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          <cik>0000036644</cik>
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      <periodOfReport>06-30-2025</periodOfReport>
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        <name>FIRST NATIONAL BANK OF OMAHA</name>
        <address>
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          <com:city>OMAHA</com:city>
          <com:stateOrCountry>NE</com:stateOrCountry>
          <com:zipCode>68197-3201</com:zipCode>
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    <signatureBlock>
      <name>Douglas Hoffman</name>
      <title>Managing Director</title>
      <phone>4026023389</phone>
      <signature>Douglas Hoffman</signature>
      <city>Omaha</city>
      <stateOrCountry>NE</stateOrCountry>
      <signatureDate>02-10-2026</signatureDate>
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