TrustCo Bank COrp ny2023 Annual meeting of Shareholders Robert J. McCormick, Chairman, President, and CEO

Agenda Remarks by the President Shareholder Questions and Comments Adjournment TrustCo Bank Corp NY Annual Meeting 2023

3 TrustCo Celebrated its 40 Year Listing Anniversary with Nasdaq on April 18, 2023 *Source: Bloomberg

Effect of 1-for-5 Reverse Stock SplitImplemented May 28, 2021 TRST Share Price leading up to reverse split: TRST Share Price steadies post-reverse split: TrustCo Bank Corp NY Annual Meeting 2023 4 Greater Volatility Greater Stability

TrustCo Bank Corp NY Total Return Before and After Reverse Split TrustCo Bank Corp NY Annual Meeting 2023 5 *Time periods shown are the 19 months leading up to, and following, the reverse stock split implemented May 28, 2021. Source: Bloomberg

TrustCo's Dividend Increases TrustCo Bank Corp NY Annual Meeting 2023 6 *Increases shown are a percent change from prior amount. Source: Blooomberg

Performance versus our Peer Group:Return on Average Assets: 77th Percentile TrustCo Bank Corp NY Annual Meeting 2023 7 TRST = 1.22 Top 77th percentile *Time period measured 1/1/2022 – 12/31/2022

Performance versus our Peer Group:Efficiency Ratio*: 78th percentile TrustCo Bank Corp NY Annual Meeting 2023 8 TRST = 50.22 Top 78th percentile *Efficiency ratio is a non-GAAP financial measure. A reconciliation of TRST’s efficiency ratio is included at the end of the presentation. *Time period measured 1/1/2022 – 12/31/2022

Performance versus our Peer Group:Tier 1 Risk Based Capital: 100th Percentile TrustCo Bank Corp NY Annual Meeting 2023 9 TRST = 18.93 100th percentile *Time period measured 1/1/2022 – 12/31/2022

Performance versus our Peer Group:Total Shareholder Return: 95th percentile TrustCo Bank Corp NY Annual Meeting 2023 10 TRST = 13.85% 95th percentile *Time period measured 1/1/2022 – 12/31/2022

TrustCo Bank Corp NY's Share Price As Compared to its Peer Group and The S&P 500 Index TrustCo Bank Corp NY Annual Meeting 2023 11

Earnings Over Time TrustCo Bank Corp NY Annual Meeting 2023 12 *$ in thousands

Loan Growth: Steady Increases Each Year TrustCo Bank Corp NY Annual Meeting 2023 13 Over a 100% increase in loan growth since 2010 *$ in thousands

Total Assets TrustCo Bank Corp NY Annual Meeting 2023 14 Total assets grew 52% from 2010 through 2022 *$ in thousands

Deposit Growth TrustCo Bank Corp NY Annual Meeting 2023 15 *$ in thousands

2022 Highlights in the Community Run For Freedom 5K Earth Day Cleanup Casselberry and Halfmoon Hurricane Ian Kentucky Flooding Regional Food Bank Capital Roots Ronald McDonald House Joseph’s House Stickball Workforce Challenge – 2nd Place for Participation TrustCo Bank Corp NY Annual Meeting 2023 16

Non-GAAP Financial Measures Reconciliation 17 TrustCo Bank Corp NY Annual Meeting 2023 Efficiency Ratio As of December 31, 2022 As of December 31, 2021 Net Interest Income (Taxable Equivalent)(Non-GAAP) $180,136 $160,409 Non-interest income (GAAP) 19,260 17,937 Less: Net gain on securities - - Less: Net gain on sale of building and net gain on sale of nonperforming loans 268 - Revenue used for efficiency ratio (Non-GAAP) $199,128 $178,346 Total Noninterest expense (GAAP) $100,309 $101,662 Less: Other real estate (income) expense, net 310 183 Expenses used for efficiency ratio (Non-GAAP) $100,009 $101,479 Efficiency Ratio 50.22% 56.90%

Thank You For Attending! TrustCo Bank Corp NY 5 Sarnowski Drive Glenville, NY 12302 TrustCo Bank Corp NY Annual Meeting 2023 18