245 Summer Street

Fidelity® Investments

Boston, MA 02210

May 31, 2022

U.S. Securities & Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549

Attention: File Room

RE: Fidelity Colchester Street Trust (the trust): File Nos. 002-74808 and 811-03320 Government Portfolio Money Market Portfolio Tax-Exempt Portfolio Treasury Only Portfolio Treasury Portfolio (the fund(s))

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Ladies and Gentlemen:

Transmitted herewith pursuant to Rule 497(j) under the Securities Act of 1933 is certification that the Prospectus(es) and Statement(s) of Additional Information with respect to the above referenced fund(s) do not differ from those filed in the most recent post-effective amendment, which was filed electronically.

Very truly yours,

/s/Cynthia Lo Bessette

Cynthia Lo Bessette

Secretary of the Trust