<?xml version="1.0" encoding="UTF-8"?>
<edgarSubmission xmlns:com="http://www.sec.gov/edgar/common" xmlns="http://www.sec.gov/edgar/ncen">
  <schemaVersion>X0201</schemaVersion>
  <headerData>
    <submissionType>N-CEN</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <filer>
        <issuerCredentials>
          <cik>0000769785</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>
      <flags>
        <returnCopyFlag>false</returnCopyFlag>
        <overrideInternetFlag>false</overrideInternetFlag>
        <confirmingCopyFlag>false</confirmingCopyFlag>
      </flags>
      <investmentCompanyType>N-6</investmentCompanyType>
    </filerInfo>
    <seriesClass>
      <reportClass>
        <classInfo>
          <classId>C000211638</classId>
        </classInfo>
      </reportClass>
    </seriesClass>
  </headerData>
  <formData>
    <generalInfo reportEndingPeriod="2018-12-31" isReportPeriodLt12="N"/>
    <registrantInfo>
      <registrantFullName>Separate Account One of Talcott Resolution Life Insurance Co</registrantFullName>
      <investmentCompFileNo>811-04315</investmentCompFileNo>
      <registrantCik>0000769785</registrantCik>
      <registrantLei>5493006PY1GB4H2HML66</registrantLei>
      <registrantstreet1>One Griffin Rd North</registrantstreet1>
      <registrantstreet2>Suite S4</registrantstreet2>
      <registrantcity>Windsor</registrantcity>
      <registrantzipCode>06095-1512</registrantzipCode>
      <registrantstate>US-CT</registrantstate>
      <registrantcountry>US</registrantcountry>
      <registrantphoneNumber>8605473194</registrantphoneNumber>
      <websites>
        <website webpage="www.policyowner-services.com"/>
      </websites>
      <locationBooksRecords>
        <locationBooksRecord>
          <officeName>Jackie Walding</officeName>
          <officeAddress1>751 Broad Street</officeAddress1>
          <officeCity>Newark</officeCity>
          <officeStateCountry officeState="US-NJ" officeCountry="US"/>
          <officeRecordsZipCode>07102</officeRecordsZipCode>
          <officePhone>9043131872</officePhone>
          <booksRecordsDesc>Records covering shareholder accounts and portfolio transactions</booksRecordsDesc>
        </locationBooksRecord>
      </locationBooksRecords>
      <isRegistrantFirstFiling>Y</isRegistrantFirstFiling>
      <isRegistrantLastFiling>N</isRegistrantLastFiling>
      <registrantFamilyInvComp isRegistrantFamilyInvComp="Y" familyInvCompFullName="Talcott"/>
      <registrantClassificationType>N-6</registrantClassificationType>
      <isSecuritiesActRegistration>Y</isSecuritiesActRegistration>
      <chiefComplianceOfficers>
        <chiefComplianceOfficer>
          <ccoName>Christopher Scott Conner</ccoName>
          <crdNumber>002239639</crdNumber>
          <ccoStreet1>1 Griffin Rd North</ccoStreet1>
          <ccoCity>Windsor</ccoCity>
          <ccoStateCountry ccoState="US-CT" ccoCountry="US"/>
          <ccoZipCode>06095</ccoZipCode>
          <ccoPhone>XXXXXX</ccoPhone>
          <isCcoChangedSinceLastFiling>Y</isCcoChangedSinceLastFiling>
          <ccoEmployers>
            <ccoEmployer ccoEmployerName="N/A" ccoEmployerId="N/A"/>
          </ccoEmployers>
        </chiefComplianceOfficer>
      </chiefComplianceOfficers>
      <isRegistrantSubmittedMatter>N</isRegistrantSubmittedMatter>
      <isPreviousLegalProceeding>N</isPreviousLegalProceeding>
      <isPreviousProceedingTerminated>N</isPreviousProceedingTerminated>
      <isFinancialSupportDuringPeriod>N</isFinancialSupportDuringPeriod>
      <isExemptionFromAct>N</isExemptionFromAct>
      <principalUnderwriters>
        <principalUnderwriter>
          <principalUnderwriterName>Pruco Securities, LLC</principalUnderwriterName>
          <principalUnderwriterFileNumber>8-16402</principalUnderwriterFileNumber>
          <principalUnderwriterCrdNumber>000005685</principalUnderwriterCrdNumber>
          <principalUnderwriterLei>N/A</principalUnderwriterLei>
          <principalUnderWriterStateCountry principalUnderWriterState="US-NJ" principalUnderWriterCountry="US"/>
          <isPrincipalUnderwriterAffiliatedWithRegistrant>Y</isPrincipalUnderwriterAffiliatedWithRegistrant>
        </principalUnderwriter>
      </principalUnderwriters>
      <isUnderwriterHiredOrTerminated>N</isUnderwriterHiredOrTerminated>
      <publicAccountants>
        <publicAccountant>
          <publicAccountantName>Deloitte &amp; Touche LLP</publicAccountantName>
          <pcaobNumber>00034</pcaobNumber>
          <publicAccountantLei>N/A</publicAccountantLei>
          <publicAccountantStateCountry publicAccountantState="US-CT" publicAccountantCountry="US"/>
        </publicAccountant>
      </publicAccountants>
      <isPublicAccountantChanged>N</isPublicAccountantChanged>
      <isOpinionOffered>N</isOpinionOffered>
      <isMaterialChange>N</isMaterialChange>
      <isAccountingPrincipleChange>N</isAccountingPrincipleChange>
    </registrantInfo>
    <unitInvestmentTrust>
      <depositors>
        <depositor>
          <depositorName>Talcott Resolution Life Insurance Company</depositorName>
          <depositorCrdNo>N/A</depositorCrdNo>
          <depositorLei>8CE7JPU9IU72L3PPD756</depositorLei>
          <depositorStateCountry depositorState="US-CT" depositorCountry="US"/>
          <depositorUltimateParentname>Talcott Resolution Life, Inc.</depositorUltimateParentname>
        </depositor>
      </depositors>
      <uitAdmins>
        <uitAdmin>
          <uitAdminName>The Prudential Insurance Company of America</uitAdminName>
          <uitAdminLei>X574KRZ6V5A7UBU45C31</uitAdminLei>
          <uitAdminStateCountry uitAdminState="US-NJ" uitAdminCountry="US"/>
          <isUitAdminAffiliated>N</isUitAdminAffiliated>
          <isUitAdminSubAdmin>N</isUitAdminSubAdmin>
        </uitAdmin>
        <uitAdmin>
          <uitAdminName>DXC Technology Company</uitAdminName>
          <uitAdminLei>549300DOVZ3EDJB7O259</uitAdminLei>
          <uitAdminStateCountry uitAdminState="US-NV" uitAdminCountry="US"/>
          <isUitAdminAffiliated>N</isUitAdminAffiliated>
          <isUitAdminSubAdmin>Y</isUitAdminSubAdmin>
        </uitAdmin>
      </uitAdmins>
      <isUitAdminHiredTerminated>N</isUitAdminHiredTerminated>
      <registrantSeparateInsuranceAccount isRegistrantSeparateInsuranceAccount="Y" separateAccountSeriesId="S000065444"/>
      <numOfContracts>111</numOfContracts>
      <contractSecurities>
        <contractSecurity separateAccountSecurityName="The Hartford's Builder" separateAccountContractId="C000211638" separateAccountTotalAsset="14872822.400000000000" numContractsSold="0" grossPremiumReceived="3146756.710000000000" grossPremiumReceivedSection1035="0.000000000000" numContractsAffected="0" contractValueRedeemed="17447973.000000000000" contractValueRedeemedSection1035="12631414.840000000000" numContractsAffectedRedeemed="2"/>
      </contractSecurities>
      <isRule6C7Reliance>N</isRule6C7Reliance>
      <isRule11A2Reliance>N</isRule11A2Reliance>
    </unitInvestmentTrust>
    <signature registrantSignedName="Separate Account One of Talcott Resolution Life Insurance Co" signedDate="2019-03-14" signature="Greg Steffe" title="VP Project Management"/>
  </formData>
</edgarSubmission>
