<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
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    <isConfidential>false</isConfidential>
    <filerInfo>

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        <issuerCredentials>
          <cik>0000773674</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


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        <classId>C000016438</classId>
        <classId>C000088038</classId>
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        <classId>C000181796</classId>
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        <classId>C000181797</classId>
      </seriesClassInfo>


    </filerInfo>
  </headerData>
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      <regName>American Century Government Income Trust</regName>
      <regFileNumber>811-04363</regFileNumber>
      <regCik>0000773674</regCik>
      <regLei>5493006ATS1M2OACE377</regLei>
      <regStreet1>4500 Main St</regStreet1>
      <regCity>Kansas City</regCity>
      <regStateConditional regCountry="US" regState="US-MO"/>
      <regZipOrPostalCode>64111</regZipOrPostalCode>
      <regPhone>816-531-5575</regPhone>
      <seriesName>Inflation-Adjusted Bond Fund</seriesName>
      <seriesId>S000005969</seriesId>
      <seriesLei>EHDCH7OW63U5SCJHI161</seriesLei>
      <repPdEnd>2020-03-31</repPdEnd>
      <repPdDate>2019-12-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
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      <totLiabs>23233363.310000000000</totLiabs>
      <netAssets>2530566973.560000000000</netAssets>
      <assetsAttrMiscSec>0.000000000000</assetsAttrMiscSec>
      <assetsInvested>0.000000000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.000000000000</amtPayOneYrBanksBorr>
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      <curMetrics>
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    </fundInfo>
    <invstOrSecs>
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        <name>ABN Amro Mortgage Corp</name>
        <lei>N/A</lei>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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        <lei>N/A</lei>
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        <name>BRE Grand Islander Timeshare Issuer 2017-A LLC</name>
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        <name>LELAND STANFORD JR UNIV</name>
        <lei>JIUV0DC2REIJXQ26CR37</lei>
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        <name>COMM 2014-CCRE15 Mortgage Trust</name>
        <lei>N/A</lei>
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        <identifiers>
          <isin value="US12591RBC34"/>
          <ticker value="COMM"/>
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      <invstOrSec>
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          <isin value="US12592KBE38"/>
          <ticker value="COMM"/>
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      <invstOrSec>
        <name>COMM 2015-CCRE22 Mortgage Trust</name>
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        <title>COMM 2015-CCRE22 Mortgage Trust 3.60</title>
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      <invstOrSec>
        <name>COMM MORTGAGE TRUST COMM 2015 LC21 AM</name>
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        <title>COMM MORTGAGE TRUST COMM 2015 LC21 AM</title>
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      <invstOrSec>
        <name>CSAIL 2017-CX10 Commercial Mortgage Trust</name>
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        <title>CSAIL 2017-CX10 Commercial Mortgage Trust 3.67</title>
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      </invstOrSec>
      <invstOrSec>
        <name>CSMC Trust 2015-WIN1</name>
        <lei>N/A</lei>
        <title>CSMC Trust 2015-WIN1 3.50</title>
        <cusip>12649KAP2</cusip>
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          <isin value="US12649KAP21"/>
          <ticker value="CSMC"/>
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        <curCd>USD</curCd>
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        <assetCat>ABS-MBS</assetCat>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
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          <couponKind>Variable</couponKind>
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          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>COMM 2019-GC44 Mortgage Trust</name>
        <lei>N/A</lei>
        <title>COMM 2019-GC44 Mortgage Trust 3.26</title>
        <cusip>12655TBP3</cusip>
        <identifiers>
          <isin value="US12655TBP30"/>
          <ticker value="COMM"/>
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        <name>CHL Mortgage Pass-Through Trust 2004-5</name>
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        <isRestrictedSec>N</isRestrictedSec>

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      <invstOrSec>
        <name>Cendant Mort Capital LLC CDMC Mort Pas Thr Ce Se 03 6</name>
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        <name>Credit Agricole Corporate and Investment Bank/New York</name>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Fannie Mae Connecticut Avenue</name>
        <lei>N/A</lei>
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          <ticker value="CAS"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Freddie Mac Multifamily Structured Pass Through Certificates</name>
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        <title>Freddie Mac Multifamily Structured Pass Through Certificates 3.69</title>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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        <name>Freddie Mac Structured Agency</name>
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        <cusip>3137G0FJ3</cusip>
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          <ticker value="STACR"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Goldentree Loan Opportunities Ltd</name>
        <lei>549300MI5PVR6JHGVV87</lei>
        <title>Goldentree Loan Management US Clo 6 Ltd 3.66</title>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Hilton Grand Vacations Trust 2014-A</name>
        <lei>N/A</lei>
        <title>Hilton Grand Vacations Trust 2014-A 1.77</title>
        <cusip>43284AAA2</cusip>
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          <isin value="US43284AAA25"/>
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        <isRestrictedSec>N</isRestrictedSec>

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      <invstOrSec>
        <name>Hudson Yards 2016-10HY Mortgage Trust</name>
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        <title>Hudson Yards 2016-10HY Mortgage Trust 2.84</title>
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        <isRestrictedSec>N</isRestrictedSec>

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      <invstOrSec>
        <name>JP Morgan Chase Commercial Mortgage Securities Trust 2016-JP2</name>
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        <title>JP Morgan Chase Commercial Mortgage Securities Trust 2016-JP2 2.82</title>
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        <name>JP Morgan Chase Commercial Mortgage Securities Trust 2016-JP3</name>
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        <title>JP Morgan Chase Commercial Mortgage Securities Trust 2016-JP3 3.14</title>
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        <name>JPMDB Commercial Mortgage Securities Trust 2017-C5</name>
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        <name>MVW Owner Trust 2014-1</name>
        <lei>5493004KX4H48P1FVD32</lei>
        <title>MVW Owner Trust 2014-1 2.25</title>
        <cusip>553891AA0</cusip>
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        <name>MVW Owner Trust 2013-1</name>
        <lei>549300T62Z5TRF7JHO83</lei>
        <title>MVW Owner Trust 2013-1 2.15</title>
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      <invstOrSec>
        <name>Magnetite CLO Ltd</name>
        <lei>549300P6T1S83Z2MDF55</lei>
        <title>Magnetite XXIV Ltd 3.76</title>
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          <ticker value="MAGNE"/>
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        <issuerCat>CORP</issuerCat>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      </invstOrSec>
      <invstOrSec>
        <name>Millicom International Cellular SA</name>
        <lei>549300CTHC1CP86P2G96</lei>
        <title>Millicom International Cellular SA 5.13 01/15/2028</title>
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          <isin value="US600814AN71"/>
          <ticker value="TIGO"/>
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        <valUSD>3154072.5000000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>LU</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2028-01-15</maturityDt>
          <couponKind>Fixed</couponKind>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>Morgan Stanley Bank of America Merrill Lynch Trust 2017-C34</name>
        <lei>N/A</lei>
        <title>Morgan Stanley Bank of America Merrill Lynch Trust 2017-C34 3.28</title>
        <cusip>61767EAD6</cusip>
        <identifiers>
          <isin value="US61767EAD67"/>
          <ticker value="MSBAM"/>
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        <balance>5000000.0000000000</balance>
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        <curCd>USD</curCd>
        <valUSD>5250702.0000000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2052-11-15</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>3.276000000000000</annualizedRt>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Progress Residential 2018-SFR3 Trust</name>
        <lei>N/A</lei>
        <title>Progress Residential 2018-SFR3 Trust 3.88</title>
        <cusip>74331MAA4</cusip>
        <identifiers>
          <isin value="US74331MAA45"/>
          <ticker value="PROG"/>
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        <balance>11492774.7400000000</balance>
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        <curCd>USD</curCd>
        <valUSD>11721228.1200000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2035-10-17</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>3.880000000000000</annualizedRt>
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          <isCashCollateral>N</isCashCollateral>
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      <invstOrSec>
        <name>State Street Institutional US Government Money Market Fund</name>
        <lei>549300BZ5TGIFZUZDZ37</lei>
        <title>State Street Institutional US Government Money Market Fund</title>
        <cusip>857492706</cusip>
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          <isin value="US8574927062"/>
          <ticker value="GVMXX"/>
          <other otherDesc="SEDOL" value="B284MN4"/>
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        <balance>28702.4700000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>28702.4700000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Sequoia Mortgage Trust 2014-3</name>
        <lei>N/A</lei>
        <title>Sequoia Mortgage Trust 2014-3 3.00</title>
        <cusip>81746CBY7</cusip>
        <identifiers>
          <isin value="US81746CBY75"/>
          <ticker value="SEMT"/>
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        <balance>1298787.2200000000</balance>
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        <curCd>USD</curCd>
        <valUSD>1299832.2200000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2044-10-25</maturityDt>
          <couponKind>Variable</couponKind>
          <annualizedRt>3.000000000000000</annualizedRt>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Sequoia Mortgage Trust 2017-CH1</name>
        <lei>N/A</lei>
        <title>Sequoia Mortgage Trust 2017-CH1 4.00</title>
        <cusip>81746HAA9</cusip>
        <identifiers>
          <isin value="US81746HAA95"/>
          <ticker value="SEMT"/>
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        <balance>3161514.5100000000</balance>
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        <curCd>USD</curCd>
        <valUSD>3248351.8300000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2047-08-25</maturityDt>
          <couponKind>Variable</couponKind>
          <annualizedRt>4.000000000000000</annualizedRt>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      <invstOrSec>
        <name>Symphony CLO Ltd</name>
        <lei>N/A</lei>
        <title>Symphony CLO XIX Ltd 2.96</title>
        <cusip>87165YAC7</cusip>
        <identifiers>
          <isin value="US87165YAC75"/>
          <ticker value="SYMP"/>
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        <balance>5250000.0000000000</balance>
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        <curCd>USD</curCd>
        <valUSD>5206367.7800000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>ABS-CBDO</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2031-04-16</maturityDt>
          <couponKind>Variable</couponKind>
          <annualizedRt>2.960880000000000</annualizedRt>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>Thornburg Mortgage Securities Trust 2004-3</name>
        <lei>N/A</lei>
        <title>Thornburg Mortgage Securities Trust 2004-3 2.53</title>
        <cusip>885220FS7</cusip>
        <identifiers>
          <isin value="US885220FS76"/>
          <ticker value="TMST"/>
          <other otherDesc="SEDOL" value="B033CY3"/>
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        <balance>1068747.4400000000</balance>
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        <valUSD>1059018.1000000000</valUSD>
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        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2034-09-25</maturityDt>
          <couponKind>Variable</couponKind>
          <annualizedRt>2.532000000000000</annualizedRt>
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        <securityLending>
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      <invstOrSec>
        <name>Towd Point Mortgage Trust 2017-3</name>
        <lei>N/A</lei>
        <title>Towd Point Mortgage Trust 2017-3 3.50</title>
        <cusip>89169DAC5</cusip>
        <identifiers>
          <isin value="US89169DAC56"/>
          <ticker value="TPMT"/>
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        <balance>5000000.0000000000</balance>
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        <curCd>USD</curCd>
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        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
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          <couponKind>Variable</couponKind>
          <annualizedRt>3.500000000000000</annualizedRt>
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        <securityLending>
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      <invstOrSec>
        <name>Towd Point Mortgage Trust 2017-2</name>
        <lei>N/A</lei>
        <title>Towd Point Mortgage Trust 2017-2 3.25</title>
        <cusip>89173HAB2</cusip>
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          <isin value="US89173HAB24"/>
          <ticker value="TPMT"/>
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        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2057-04-25</maturityDt>
          <couponKind>Variable</couponKind>
          <annualizedRt>3.250000000000000</annualizedRt>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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      <invstOrSec>
        <name>Towd Point Mortgage Trust 2018-1</name>
        <lei>N/A</lei>
        <title>Towd Point Mortgage Trust 2018-1 3.00</title>
        <cusip>89176EAA8</cusip>
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          <isin value="US89176EAA82"/>
          <ticker value="TPMT"/>
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        <balance>3507630.5000000000</balance>
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        <issuerCat>CORP</issuerCat>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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          <couponKind>Variable</couponKind>
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      <invstOrSec>
        <name>Towd Point Mortgage Trust 2019-2</name>
        <lei>N/A</lei>
        <title>Towd Point Mortgage Trust 2019-2 3.75</title>
        <cusip>89177JAC2</cusip>
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          <isin value="US89177JAC27"/>
          <ticker value="TPMT"/>
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        <assetCat>ABS-MBS</assetCat>
        <issuerCat>CORP</issuerCat>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2058-12-25</maturityDt>
          <couponKind>Variable</couponKind>
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      <invstOrSec>
        <name>Towd Point Mortgage Trust 2019-4</name>
        <lei>N/A</lei>
        <title>Towd Point Mortgage Trust 2019-4 2.90</title>
        <cusip>89178BAA2</cusip>
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          <isin value="US89178BAA26"/>
          <ticker value="TPMT"/>
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        <balance>10340639.9700000000</balance>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Treman Park CLO</name>
        <lei>549300E4DX3Q8L455K94</lei>
        <title>Treman Park CLO Ltd 3.04</title>
        <cusip>89473LAH7</cusip>
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          <isin value="US89473LAH78"/>
          <ticker value="TRMPK"/>
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        <balance>4500000.0000000000</balance>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
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      <invstOrSec>
        <name>UBS Commercial Mortgage Trust 2017-C1</name>
        <lei>N/A</lei>
        <title>UBS Commercial Mortgage Trust 2017-C1 3.20</title>
        <cusip>90276EAD9</cusip>
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          <isin value="US90276EAD94"/>
          <ticker value="UBSCM"/>
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      <invstOrSec>
        <name>UBS Commercial Mortgage Trust</name>
        <lei>N/A</lei>
        <title>UBS Commercial Mortgage Trust 3.20</title>
        <cusip>90278MBD8</cusip>
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          <ticker value="UBSCM"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
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        <securityLending>
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      <invstOrSec>
        <name>United States Treasury Inflation Indexed Bonds</name>
        <lei>254900HROIFWPRGM1V77</lei>
        <title>United States Treasury Inflation Indexed Bonds 3.63 04/15/2028</title>
        <cusip>912810FD5</cusip>
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          <isin value="US912810FD55"/>
          <ticker value="TII"/>
          <other otherDesc="SEDOL" value="2235037"/>
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        <balance>48903434.8800000000</balance>
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        <curCd>USD</curCd>
        <valUSD>62573823.8000000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>DBT</assetCat>
        <issuerCat>UST</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2028-04-15</maturityDt>
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        <name>United States Treasury Inflation Indexed Bonds</name>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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        <name>WaMu Mortgage Pass-Through Certificates Series 2003-S11 Trust</name>
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        <isRestrictedSec>N</isRestrictedSec>

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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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        <name>Bank of America</name>
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        <name>Bank of America</name>
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      </invstOrSec>
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      </invstOrSec>
      <invstOrSec>
        <name>Barclays Bank PLC</name>
        <lei>G5GSEF7VJP5I7OUK5573</lei>
        <title>INFL 2.36 09/29/24</title>
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      <invstOrSec>
        <name>Barclays Bank PLC</name>
        <lei>G5GSEF7VJP5I7OUK5573</lei>
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      <invstOrSec>
        <name>Barclays Bank PLC</name>
        <lei>G5GSEF7VJP5I7OUK5573</lei>
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        <name>Bank of America</name>
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        <name>LCH.Clearnet Group Ltd</name>
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        <name>LCH.Clearnet Group Ltd</name>
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      <invstOrSec>
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        <fairValLevel>2</fairValLevel>
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        <name>LCH.Clearnet Group Ltd</name>
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      <invstOrSec>
        <name>LCH.Clearnet Group Ltd</name>
        <lei>WAM6YERMS7OXFZUOY219</lei>
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        <units>OU</units>
        <descOthUnits>Swap Contract Amount</descOthUnits>
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        <isRestrictedSec>N</isRestrictedSec>

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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
    </invstOrSecs>
    <signature>
      <ncom:dateSigned>2020-02-25</ncom:dateSigned>
      <ncom:nameOfApplicant>American Century Government Income Trust</ncom:nameOfApplicant>
      <ncom:signature>Amy R. Bitter</ncom:signature>
      <ncom:signerName>Amy R. Bitter</ncom:signerName>
      <ncom:title>Assistant Treasurer</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
