<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd">
  <headerData>
    <submissionType>NPORT-P</submissionType>
    <isConfidential>false</isConfidential>
    <filerInfo>

      <filer>
        <issuerCredentials>
          <cik>0000067160</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>


      <seriesClassInfo>
        <seriesId>S000023208</seriesId>
        <classId>C000067574</classId>
      </seriesClassInfo>


    </filerInfo>
  </headerData>
  <formData>
    <genInfo>
      <regName>MML SERIES INVESTMENT FUND</regName>
      <regFileNumber>811-02224</regFileNumber>
      <regCik>0000067160</regCik>
      <regLei>VQ1HG47DNRJG3HBJH467</regLei>
      <regStreet1>1295 State Street</regStreet1>
      <regCity>Springfield</regCity>
      <regStateConditional regCountry="US" regState="US-MA"/>
      <regZipOrPostalCode>01111-0001</regZipOrPostalCode>
      <regPhone>413-744-1000</regPhone>
      <seriesName>MML American Funds Growth Fund</seriesName>
      <seriesId>S000023208</seriesId>
      <seriesLei>549300HWJPEIS398JS44</seriesLei>
      <repPdEnd>2025-12-31</repPdEnd>
      <repPdDate>2025-06-30</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
    <fundInfo>
      <totAssets>314603416.77</totAssets>
      <totLiabs>320717.77</totLiabs>
      <netAssets>314282699.00</netAssets>
      <assetsAttrMiscSec>0.00000000</assetsAttrMiscSec>
      <assetsInvested>0.00000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.00000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.00000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.00000000</amtPayOneYrOthAffil>
      <amtPayOneYrOther>0.00000000</amtPayOneYrOther>
      <amtPayAftOneYrBanksBorr>0.00000000</amtPayAftOneYrBanksBorr>
      <amtPayAftOneYrCtrldComp>0.00000000</amtPayAftOneYrCtrldComp>
      <amtPayAftOneYrOthAffil>0.00000000</amtPayAftOneYrOthAffil>
      <amtPayAftOneYrOther>0.00000000</amtPayAftOneYrOther>
      <delayDeliv>0.00000000</delayDeliv>
      <standByCommit>0.00000000</standByCommit>
      <liquidPref>0.00000000</liquidPref>
      <cshNotRptdInCorD>0.00000000</cshNotRptdInCorD>
      <isNonCashCollateral>N</isNonCashCollateral>
      <returnInfo>
        <monthlyTotReturns>
          <monthlyTotReturn classId="C000067574" rtn1="2.04000000" rtn2="8.86000000" rtn3="6.65000000"/>
        </monthlyTotReturns>
        <othMon1 netRealizedGain="1207610.73000000" netUnrealizedAppr="4186236.09000000"/>
        <othMon2 netRealizedGain="1094822.30000000" netUnrealizedAppr="23433143.49000000"/>
        <othMon3 netRealizedGain="997053.43000000" netUnrealizedAppr="-5738459.09000000"/>
      </returnInfo>
      <mon1Flow redemption="3839280.03000000" reinvestment="0.00000000" sales="2289619.30000000"/>
      <mon2Flow redemption="3725277.52000000" reinvestment="0.00000000" sales="1760881.12000000"/>
      <mon3Flow redemption="3627121.86000000" reinvestment="0.00000000" sales="2146279.70000000"/>


    </fundInfo>
    <invstOrSecs>
      <invstOrSec>
        <name>AFIS Growth Fund</name>
        <lei>B17BZT6N2A8R48VTU480</lei>
        <title>AFIS Growth Fund</title>
        <cusip>030372403</cusip>
        <identifiers>
          <isin value="US0303724035"/>
        </identifiers>
        <balance>2437085.88000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>314603416.77000000</valUSD>
        <pctVal>100.1020475422</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2025-07-28</ncom:dateSigned>
      <ncom:nameOfApplicant>MML SERIES INVESTMENT FUND</ncom:nameOfApplicant>
      <ncom:signature>Renee Hitchcock</ncom:signature>
      <ncom:signerName>Renee Hitchcock</ncom:signerName>
      <ncom:title>CFO and Treasurer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
