NPORT-P false 0000756913 XXXXXXXX S000008239 C000022443 C000022444 C000022442 John Hancock Variable Insurance Trust 811-04146 0000756913 Z5DUWKEPW78QKD87GA06 200 Berkeley Street Boston 02116 617-663-4497 Health Sciences Trust S000008239 K5HWWGQWUOYDF5BZA919 2021-12-31 2021-12-31 N 338708630.000000000000 74514.780000000000 338634115.220000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 0.000000000000 N Global Blood Therapeutics Inc. 549300U4G0QKK99GRT15 37890U108 6332 NS USD 185337.64 0.0547309416 Long EC CORP US N 1 N N N REGENXBIO Inc. 549300EOES47LATSET33 75901B107 4300 NS USD 140610 0.0415226918 Long EC CORP US N 1 N N N Relay Therapeutics Inc. N/A 75943R102 17071 NS USD 524250.41 0.1548132295 Long EC CORP US N 1 N N N Repligen Corporation 54930006QJ0T5AUO1E71 759916109 2555 NS USD 676666.2 0.1998222180 Long EC CORP US N 1 N N N Repare Therapeutics Inc. N/A 760273102 6100 NS USD 128649 0.0379905610 Long EC CORP CA N 1 N N N Royalty Pharma plc N/A 76028H209 30049 NS USD 1197452.65 0.3536125264 Long EC CORP US N 1 N N N Replimune Group Inc. N/A 76029N106 22880 NS USD 620048 0.1831026386 Long EC CORP US N 1 N N N ROWE T PRICE GOV RES FD 20340919 00000.0000000000 N/A 76105Y109 938339.44 NS USD 938339.44 0.2770953657 Long STIV RF US N 1 N N N Revolution Medicines Inc. N/A 76155X100 15300 NS USD 385101 0.1137218557 Long EC CORP US N 1 N N N Revolution Healthcare Acquisition Corp. N/A 76155Y207 31883 NS USD 314047.55 0.0927394896 Long EC CORP US N 1 N N N Rocket Pharmaceuticals Inc. 549300R7VV82YUIN4Y93 77313F106 8094 NS USD 176692.02 0.0521778557 Long EC CORP US N 1 N N N Rubius Therapeutics Inc. N/A 78116T103 12530 NS USD 121290.4 0.0358175371 Long EC CORP US N 1 N N N State Street Bank and Trust Company 571474TGEMMWANRLN572 7839989D1 626841.14 NS USD 626841.14 0.1851086798 Long STIV RF US N 1 N N N Sage Therapeutics Inc. 5493000JX4BJS9W6CN35 78667J108 7887 NS USD 335512.98 0.0990783164 Long EC CORP US N 1 N N N Sana Biotechnology Inc. N/A 799566104 7446 NS USD 115264.08 0.0340379409 Long EC CORP US N 1 N N N Sarepta Therapeutics Inc. N/A 803607100 4368 NS USD 393338.4 0.1161543927 Long EC CORP US N 1 N N N Scholar Rock Holding Corporation N/A 80706P103 17412 NS USD 432514.08 0.1277231267 Long EC CORP US N 1 N N N Schrodinger Inc. N/A 80810D103 4954 NS USD 172547.82 0.0509540570 Long EC CORP US N 1 N N N Seagen Inc. N/A 81181C104 17989 NS USD 2781099.4 0.8212697053 Long EC CORP US N 1 N N N Seer Inc. N/A 81578P106 46916 NS USD 1070153.96 0.3160207173 Long EC CORP US N 1 N N N Sema4 Holdings Corp N/A 81663L101 23688 NS USD 105648.48 0.0311984160 Long EC CORP US N 1 N N N Sema4 Holdings Corp N/A 81663L119 4862 NS USD 4764.76 0.0014070526 Long EC CORP US N 1 N N N ShockWave Medical Inc. N/A 82489T104 15493 NS USD 2762866.69 0.8158855135 Long EC CORP US N 1 N N N SomaLogic Inc. N/A 83444K105 67026 NS USD 780182.64 0.2303910341 Long EC CORP US N 1 N N N SomaLogic Inc. N/A 83444K113 2050 NS USD 6888 0.0020340538 Long EC CORP US N 1 N N N SpringWorks Therapeutics Inc. N/A 85205L107 5481 NS USD 339712.38 0.1003184159 Long EC CORP US N 1 N N N STERIS plc N/A 859152100 3763 NS USD 915951.83 0.2704842155 Long EC CORP US N 1 N N N Stoke Therapeutics Inc. N/A 86150R107 8627 NS USD 206961.73 0.0611166214 Long EC CORP US N 1 N N N Stryker Corporation 5493002F0SC4JTBU5137 863667101 29730 NS USD 7950396.6 2.3477837119 Long EC CORP US N 1 N N N Surgery Partners Inc. 549300LC82HG33S19I03 86881A100 16076 NS USD 858619.16 0.2535536502 Long EC CORP US N 1 N N N Teleflex Incorporated UUL85LWXB4NGSWWXIQ25 879369106 6914 NS USD 2271110.72 0.6706680213 Long EC CORP US N 1 N N N Tenaya Therapeutics Inc. N/A 87990A106 12198 NS USD 231152.1 0.0682601338 Long EC CORP US N 1 N N N 10x Genomics Inc. N/A 88025U109 9307 NS USD 1386370.72 0.4094007832 Long EC CORP US N 1 N N N Thermo Fisher Scientific Inc. HCHV7422L5HDJZCRFL38 883556102 24977 NS USD 16665653.48 4.9214337041 Long EC CORP US N 1 N N N Theseus Pharmaceuticals Inc. N/A 88369M101 16751 NS USD 212402.68 0.0627233555 Long EC CORP US N 1 N N N Turning Point Therapeutics Inc. N/A 90041T108 9894 NS USD 471943.8 0.1393668797 Long EC CORP US N 1 N N N Twist Bioscience Corporation N/A 90184D100 6477 NS USD 501255.03 0.1480226024 Long EC CORP US N 1 N N N Ultragenyx Pharmaceutical Inc. N/A 90400D108 27510 NS USD 2313315.9 0.6831313787 Long EC CORP US N 1 N N N Unitedhealth Group Incorporated 549300GHBMY8T5GXDE41 91324P102 49784 NS USD 24998537.76 7.3821675479 Long EC CORP US N 1 N N N Veeva Systems Inc. 549300YZEXPDB92M2F02 922475108 12043 NS USD 3076745.64 0.9085752149 Long EC CORP US N 1 N N N ACADIA Pharmaceuticals Inc. N/A 004225108 23708 NS USD 553344.72 0.1634048949 Long EC CORP US N 1 N N N Adagio Therapeutics Inc. N/A 00534A102 6100 NS USD 44286 0.0130778318 Long EC CORP US N 1 N N N Adaptive Biotechnologies Corporation N/A 00650F109 29813 NS USD 836552.78 0.2470373605 Long EC CORP US N 1 N N N Agilent Technologies Inc. QUIX8Y7A2WP0XRMW7G29 00846U101 38273 NS USD 6110284.45 1.8043912811 Long EC CORP US N 1 N N N Agios Pharmaceuticals Inc. N/A 00847X104 8938 NS USD 293792.06 0.0867579629 Long EC CORP US N 1 N N N Agiliti Inc. N/A 00848J104 31358 NS USD 726251.28 0.2144648892 Long EC CORP US N 1 N N N Agilon Health Inc. N/A 00857U107 16632 NS USD 449064 0.1326103838 Long EC CORP US N 1 N N N Akero Therapeutics Inc. N/A 00973Y108 4936 NS USD 104396.4 0.0308286718 Long EC CORP US N 1 N N N Alector Inc. N/A 014442107 11904 NS USD 245817.6 0.0725909142 Long EC CORP US N 1 N N N Alignment Healthcare Inc. N/A 01625V104 30079 NS USD 422910.74 0.1248872222 Long EC CORP US N 1 N N N Allakos Inc. N/A 01671P100 3100 NS USD 30349 0.0089621803 Long EC CORP US N 1 N N N Allogene Therapeutics Inc. N/A 019770106 18074 NS USD 269664.08 0.0796328745 Long EC CORP US N 1 N N N Alnylam Pharmaceuticals Inc. N/A 02043Q107 21834 NS USD 3702609.72 1.0933953650 Long EC CORP US N 1 N N N Amgen Inc. 62QBXGPJ34PQ72Z12S66 031162100 10308 NS USD 2318990.76 0.6848071874 Long EC CORP US N 1 N N N Annexon Inc. N/A 03589W102 16751 NS USD 192468.99 0.0568368576 Long EC CORP US N 1 N N N Anthem Inc. 8MYN82XMYQH89CTMTH67 036752103 14934 NS USD 6922506.36 2.0442436390 Long EC CORP US N 1 N N N Apellis Pharmaceuticals Inc. N/A 03753U106 21739 NS USD 1027819.92 0.3035193071 Long EC CORP US N 1 N N N Arcutis Biotherapeutics Inc. N/A 03969K108 5800 NS USD 120292 0.0355227057 Long EC CORP US N 1 N N N argenx SE N/A 04016X101 14579 NS USD 5105420.01 1.5076508185 Long EC CORP NL N 1 N N N Arvinas Inc. N/A 04335A105 11123 NS USD 913643.22 0.2698024738 Long EC CORP US N 1 N N N Ascendis Pharma A/S N/A 04351P101 14965 NS USD 2013241.45 0.5945182011 Long EC CORP DK N 1 N N N AstraZeneca PLC PY6ZZQWO2IZFZC3IOL08 046353108 125782 NS USD 7326801.5 2.1636336006 Long EC CORP GB N 1 N N N Atea Pharmaceuticals Inc. N/A 04683R106 4587 NS USD 41007.78 0.0121097604 Long EC CORP US N 1 N N N AtriCure Inc. N/A 04963C209 5862 NS USD 407584.86 0.1203614290 Long EC CORP US N 1 N N N Avantor Inc. N/A 05352A100 80712 NS USD 3401203.68 1.0043889635 Long EC CORP US N 1 N N N Avidity Biosciences Inc. N/A 05370A108 20231 NS USD 480890.87 0.1420089850 Long EC CORP US N 1 N N N Becton Dickinson and Company ICE2EP6D98PQUILVRZ91 075887109 19629 NS USD 4936300.92 1.4577092792 Long EC CORP US N 1 N N N BeiGene Ltd. 549300JFUK6FRD5MH739 07725L102 4485 NS USD 1215121.05 0.3588300751 Long EC CORP CN N 1 N N N Berkeley Lights Inc. N/A 084310101 6931 NS USD 126005.58 0.0372099485 Long EC CORP US N 1 N N N Bicycle Therapeutics PLC N/A 088786108 6391 NS USD 389020.17 0.1148792022 Long EC CORP GB N 1 N N N Biogen Inc. W8J5WZB5IY3K0NDQT671 09062X103 6345 NS USD 1522292.4 0.4495389955 Long EC CORP US N 1 N N N Bio-Techne Corporation N/A 09073M104 2300 NS USD 1189882 0.3513768833 Long EC CORP US N 1 N N N BioNTech SE N/A 09075V102 20380 NS USD 5253964 1.5515164491 Long EC CORP DE N 1 N N N Blueprint Medicines Corporation N/A 09627Y109 13364 NS USD 1431418.04 0.4227034359 Long EC CORP US N 1 N N N Bruker Corporation LEBZ6VLQ6I8XB1NHVY15 116794108 37100 NS USD 3113061 0.9192992850 Long EC CORP US N 1 N N N Burning Rock Biotech Limited N/A 12233L107 9445 NS USD 90010.85 0.0265805617 Long EC CORP CN N 1 N N N C4 Therapeutics Inc. N/A 12529R107 22317 NS USD 718607.4 0.2122076211 Long EC CORP US N 1 N N N Cigna Corporation N/A 125523100 14966 NS USD 3436642.58 1.0148542115 Long EC CORP US N 1 N N N Catalent Inc. N/A 148806102 14481 NS USD 1854002.43 0.5474942856 Long EC CORP US N 1 N N N Celldex Therapeutics Inc. N/A 15117B202 13022 NS USD 503170.08 0.1485881243 Long EC CORP US N 1 N N N Centene Corporation 549300Z7JJ4TQSQGT333 15135B101 75925 NS USD 6256220 1.8474866290 Long EC CORP US N 1 N N N Centessa Pharmaceuticals PLC N/A 152309100 18009 NS USD 202781.34 0.0598821356 Long EC CORP US N 1 N N N Cerevel Therapeutics Holdings Inc. N/A 15678U128 29170 NS USD 945691.4 0.2792664287 Long EC CORP US N 1 N N N Certara Inc. N/A 15687V109 17771 NS USD 505051.82 0.1491438096 Long EC CORP US N 1 N N N The Cooper Companies Inc. QJKMVPIGLH7530PCUE20 216648402 3546 NS USD 1485561.24 0.4386921380 Long EC CORP US N 1 N N N Cytokinetics Incorporated 549300BE5DY1HW7IHA64 23282W605 1600 NS USD 72928 0.0215359282 Long EC CORP US N 1 N N N DICE Therapeutics Inc. N/A 23345J104 9328 NS USD 236091.68 0.0697188114 Long EC CORP US N 1 N N N Danaher Corporation S4BKK9OTCEWQ3YHPFM11 235851102 42335 NS USD 13928638.35 4.1131822590 Long EC CORP US N 1 N N N Day One Biopharmaceuticals Inc. N/A 23954D109 8112 NS USD 136687.2 0.0403642734 Long EC CORP US N 1 N N N Definitive Healthcare Corp. N/A 24477E103 11013 NS USD 300985.29 0.0888821523 Long EC CORP US N 1 N N N Denali Therapeutics Inc. N/A 24823R105 25147 NS USD 1121556.2 0.3312000031 Long EC CORP US N 1 N N N Design Therapeutics Inc. N/A 25056L103 13731 NS USD 293980.71 0.0868136720 Long EC CORP US N 1 N N N Dexcom Inc. N/A 252131107 4926 NS USD 2645015.7 0.7810836478 Long EC CORP US N 1 N N N Doximity Inc. N/A 26622P107 54693 NS USD 2741760.09 0.8096526507 Long EC CORP US N 1 N N N Dynamics Special Purpose Corp. N/A 268010105 18789 NS USD 185259.54 0.0547078784 Long EC CORP US N 1 N N N EQRx Inc. N/A 26886C107 22514 NS USD 153545.48 0.0453425905 Long EC CORP US N 1 N N N EQRx Inc. N/A 26886C115 4502 NS USD 4682.08 0.0013826368 Long EC CORP US N 1 N N N Elanco Animal Health Incorporated N/A 28414H103 19393 NS USD 550373.34 0.1625274346 Long EC CORP US N 1 N N N Enanta Pharmaceuticals Inc. N/A 29251M106 4200 NS USD 314076 0.0927478910 Long EC CORP US N 1 N N N Entrada Therapeutics Inc. N/A 29384C108 11328 NS USD 193935.36 0.0572698825 Long EC CORP US N 1 N N N Exact Sciences Corporation N/A 30063P105 21357 NS USD 1662215.31 0.4908587869 Long EC CORP US N 1 N N N Exelixis Inc. N/A 30161Q104 72356 NS USD 1322667.68 0.3905890224 Long EC CORP US N 1 N N N Exscientia plc N/A 30223G102 4147 NS USD 81944.72 0.0241986015 Long EC CORP GB N 1 N N N F-star Therapeutics Inc. N/A 30315R107 12596 NS USD 63231.92 0.0186726373 Long EC CORP US N 1 N N N Fate Therapeutics Inc. N/A 31189P102 20672 NS USD 1209518.72 0.3571756848 Long EC CORP US N 1 N N N Generation Bio Co. N/A 37148K100 31607 NS USD 223777.56 0.0660824028 Long EC CORP US N 1 N N N Ginkgo Bioworks Holdings Inc. N/A 37611X100 104300 NS USD 866733 0.2559496994 Long EC CORP US N 1 N N N Prothena Corporation Public Limited Company N/A G72800108 16560 NS USD 818064 0.2415775503 Long EC CORP IE N 1 N N N ADC Therapeutics SA N/A H0036K147 19326 NS USD 390385.2 0.1152823010 Long EC CORP CH N 1 N N N CRISPR Therapeutics AG N/A H17182108 4552 NS USD 344950.56 0.1018652712 Long EC CORP CH N 1 N N N SOPHiA GENETICS SA N/A H82027105 20504 NS USD 289106.4 0.0853742689 Long EC CORP CH N 1 N N N CureVac N.V. N/A N2451R105 7486 NS USD 256844.66 0.0758472488 Long EC CORP DE N 1 N N N uniQure N.V. N/A N90064101 10600 NS USD 219844 0.0649208069 Long EC CORP NL N 1 N N N Ortho Clinical Diagnostics Holdings plc N/A G6829J107 67405 NS USD 1441792.95 0.4257671880 Long EC CORP US N 1 N N N Eucrates Biomedical Acquisition Corp. N/A G3141W114 11914 NS USD 118901.72 0.0351121504 Long EC CORP US N 1 N N N Frontier Acquisition Corp. N/A G36826116 12607 NS USD 124557.16 0.0367822244 Long EC CORP US N 1 N N N Health Sciences Acquisitions Corporation 2 N/A G4411D109 13354 NS USD 133540 0.0394348927 Long EC CORP US N 1 N N N Helix Acquisition Corp. N/A G4444C102 12247 NS USD 121122.83 0.0357680531 Long EC CORP US N 1 N N N MeiraGTx Holdings plc N/A G59665102 10086 NS USD 239441.64 0.0707080679 Long EC CORP US N 1 N N N NovoCure Limited N/A G6674U108 10720 NS USD 804857.6 0.2376776479 Long EC CORP JE N 1 N N N Merck and Co. Inc. 4YV9Y5M8S0BRK1RP0397 58933Y105 73030 NS USD 5597019.2 1.6528220130 Long EC CORP US N 1 N N N Mettler-Toledo International Inc. 5493000BD5GJNUDIUG10 592688105 883 NS USD 1498636.43 0.4425532936 Long EC CORP US N 1 N N N Mirati Therapeutics Inc. N/A 60468T105 12786 NS USD 1875578.34 0.5538657376 Long EC CORP US N 1 N N N Moderna Inc. N/A 60770K107 20712 NS USD 5260433.76 1.5534269950 Long EC CORP US N 1 N N N Molina Healthcare Inc. 549300NQQCEQ46YHZ591 60855R100 12174 NS USD 3872305.92 1.1435073272 Long EC CORP US N 1 N N N Monte Rosa Therapeutics Inc. N/A 61225M102 21606 NS USD 441194.52 0.1302864951 Long EC CORP US N 1 N N N Morphic Holding Inc. N/A 61775R105 4583 NS USD 217142.54 0.0641230550 Long EC CORP US N 1 N N N MultiPlan Corporation N/A 62548M100 35600 NS USD 157708 0.0465717992 Long EC CORP US N 1 N N N Eisai Co. Ltd. N/A 630720001 6800 NS 386021.08 0.1139935590 Long EC CORP JP N 2 N N N Neurocrine Biosciences Inc. 549300FECER0XBN49756 64125C109 6765 NS USD 576175.05 0.1701467821 Long EC CORP US N 1 N N N Nevro Corp. N/A 64157F103 4319 NS USD 350141.33 0.1033981263 Long EC CORP US N 1 N N N Novavax Inc. N/A 670002401 2476 NS USD 354241.32 0.1046088696 Long EC CORP US N 1 N N N Nurix Therapeutics Inc. N/A 67080M103 15120 NS USD 437724 0.1292616368 Long EC CORP US N 1 N N N Oak Street Health Inc. N/A 67181A107 7898 NS USD 261739.72 0.0772927795 Long EC CORP US N 1 N N N Olink Holding AB N/A 680710100 29231 NS USD 532004.2 0.1571029545 Long EC CORP SE N 1 N N N Option Care Health Inc. 549300DI3Q5ACSYJRI17 68404L201 28246 NS USD 803316.24 0.2372224782 Long EC CORP US N 1 N N N Orion Acquisition Corp. N/A 68626A207 12953 NS USD 127587.05 0.0376769629 Long EC CORP US N 1 N N N Outset Medical Inc. N/A 690145107 11304 NS USD 521001.36 0.1538537721 Long EC CORP US N 1 N N N PTC Therapeutics Inc. 549300UJLWOIWFDGB318 69366J200 9074 NS USD 361417.42 0.1067280004 Long EC CORP US N 1 N N N Pacific Biosciences of California Inc. N/A 69404D108 54894 NS USD 1123131.24 0.3316651186 Long EC CORP US N 1 N N N Pardes Biosciences Inc. N/A 69945Q105 16438 NS USD 269090.06 0.0794633641 Long EC CORP US N 1 N N N Penumbra Inc. 549300RGE06P3ECANU72 70975L107 10080 NS USD 2896185.6 0.8552551175 Long EC CORP US N 1 N N N Roche Holding AG N/A 711038901 7704 NS 3196090.13 0.9438181171 Long EC CORP CH N 2 N N N Pfizer Inc. 765LHXWGK1KXCLTFYQ30 717081103 226298 NS USD 13362896.9 3.9461165604 Long EC CORP US N 1 N N N Phreesia Inc. N/A 71944F106 21520 NS USD 896523.2 0.2647468639 Long EC CORP US N 1 N N N Lonza Group AG 549300EFW4H2TCZ71055 733337901 1486 NS 1237225.68 0.3653576602 Long EC CORP CH N 2 N N N Prelude Therapeutics Incorporated N/A 74065P101 3938 NS USD 49028.1 0.0144781928 Long EC CORP US N 1 N N N PROCEPT BioRobotics Corporation N/A 74276L105 9903 NS USD 247674.03 0.0731391253 Long EC CORP US N 1 N N N Protagonist Therapeutics Inc. N/A 74366E102 8019 NS USD 274249.8 0.0809870558 Long EC CORP US N 1 N N N PROGENIC PHARMACEUTICALS INC. COMMON STOCK N/A 743CVR037 21900 NS USD 935.13 0.0002761476 Long EC CORP US N 3 N N N Quantum-Si Incorporated N/A 74765K105 31200 NS USD 245544 0.0725101190 Long EC CORP US N 1 N N N Quanterix Corporation N/A 74766Q101 10589 NS USD 448973.6 0.1325836884 Long EC CORP US N 1 N N N Quidel Corporation 549300X2FQ6S98OQLQ46 74838J101 8384 NS USD 1131756.16 0.3342120918 Long EC CORP US N 1 N N N Rapid Micro Biosystems Inc. N/A 75340L104 10992 NS USD 116954.88 0.0345372409 Long EC CORP US N 1 N N N RAPT Therapeutics Inc. N/A 75382E109 13742 NS USD 504743.66 0.1490528087 Long EC CORP US N 1 N N N Reata Pharmaceuticals Inc. N/A 75615P103 2957 NS USD 77976.09 0.0230266493 Long EC CORP US N 1 N N N Recursion Pharmaceuticals Inc. N/A 75629V104 10414 NS USD 178391.82 0.0526798134 Long EC CORP US N 1 N N N Iovance Biotherapeutics Inc. 549300220Z2GHH33XL82 462260100 28633 NS USD 546603.97 0.1614143246 Long EC CORP US N 1 N N N IVERIC bio Inc. 549300GZXI59U71XPM70 46583P102 46124 NS USD 771193.28 0.2277364404 Long EC CORP US N 1 N N N Merck KGaA N/A 474184900 7966 NS 2049309.68 0.6051692927 Long EC CORP DE N 2 N N N Karuna Therapeutics Inc. N/A 48576A100 7771 NS USD 1018001 0.3006197410 Long EC CORP US N 1 N N N Kodiak Sciences Inc. N/A 50015M109 24412 NS USD 2069649.36 0.6111756811 Long EC CORP US N 1 N N N Kronos Bio Inc. N/A 50107A104 7495 NS USD 101857.05 0.0300787917 Long EC CORP US N 1 N N N Kymera Therapeutics Inc. N/A 501575104 13525 NS USD 858702.25 0.2535781870 Long EC CORP US N 1 N N N Lantheus Holdings Inc. N/A 516544103 18533 NS USD 535418.37 0.1581111725 Long EC CORP US N 1 N N N LIANBIO ADR USD.0001 N/A 53000N108 30782 NS USD 189617.12 0.0559946891 Long EC CORP KY N 1 N N N Eli Lilly and Company FRDRIPF3EKNDJ2CQJL29 532457108 36675 NS USD 10130368.5 2.9915380774 Long EC CORP US N 1 N N N Longboard Pharmaceuticals Inc. N/A 54300N103 8722 NS USD 42214.48 0.0124661037 Long EC CORP US N 1 N N N Lyell Immunopharma Inc. N/A 55083R104 32649 NS USD 252703.26 0.0746242770 Long EC CORP US N 1 N N N Maravai LifeSciences Holdings Inc. N/A 56600D107 16748 NS USD 701741.2 0.2072269652 Long EC CORP US N 1 N N N MaxCyte Inc. N/A 57777K106 7547 NS USD 76903.93 0.0227100362 Long EC CORP US N 1 N N N MaxCyte Inc. N/A 57777K106 11231 NS 114335.11 0.0337636124 Long EC CORP US N 2 N N N Evotec SE N/A 581191905 16612 NS 801924.9 0.2368116099 Long EC CORP DE N 2 N N N Sartorius AG N/A 584332902 3704 NS 2505163.4 0.7397847079 Long EP CORP DE N 2 N N N Regeneron Pharmaceuticals Inc. 549300RCBFWIRX3HYQ56 75886F107 7374 NS USD 4656828.48 1.3751799570 Long EC CORP US N 1 N N N Vir Biotechnology Inc. N/A 92764N102 7100 NS USD 297277 0.0877870795 Long EC CORP US N 1 N N N Warby Parker Inc. N/A 93403J106 48065 NS USD 2237906.4 0.6608626536 Long EC CORP US N 1 N N N Waters Corporation 5T547R1474YC9HOD8Q74 941848103 2305 NS USD 858843 0.2536197511 Long EC CORP US N 1 N N N ACERTA FUTURE PAYMENTS PP TC30M7746 N/A N/A 679276.72 NS USD 611349.05 0.1805338040 Long EC CORP US Y 3 N N N West Pharmaceutical Services Inc. 5493007NKRGM1RJQ1P16 955306105 13215 NS USD 6197967.15 1.8302843309 Long EC CORP US N 1 N N N Wuxi Biologics (Cayman) Inc. N/A 967JXD907 57500 NS 680736.4 0.2010241643 Long EC CORP CN N 2 N N N Xencor Inc. 549300V5IF65437JKG30 98401F105 17555 NS USD 704306.6 0.2079845380 Long EC CORP US N 1 N N N Zai Lab Limited N/A 98887Q104 8301 NS USD 521717.85 0.1540653545 Long EC CORP CN N 1 N N N Zentalis Pharmaceuticals Inc. N/A 98943L107 18642 NS USD 1567046.52 0.4627550650 Long EC CORP US N 1 N N N Zimmer Biomet Holdings Inc. 2P2YLDVPES3BXQ1FRB91 98956P102 8131 NS USD 1032962.24 0.3050378546 Long EC CORP US N 1 N N N Zymeworks Inc. N/A 98985W102 8723 NS USD 142969.97 0.0422196003 Long EC CORP CA N 1 N N N Alcon Inc. N/A ACI198PY5 10044 NS 885934.51 0.2616199816 Long EC CORP CH N 2 N N N Daiichi Sankyo Company Limited N/A B0J7D9901 25000 NS 636285.4 0.1878976073 Long EC CORP JP N 2 N N N Abcam plc N/A B67746907 26439 NS 618218.54 0.1825623917 Long EC CORP GB N 2 N N N Alkermes Public Limited Company 549300YHRMYLL071E243 G01767105 3287 NS USD 76455.62 0.0225776484 Long EC CORP IE N 1 N N N BCLS Acquisition Corp. N/A G0904B105 12029 NS USD 118605.94 0.0350248054 Long EC CORP US N 1 N N N Aadi Bioscience Inc. N/A 00032Q104 3700 NS USD 89355 0.0263868866 Long EC CORP US N 1 N N N AbbVie Inc. FR5LCKFTG8054YNNRU85 00287Y109 46643 NS USD 6315462.2 1.8649810861 Long EC CORP US N 1 N N N Guardant Health Inc. N/A 40131M109 12694 NS USD 1269653.88 0.3749338365 Long EC CORP US N 1 N N N HCA Healthcare Inc. N/A 40412C101 19041 NS USD 4892013.72 1.4446310930 Long EC CORP US N 1 N N N Hologic Inc. 549300DYP6F5ZJL0LB74 436440101 51507 NS USD 3943375.92 1.1644945807 Long EC CORP US N 1 N N N Humana Inc. N/A 444859102 14822 NS USD 6875332.92 2.0303131347 Long EC CORP US N 1 N N N IGM Biosciences Inc. N/A 449585108 6866 NS USD 201379.78 0.0594682493 Long EC CORP US N 1 N N N iRhythm Technologies Inc. 549300FJI72RJGDQS384 450056106 7605 NS USD 895032.45 0.2643066395 Long EC CORP US N 1 N N N IDEAYA Biosciences Inc. N/A 45166A102 14847 NS USD 350983.08 0.1036466984 Long EC CORP US N 1 N N N Imago BioSciences Inc. N/A 45250K107 12479 NS USD 295877.09 0.0873736805 Long EC CORP US N 1 N N N Immuneering Corporation N/A 45254E107 23271 NS USD 376292.07 0.1111205437 Long EC CORP US N 1 N N N Immunocore Holdings PLC N/A 45258D105 5762 NS USD 197290.88 0.0582607809 Long EC CORP GB N 1 N N N Inari Medical Inc. N/A 45332Y109 3430 NS USD 313056.1 0.0924467105 Long EC CORP US N 1 N N N Incyte Corporation 549300Z4WN6JVZ3T4680 45337C102 37491 NS USD 2751839.4 0.8126291110 Long EC CORP US N 1 N N N Insmed Incorporated N/A 457669307 42782 NS USD 1165381.68 0.3441418415 Long EC CORP US N 1 N N N Insulet Corporation 549300TZTYD2PYN92D43 45784P101 5579 NS USD 1484404.53 0.4383505569 Long EC CORP US N 1 N N N Intellia Therapeutics Inc. 5493002T5BCPE5DZC723 45826J105 7017 NS USD 829690.08 0.2450107779 Long EC CORP US N 1 N N N Genmab A/S N/A 459573903 3987 NS 1591422.99 0.4699535335 Long EC CORP DK N 2 N N N Intuitive Surgical Inc. 54930052SRG011710797 46120E602 38376 NS USD 13788496.8 4.0717979023 Long EC CORP US N 1 N N N Invitae Corporation 549300MYI59DZW1WIX92 46185L103 12344 NS USD 188492.88 0.0556626966 Long EC CORP US N 1 N N N Ionis Pharmaceuticals Inc. 549300SI4ZGLG0BLUZ92 462222100 24058 NS USD 732084.94 0.2161875922 Long EC CORP US N 1 N N N Vertex Pharmaceuticals Incorporated 54930015RAQRRZ5ZGJ91 92532F100 23912 NS USD 5251075.2 1.5506633750 Long EC CORP US N 1 N N N 2022-01-28 John Hancock Variable Insurance Trust /s/Salvatore Schiavone Salvatore Schiavone Treasurer