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        <name>Waters Corp.</name>
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        <name>Veralto Corp.</name>
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        <name>DTE Energy Co.</name>
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        <name>SPX Technologies, Inc.</name>
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        <name>Topicus.com, Inc.</name>
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        <name>PTC, Inc.</name>
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        <name>Illumina, Inc.</name>
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        <name>CAE, Inc.</name>
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        <name>Globus Medical, Inc.</name>
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        <name>Gildan Activewear, Inc.</name>
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        <name>Liberty Media Corp-Liberty Formula One</name>
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        <name>CarMax, Inc.</name>
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        <name>Revvity, Inc.</name>
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        <name>Innio NV</name>
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        <name>Liberty Media Corp-Liberty Formula One</name>
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        <name>Willis Towers Watson plc</name>
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        <name>Rentokil Initial plc</name>
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        <name>Clean Harbors, Inc.</name>
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        <name>Cooper Cos., Inc. (The)</name>
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        <name>RB Global, Inc.</name>
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        <name>API Group Corp.</name>
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        <name>Janus Henderson Cash Liquidity Fund LLC</name>
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        <name>JB Hunt Transport Services, Inc.</name>
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        <name>Arxis, Inc.</name>
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        <name>Cboe Global Markets, Inc.</name>
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        <name>N/A</name>
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        <name>Ziff Davis, Inc.</name>
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        <name>Flex Ltd.</name>
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        <name>Charles Schwab Corp. (The)</name>
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        <name>FirstService Corp.</name>
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        <name>Verisk Analytics, Inc.</name>
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        <name>Teledyne Technologies, Inc.</name>
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        <name>AppLovin Corp.</name>
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        <name>Rentokil Initial plc</name>
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        <name>Bridgebio Pharma, Inc.</name>
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        <name>LPL Financial Holdings, Inc.</name>
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        <name>ONEOK, Inc.</name>
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        <name>TransUnion</name>
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        <name>Cimpress plc</name>
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        <name>CDW Corp.</name>
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        <name>Glaukos Corp.</name>
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        <name>ON Semiconductor Corp.</name>
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        <name>NXP Semiconductors NV</name>
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        <name>Ryanair Holdings plc</name>
        <lei>635400BR2ROC1FVEBQ56</lei>
        <title>Ryanair Holdings plc</title>
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        <pctVal>0.994747428817</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>IE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Wayfair, Inc.</name>
        <lei>54930027N9ZAVYFDHK53</lei>
        <title>Wayfair, Inc., Class A</title>
        <cusip>94419L101</cusip>
        <identifiers>
          <isin value="US94419L1017"/>
        </identifiers>
        <balance>2774232.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>256394521.44000000</valUSD>
        <pctVal>1.144692710725</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Constellation Software, Inc.</name>
        <lei>549300B6PYHMCTDWQV29</lei>
        <title>Constellation Software, Inc.</title>
        <cusip>21037X100</cusip>
        <identifiers>
          <isin value="CA21037X1006"/>
        </identifiers>
        <balance>195526.00000000</balance>
        <units>NS</units>
        <currencyConditional curCd="CAD" exchangeRt="1.41810000"/>
        <valUSD>368136534.80000000</valUSD>
        <pctVal>1.643573371109</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CA</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>Forward Foreign Currency Contract</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Inhouse Asset ID" value="20775"/>
        </identifiers>
        <balance>1.00000000</balance>
        <units>NC</units>
        <curCd>N/A</curCd>
        <valUSD>-498500.52000000</valUSD>
        <pctVal>-0.00222559323</pctVal>
        <payoffProfile>N/A</payoffProfile>
        <assetCat>DFE</assetCat>
        <issuerConditional desc="Forward Foreign Currency Contract" issuerCat="OTHER"/>
        <invCountry>N/A</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <fwdDeriv derivCat="FWD">
            <counterparties>
              <counterpartyName>UBS</counterpartyName>
              <counterpartyLei>BFM8T61CT2L1QCEMIK50</counterpartyLei>
            </counterparties>
            <amtCurSold>27403267.32000000</amtCurSold>
            <curSold>USD</curSold>
            <amtCurPur>23496000.00000000</amtCurPur>
            <curPur>EUR</curPur>
            <settlementDt>2026-08-19</settlementDt>
            <unrealizedAppr>-498500.52000000</unrealizedAppr>
          </fwdDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>UL Solutions, Inc.</name>
        <lei>549300F7CUV5ESKV9P71</lei>
        <title>UL Solutions, Inc., Class A</title>
        <cusip>903731107</cusip>
        <identifiers>
          <isin value="US9037311076"/>
        </identifiers>
        <balance>1411452.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>143770500.72000000</valUSD>
        <pctVal>0.641874261849</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Burlington Stores, Inc.</name>
        <lei>5299003Z37DVGKKC1W09</lei>
        <title>Burlington Stores, Inc.</title>
        <cusip>122017106</cusip>
        <identifiers>
          <isin value="US1220171060"/>
        </identifiers>
        <balance>546782.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>173220537.60000000</valUSD>
        <pctVal>0.773356176352</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Sensata Technologies Holding plc</name>
        <lei>529900MS7KFYCY9MS037</lei>
        <title>Sensata Technologies Holding plc</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="GB00BFMBMT84"/>
        </identifiers>
        <balance>7222588.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>344806351.12000000</valUSD>
        <pctVal>1.539414003552</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Lamar Advertising Co.</name>
        <lei>529900Y8WW4MKVPTB543</lei>
        <title>Lamar Advertising Co., Class A</title>
        <cusip>512816109</cusip>
        <identifiers>
          <isin value="US5128161099"/>
        </identifiers>
        <balance>808899.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>126172066.02000000</valUSD>
        <pctVal>0.563304720627</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Canadian Pacific Kansas City Ltd.</name>
        <lei>N/A</lei>
        <title>Canadian Pacific Kansas City Ltd.</title>
        <cusip>13646K108</cusip>
        <identifiers>
          <isin value="CA13646K1084"/>
        </identifiers>
        <balance>3784687.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>327943128.55000000</valUSD>
        <pctVal>1.464126872427</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CA</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-07-30</ncom:dateSigned>
      <ncom:nameOfApplicant>Janus Investment Fund</ncom:nameOfApplicant>
      <ncom:signature>Jesper Nergaard</ncom:signature>
      <ncom:signerName>Jesper Nergaard</ncom:signerName>
      <ncom:title>VP, CFO, Treasurer, and Principal Accounting Officer</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
