<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3/A</documentType>

    <periodOfReport>2019-01-12</periodOfReport>

    <dateOfOriginalSubmission>2019-01-16</dateOfOriginalSubmission>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0000720154</issuerCik>
        <issuerName>Inotiv, Inc.</issuerName>
        <issuerTradingSymbol>NOTV</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001762245</rptOwnerCik>
            <rptOwnerName>Leasure Robert Jr.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>2701 KENT AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>WEST LAFAYETTE</rptOwnerCity>
            <rptOwnerState>IN</rptOwnerState>
            <rptOwnerZipCode>47906-1382</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>President and CEO</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>26100</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnote</value>
                    <footnoteId id="F1"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">These shares are held by an entity for which the reporting person is the majority security holder.  Except to the extent of his pecuniary interest therein, the reporting person disclaims beneficial ownership of these shares.</footnote>
    </footnotes>

    <remarks>The reporting person's Form 3 filed on January 16, 2019 (and subsequent Form 4s) reported that 26,100 shares were owned directly by the reporting person. However, those shares are held indirectly by the reporting person.  This Form 3/A is being filed to correctly reflect that the 26,100 shares were owned indirectly by the reporting person.</remarks>

    <ownerSignature>
        <signatureName>/s/ Beth Taylor, Attorney-in-Fact for Robert Leasure, Jr.</signatureName>
        <signatureDate>2024-01-25</signatureDate>
    </ownerSignature>
</ownershipDocument>
