CONDENSED CONSOLIDATED STATEMENTS OF OPERATIONS - USD ($)	3 Months Ended		9 Months Ended
	Jun. 30, 2025	Jun. 30, 2024	Jun. 30, 2025	Jun. 30, 2024
Revenues
Total revenues	$ 304,393	$ 472,789	$ 1,937,506	$ 1,625,863
Cost of product revenues	299,263	230,188	930,619	853,034
Gross profit	5,130	242,601	1,006,887	772,829
Operating expenses:
Selling, general and administrative	2,930,627	2,635,863	8,423,602	8,440,919
Research and development	768,563	913,031	2,632,931	2,762,040
Total operating expenses	3,699,190	3,548,894	11,056,533	11,202,959
LOSS FROM OPERATIONS	(3,694,060)	(3,306,293)	(10,049,646)	(10,430,130)
Interest income	40,267	29,688	168,762	33,989
Transaction costs allocated to warrant liabilities				(633,198)
Unrealized gain on change in fair value of warrants classified as a liability	6,410	5,160,000	318,840	9,564,000
Unrealized loss on change in fair value of warrants classified as a liability - warrant modification				(394,000)
Loss on issuance of warrants				(1,633,767)
Other expense, net	(531)	(103)	(23,778)	(9,060)
(Loss) income before provision for income taxes	(3,647,914)	1,883,292	(9,585,822)	(3,502,166)
Net loss from continuing operations	(3,647,914)	1,883,292	(9,585,822)	(3,502,166)
Net loss from discontinued operations, net of tax	(336,195)	(33,791)	(403,120)	(272,397)
NET (LOSS) INCOME	(3,984,109)	1,849,501	(9,988,942)	(3,774,563)
Less: Net loss attributable to noncontrolling interest	38,746	30,295	99,992	78,785
NET (LOSS) INCOME attributable to Applied DNA Sciences, Inc.	(3,945,363)	1,879,796	(9,888,950)	(3,695,778)
Deemed dividend related to warrant modifications	(15,500,244)		(54,326,896)	(233,087)
NET (LOSS) INCOME attributable to common stockholders	$ (19,445,607)	$ 1,879,796	$ (64,215,846)	$ (3,928,865)
Net (loss) income per share attributable to common stockholders - basic (in dollars per share)	$ (33.41)	$ 1,191.52	$ (255.14)	$ (4,862.32)
Net (loss) income per share attributable to common stockholders - diluted (in dollars per share)	(33.41)	1,191.52	(255.14)	(4,862.32)
Net loss per share attributable to common stockholders-discontinued operations - basic (in dollars per share)	(0.59)	(21.04)	(1.61)	(362.23)
Net loss per share attributable to common stockholders-discontinued operations - diluted (in dollars per share)	(0.59)	(21.04)	(1.61)	(362.23)
Net (loss) income per share attributable to common stockholders - basic (in dollars per share)	(34)	1,170.48	(256.75)	(5,224.55)
Net (loss) income per share attributable to common stockholders - diluted (in dollars per share)	$ (34)	$ 1,170.48	$ (256.75)	$ (5,224.55)
Weighted average shares outstanding- basic (in shares)	572,018	1,606	250,107	752
Weighted average shares outstanding- diluted (in shares)	572,018	1,606	250,107	752
Product revenues
Revenues
Total revenues	$ 195,262	$ 246,644	$ 1,239,747	$ 947,086
Service revenues
Revenues
Total revenues	$ 109,131	$ 226,145	$ 697,759	$ 678,777