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<!--Created: Thu Mar 07 19:29:51 UTC 2024-->
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    <generalInfo reportEndingPeriod="2023-12-31" isReportPeriodLt12="N"/>
    <registrantInfo>
      <registrantFullName>Metropolitan Tower Separate Account One</registrantFullName>
      <investmentCompFileNo>811-03617</investmentCompFileNo>
      <registrantCik>0000710828</registrantCik>
      <registrantLei>549300ELU2IGIUHZYB71</registrantLei>
      <registrantstreet1>C/O Metropolitan Tower Life Insurance Co</registrantstreet1>
      <registrantstreet2>200 Park Avenue</registrantstreet2>
      <registrantcity>New York</registrantcity>
      <registrantzipCode>10166</registrantzipCode>
      <registrantstate>US-NY</registrantstate>
      <registrantcountry>US</registrantcountry>
      <registrantphoneNumber>212-578-9071</registrantphoneNumber>
      <websites>
        <website webpage="N/A"/>
      </websites>
      <locationBooksRecords>
        <locationBooksRecord>
          <officeName>Robin Wagner</officeName>
          <officeAddress1>200 Park Avenue</officeAddress1>
          <officeCity>New York</officeCity>
          <officeStateCountry officeState="US-NY" officeCountry="US"/>
          <officeRecordsZipCode>10166</officeRecordsZipCode>
          <officePhone>212-578-9071</officePhone>
          <booksRecordsDesc>paper/electronic</booksRecordsDesc>
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      </locationBooksRecords>
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      <isRegistrantLastFiling>N</isRegistrantLastFiling>
      <isRegistrantFamilyInvComp>N</isRegistrantFamilyInvComp>
      <registrantClassificationType>N-6</registrantClassificationType>
      <isSecuritiesActRegistration>Y</isSecuritiesActRegistration>
      <chiefComplianceOfficers>
        <chiefComplianceOfficer>
          <ccoName>Alexis Kuchinsky</ccoName>
          <crdNumber>004343809</crdNumber>
          <ccoStreet1>One MetLife Way</ccoStreet1>
          <ccoCity>Whippany</ccoCity>
          <ccoStateCountry ccoState="US-NJ" ccoCountry="US"/>
          <ccoZipCode>07981</ccoZipCode>
          <ccoPhone>XXXXXX</ccoPhone>
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        </chiefComplianceOfficer>
      </chiefComplianceOfficers>
      <isRegistrantSubmittedMatter>N</isRegistrantSubmittedMatter>
      <isPreviousLegalProceeding>N</isPreviousLegalProceeding>
      <isPreviousProceedingTerminated>N</isPreviousProceedingTerminated>
      <isFinancialSupportDuringPeriod>N</isFinancialSupportDuringPeriod>
      <releaseNumbers isExemptionFromAct="Y">
        <releaseNumberInfo releaseNumber="IC-13351"/>
        <releaseNumberInfo releaseNumber="IC-13580"/>
        <releaseNumberInfo releaseNumber="IC-14747"/>
        <releaseNumberInfo releaseNumber="IC-20235"/>
      </releaseNumbers>
      <principalUnderwriters>
        <principalUnderwriter>
          <principalUnderwriterName>MetLife Investors Distribution Company</principalUnderwriterName>
          <principalUnderwriterFileNumber>008-53064</principalUnderwriterFileNumber>
          <principalUnderwriterCrdNumber>000107622</principalUnderwriterCrdNumber>
          <principalUnderwriterLei>N/A</principalUnderwriterLei>
          <principalUnderWriterStateCountry principalUnderWriterState="US-MO" principalUnderWriterCountry="US"/>
          <isPrincipalUnderwriterAffiliatedWithRegistrant>Y</isPrincipalUnderwriterAffiliatedWithRegistrant>
        </principalUnderwriter>
      </principalUnderwriters>
      <isUnderwriterHiredOrTerminated>N</isUnderwriterHiredOrTerminated>
      <publicAccountants>
        <publicAccountant>
          <publicAccountantName>Deloitte &amp; Touche, LLP</publicAccountantName>
          <pcaobNumber>34</pcaobNumber>
          <publicAccountantLei>N/A</publicAccountantLei>
          <publicAccountantStateCountry publicAccountantState="US-NY" publicAccountantCountry="US"/>
        </publicAccountant>
      </publicAccountants>
      <isPublicAccountantChanged>N</isPublicAccountantChanged>
      <isOpinionOffered>N</isOpinionOffered>
      <isMaterialChange>N</isMaterialChange>
      <isAccountingPrincipleChange>N</isAccountingPrincipleChange>
    </registrantInfo>
    <unitInvestmentTrust>
      <depositors>
        <depositor>
          <depositorName>Metropolitan Tower Life Insurance Company</depositorName>
          <depositorCrdNo>N/A</depositorCrdNo>
          <depositorLei>C66E209AQ6YDHBXH2I29</depositorLei>
          <depositorStateCountry depositorState="US-NE" depositorCountry="US"/>
          <depositorUltimateParentname>MetLife, Inc.</depositorUltimateParentname>
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      </depositors>
      <uitAdmins>
        <uitAdmin>
          <uitAdminName>Brighthouse Financial, Inc.</uitAdminName>
          <uitAdminLei>5493003T8SZSHOGGAW52</uitAdminLei>
          <uitAdminStateCountry uitAdminState="US-NC" uitAdminCountry="US"/>
          <isUitAdminAffiliated>N</isUitAdminAffiliated>
          <isUitAdminSubAdmin>N</isUitAdminSubAdmin>
        </uitAdmin>
        <uitAdmin>
          <uitAdminName>Alliance-One Services, Inc.</uitAdminName>
          <uitAdminLei>74-2764079</uitAdminLei>
          <uitAdminDescOtherNumber>FEIN</uitAdminDescOtherNumber>
          <uitAdminStateCountry uitAdminState="US-DE" uitAdminCountry="US"/>
          <isUitAdminAffiliated>N</isUitAdminAffiliated>
          <isUitAdminSubAdmin>N</isUitAdminSubAdmin>
        </uitAdmin>
      </uitAdmins>
      <isUitAdminHiredTerminated>Y</isUitAdminHiredTerminated>
      <registrantSeparateInsuranceAccount isRegistrantSeparateInsuranceAccount="Y" separateAccountSeriesId="S000011671"/>
      <numOfContracts>52</numOfContracts>
      <contractSecurities>
        <contractSecurity separateAccountSecurityName="Variable Life Insurance" separateAccountContractId="C000032034" separateAccountTotalAsset="6016238.00" numContractsSold="0" grossPremiumReceived="73644.00" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="806985.00" contractValueRedeemedSection1035="0.00" numContractsAffectedRedeemed="0"/>
      </contractSecurities>
      <isRule6C7Reliance>N</isRule6C7Reliance>
      <isRule11A2Reliance>N</isRule11A2Reliance>
      <isRule12D1Dash4Reliance>N</isRule12D1Dash4Reliance>
      <isRule12D1GReliance>N</isRule12D1GReliance>
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      <isLegalProceedings>false</isLegalProceedings>
      <isProvisionFinancialSupport>false</isProvisionFinancialSupport>
      <isIPAReportInternalControl>false</isIPAReportInternalControl>
      <isInfoRequiredEO>false</isInfoRequiredEO>
      <isOtherInfoRequired>false</isOtherInfoRequired>
    </attachmentsTab>
    <signature registrantSignedName="Metropolitan Tower Separate Account One" signedDate="2024-03-07" signature="Lisa Palumbo" title="Assistant Vice President"/>
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</edgarSubmission>
