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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vanguard Value ETF</name>
        <lei>N/A</lei>
        <title>Vanguard Value ETF</title>
        <cusip>922908744</cusip>
        <identifiers>
          <isin value="US9229087443"/>
        </identifiers>
        <balance>16653.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>3629188.29000000</valUSD>
        <pctVal>2.467427974099</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Enterprise Fund</name>
        <lei>TJ2Y3QQ1QRI8KQ84FR29</lei>
        <title>Janus Henderson Enterprise Fund, Class N</title>
        <cusip>47103D405</cusip>
        <identifiers>
          <isin value="US47103D4051"/>
        </identifiers>
        <balance>52767.96350000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8326256.96000000</valUSD>
        <pctVal>5.660891003989</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson High-Yield Fund</name>
        <lei>EL1Q72EQG65N1PYH1412</lei>
        <title>Janus Henderson High-Yield Fund, Class N</title>
        <cusip>47103D850</cusip>
        <identifiers>
          <isin value="US47103D8508"/>
        </identifiers>
        <balance>324646.53000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2428356.04000000</valUSD>
        <pctVal>1.651001035322</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Forty Fund</name>
        <lei>549300RDSCGTT8PWPM78</lei>
        <title>Janus Henderson Forty Fund, Class N</title>
        <cusip>47103D603</cusip>
        <identifiers>
          <isin value="US47103D6031"/>
        </identifiers>
        <balance>1540.40000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>98046.46000000</valUSD>
        <pctVal>0.066660244339</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson European Focus Fund</name>
        <lei>549300B6VJPM3NFA9J78</lei>
        <title>Janus Henderson European Focus Fund</title>
        <cusip>47103X591</cusip>
        <identifiers>
          <isin value="US47103X5914"/>
        </identifiers>
        <balance>85542.08340000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>5536283.64000000</valUSD>
        <pctVal>3.764032073928</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Inhouse Asset ID" value="ABH9984U2"/>
        </identifiers>
        <balance>1393843.24160000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1393842.42000000</valUSD>
        <pctVal>0.947651514271</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Developed World Bond Fund</name>
        <lei>JBEKRA4YPCM7Q2GORI65</lei>
        <title>Janus Henderson Developed World Bond Fund, Class N</title>
        <cusip>47103Y821</cusip>
        <identifiers>
          <isin value="US47103Y8214"/>
        </identifiers>
        <balance>2375380.30200000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>18551720.16000000</valUSD>
        <pctVal>12.61302242613</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Core MSCI Emerging Markets ETF</name>
        <lei>5493007C24Z3ZDJ0VD06</lei>
        <title>iShares Core MSCI Emerging Markets ETF</title>
        <cusip>46434G103</cusip>
        <identifiers>
          <isin value="US46434G1031"/>
        </identifiers>
        <balance>102405.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8483230.20000000</valUSD>
        <pctVal>5.767614638204</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Growth and Income Fund</name>
        <lei>5Y0L1HFYUGXP9RNAEB69</lei>
        <title>Janus Henderson Growth and Income Fund, Class N</title>
        <cusip>47103D868</cusip>
        <identifiers>
          <isin value="US47103D8680"/>
        </identifiers>
        <balance>106189.91600000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8443160.22000000</valUSD>
        <pctVal>5.740371689734</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Research Fund</name>
        <lei>TO5UP72VAT80SBB47C78</lei>
        <title>Janus Henderson Research Fund, Class N</title>
        <cusip>47103D827</cusip>
        <identifiers>
          <isin value="US47103D8276"/>
        </identifiers>
        <balance>84857.66500000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8241376.42000000</valUSD>
        <pctVal>5.603182061350</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Adaptive Risk Managed US Equity Fund</name>
        <lei>5493006DGCHLVTOMO890</lei>
        <title>Janus Henderson Adaptive Risk Managed US Equity Fund, Class N</title>
        <cusip>47103E122</cusip>
        <identifiers>
          <isin value="US47103E1221"/>
        </identifiers>
        <balance>426706.54320000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>5478912.01000000</valUSD>
        <pctVal>3.725026006050</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Emerging Markets Debt Hard Currency ETF</name>
        <lei>529900QLTSH6GU0R4M68</lei>
        <title>Janus Henderson Emerging Markets Debt Hard Currency ETF</title>
        <cusip>47103U738</cusip>
        <identifiers>
          <isin value="US47103U7384"/>
        </identifiers>
        <balance>192374.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>10350683.07000000</valUSD>
        <pctVal>7.037266440081</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Russell 2000 Value ETF</name>
        <lei>549300WJAJCAZZLTQD63</lei>
        <title>iShares Russell 2000 Value ETF</title>
        <cusip>464287630</cusip>
        <identifiers>
          <isin value="US4642876308"/>
        </identifiers>
        <balance>18814.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>4161656.80000000</valUSD>
        <pctVal>2.829444929935</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Overseas Fund</name>
        <lei>SHOQ1W1OPKO6Q4WZ8151</lei>
        <title>Janus Henderson Overseas Fund, Class N</title>
        <cusip>47103D835</cusip>
        <identifiers>
          <isin value="US47103D8359"/>
        </identifiers>
        <balance>163947.97560000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>10776300.44000000</valUSD>
        <pctVal>7.326636988282</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-07-30</ncom:dateSigned>
      <ncom:nameOfApplicant>Janus Investment Fund</ncom:nameOfApplicant>
      <ncom:signature>Jesper Nergaard</ncom:signature>
      <ncom:signerName>Jesper Nergaard</ncom:signerName>
      <ncom:title>VP, CFO, Treasurer, and Principal Accounting Officer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
