable effort or expense;
(b)	[X]	The subject annual report or semi-annual report/portion thereof will be filed on or before the fifteenth calendar day following the prescribed due date; or the subject quarterly report/portion thereof will be filed on or before the fifth calendar day following the prescribed due date; and
(c)	[ ]	The accountant's statement or other exhibit required by Rule 12b-25(c) has been attached if applicable.

Part III--Narrative

State below in reasonable detail the reasons why form 10-K, 11-K, 20-F, 10-Q or N-SAR or portion thereof could not be filed within the prescribed time period.

The Registrant is unable to file its Quarterly Report on Form 10-Q within the prescribed time period because the Company has not completed the preparation of its unaudited financial statements for the fiscal quarter.

Part IV--Other Information

(1)

Name and telephone number of person to contact in regard to this notification

Clifford L. Neuman, P.C.
1507 Pine Street
Boulder, Colorado 80302
(303) 449-2100

(2)

Have all other period reports required under section 13 or 15(d) of the Securities Exchange Act of 1934 or section 30 of the Investment Company Act of 1940 during the preceding 12 months or for such shorter period that the registrant was required to file such report(s) been filed? If the answer is no, identify report(s).

[ X] Yes [ ] No

(3)

Is it anticipated that any significant change in results of operations from the corresponding period for the last fiscal year will be reflected by the earnings statements to be included in the subject report or portion thereof?

[ ] Yes [X] No

If so; attach an explanation of the anticipated change, both narratively and quantitively, and, if appropriate, state the reasons why a reasonable estimate of the results cannot be made.

         VITRO DIAGNOSTICS, INC.       
(Name of Registrant as specified in charter)

has caused this notification to be signed on its behalf by the undersigned thereunto duly authorized.

Date:        June 14, 2010	By__/s/ James R. Musick____________
	James R. Musick, President, Chairman of the Board, Chief Executive Officer, Principal Financial and Accounting Officer and Director