<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd "><headerData><submissionType>NPORT-P</submissionType><isConfidential>false</isConfidential><filerInfo><filer><issuerCredentials><cik>0000277751</cik><ccc>XXXXXXXX</ccc></issuerCredentials></filer><seriesClassInfo><seriesId>S000010481</seriesId><classId>C000028941</classId><classId>C000077710</classId><classId>C000077711</classId><classId>C000077712</classId><classId>C000077713</classId><classId>C000083527</classId></seriesClassInfo></filerInfo></headerData><formData><genInfo><regName>JANUS INVESTMENT FUND</regName><regFileNumber>811-01879</regFileNumber><regCik>0000277751</regCik><regLei>W6UYDZFUOFJ4HQQ32382</regLei><regStreet1>151 DETROIT STREET</regStreet1><regCity>DENVER</regCity><regStateConditional regCountry="US" regState="US-CO"/><regZipOrPostalCode>80206</regZipOrPostalCode><regPhone>3033333863</regPhone><seriesName>Janus Henderson Global Allocation Fund - Growth</seriesName><seriesId>S000010481</seriesId><seriesLei>549300099CS4YRT0YV31</seriesLei><repPdEnd>2023-06-30</repPdEnd><repPdDate>2023-06-30</repPdDate><isFinalFiling>N</isFinalFiling></genInfo><fundInfo><totAssets>226544465</totAssets><totLiabs>146503</totLiabs><netAssets>226397962</netAssets><assetsAttrMiscSec>0</assetsAttrMiscSec><assetsInvested>0</assetsInvested><amtPayOneYrBanksBorr>0</amtPayOneYrBanksBorr><amtPayOneYrCtrldComp>0</amtPayOneYrCtrldComp><amtPayOneYrOthAffil>0</amtPayOneYrOthAffil><amtPayOneYrOther>0</amtPayOneYrOther><amtPayAftOneYrBanksBorr>0</amtPayAftOneYrBanksBorr><amtPayAftOneYrCtrldComp>0</amtPayAftOneYrCtrldComp><amtPayAftOneYrOthAffil>0</amtPayAftOneYrOthAffil><amtPayAftOneYrOther>0</amtPayAftOneYrOther><delayDeliv>0</delayDeliv><standByCommit>0</standByCommit><liquidPref>0</liquidPref><cshNotRptdInCorD>0</cshNotRptdInCorD><isNonCashCollateral>N</isNonCashCollateral><returnInfo><monthlyTotReturns><monthlyTotReturn classId="C000077710" rtn1="4.11" rtn2="-2.26" rtn3="0.67"/><monthlyTotReturn classId="C000077711" rtn1="4.04" rtn2="-2.31" rtn3="0.6"/><monthlyTotReturn classId="C000083527" rtn1="4.15" rtn2="-2.24" rtn3="0.67"/><monthlyTotReturn classId="C000077712" rtn1="4.16" rtn2="-2.24" rtn3="0.67"/><monthlyTotReturn classId="C000077713" rtn1="4.15" rtn2="-2.28" rtn3="0.59"/><monthlyTotReturn classId="C000028941" rtn1="4.07" rtn2="-2.16" rtn3="0.67"/></monthlyTotReturns><monthlyReturnCats><commodityContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></commodityContracts><creditContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></creditContracts><equityContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></equityContracts><foreignExchgContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="0" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></foreignExchgContracts><interestRtContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></interestRtContracts><otherContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></otherContracts></monthlyReturnCats><othMon1 netRealizedGain="-80355" netUnrealizedAppr="8570466"/><othMon2 netRealizedGain="-446858" netUnrealizedAppr="-4232250"/><othMon3 netRealizedGain="-133669" netUnrealizedAppr="1572701"/></returnInfo><mon1Flow redemption="-1481737.06" reinvestment="0" sales="768457.37"/><mon2Flow redemption="-2440409.57" reinvestment="0" sales="811673.53"/><mon3Flow redemption="-1983286.52" reinvestment="0" sales="1085315.74"/><varInfo><fundsDesignatedInfo><nameDesignatedIndex>Global Growth Allocation Index (80% ACWI / 20% GlobalAgg)</nameDesignatedIndex><indexIdentifier>IX80ACWI20GLBLAGG</indexIdentifier></fundsDesignatedInfo></varInfo></fundInfo><invstOrSecs><invstOrSec><name>Janus Henderson Forty Fund</name><lei>549300RDSCGTT8PWPM78</lei><title>Janus Henderson Forty Fund - Class N Shares</title><cusip>47103D603</cusip><identifiers><isin value="US47103D6031"/><ticker value="JFRNX US"/><other otherDesc="B8G4506" value="Sedol"/></identifiers><balance>196995</balance><units>NS</units><curCd>USD</curCd><valUSD>9485332</valUSD><pctVal>4.19</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson US Managed Volatility Fund</name><lei>5493006DGCHLVTOMO890</lei><title>Janus Henderson U.S. Managed Volatility Fund - Class N Shares</title><cusip>47103E122</cusip><identifiers><isin value="US47103E1221"/><ticker value="JRSNX US"/></identifiers><balance>1809914</balance><units>NS</units><curCd>USD</curCd><valUSD>17773355</valUSD><pctVal>7.85</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Real Estate Fund</name><lei>A5263I2XDOP1IOXZPS12</lei><title>Janus Henderson Global Real Estate Fund - Class N Shares</title><cusip>47103Y623</cusip><identifiers><isin value="US47103Y6234"/><ticker value="JERNX US"/><other otherDesc="BG11809" value="Sedol"/></identifiers><balance>611635</balance><units>NS</units><curCd>USD</curCd><valUSD>6868658</valUSD><pctVal>3.034</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Investment Fund</name><lei>W6UYDZFUOFJ4HQQ32382</lei><title>Janus Henderson U.S. Dividend Income Fund - Class N Shares</title><cusip>47103Y482</cusip><identifiers><isin value="US47103Y4825"/><ticker value="JNDVX"/><other otherDesc="BNBVL76" value="Sedol"/></identifiers><balance>847824</balance><units>NS</units><curCd>USD</curCd><valUSD>8673236</valUSD><pctVal>3.831</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Emerging Markets Fund</name><lei>549300OPPFBZE65UIL89</lei><title>Janus Henderson Emerging Markets Fund - Class N Shares</title><cusip>47103X716</cusip><identifiers><isin value="US47103X7167"/><ticker value="HEMRX US"/><other otherDesc="BF334V9" value="Sedol"/></identifiers><balance>2095603</balance><units>NS</units><curCd>USD</curCd><valUSD>18567046</valUSD><pctVal>8.201</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Growth and Inc</name><lei>5Y0L1HFYUGXP9RNAEB69</lei><title>Janus Henderson Growth and Income Fund - Class N Shares</title><cusip>47103D868</cusip><identifiers><isin value="US47103D8680"/><ticker value="JDNNX US"/><other otherDesc="BHLSPJ5" value="Sedol"/></identifiers><balance>132311</balance><units>NS</units><curCd>USD</curCd><valUSD>9275016</valUSD><pctVal>4.097</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Research Fund</name><lei>C0GC60LX4ZGTZPLZA692</lei><title>Janus Henderson Global Research Fund - Class N Shares</title><cusip>47103D777</cusip><identifiers><isin value="US47103D7773"/><ticker value="JDWNX US"/><other otherDesc="BHLSPF1" value="Sedol"/></identifiers><balance>68412</balance><units>NS</units><curCd>USD</curCd><valUSD>6291127</valUSD><pctVal>2.779</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Adaptive Global Allocation Fund</name><lei>549300HN0SRQIOHVMK11</lei><title>Janus Henderson Adaptive Global Allocation Fund - Class N Shares</title><cusip>47103D231</cusip><identifiers><isin value="US47103D2311"/><ticker value="JAGNX US"/><other otherDesc="BYRY701" value="Sedol"/></identifiers><balance>1246310</balance><units>NS</units><curCd>USD</curCd><valUSD>12450641</valUSD><pctVal>5.499</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson European Focus</name><lei>549300B6VJPM3NFA9J78</lei><title>Janus Henderson European Focus Fund - Class N Shares</title><cusip>47103X591</cusip><identifiers><isin value="US47103X5914"/><ticker value="HFERX US"/><other otherDesc="BF5LNY7" value="Sedol"/></identifiers><balance>116526</balance><units>NS</units><curCd>USD</curCd><valUSD>4878938</valUSD><pctVal>2.155</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Flexible Bond Fund</name><lei>C8DSHUK27T5OOEDPW945</lei><title>Janus Henderson Flexible Bond Fund - Class N Shares</title><cusip>47103D504</cusip><identifiers><isin value="US47103D5041"/><ticker value="JDFNX US"/><other otherDesc="B796JF5" value="Sedol"/></identifiers><balance>1359791</balance><units>NS</units><curCd>USD</curCd><valUSD>12632457</valUSD><pctVal>5.58</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Enterprise Fund</name><lei>TJ2Y3QQ1QRI8KQ84FR29</lei><title>Janus Henderson Enterprise Fund - Class N Shares</title><cusip>47103D405</cusip><identifiers><isin value="US47103D4051"/><ticker value="JDMNX US"/><other otherDesc="B8F0PT4" value="Sedol"/></identifiers><balance>66500</balance><units>NS</units><curCd>USD</curCd><valUSD>9123842</valUSD><pctVal>4.03</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Short-Term Bond Fund</name><lei>0IWXBJ4S82V64ZFH4582</lei><title>Janus Henderson Short-Term Bond Fund - Class N Shares</title><cusip>47103D819</cusip><identifiers><isin value="US47103D8193"/><ticker value="JSHNX US"/><other otherDesc="B7FTRS7" value="Sedol"/></identifiers><balance>1266207</balance><units>NS</units><curCd>USD</curCd><valUSD>3532719</valUSD><pctVal>1.56</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Equity Income Fund</name><lei>549300LMDPEFBD67P863</lei><title>Janus Henderson Global Equity Income Fund - Class N Shares</title><cusip>47103X484</cusip><identifiers><isin value="US47103X4842"/><ticker value="HFQRX US"/><other otherDesc="BF336R9" value="Sedol"/></identifiers><balance>1430856</balance><units>NS</units><curCd>USD</curCd><valUSD>8628063</valUSD><pctVal>3.811</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Overseas Fund</name><lei>SHOQ1W1OPKO6Q4WZ8151</lei><title>Janus Henderson Overseas Fund - Class N Shares</title><cusip>47103D835</cusip><identifiers><isin value="US47103D8359"/><ticker value="JDINX US"/><other otherDesc="B8G4681" value="Sedol"/></identifiers><balance>457875</balance><units>NS</units><curCd>USD</curCd><valUSD>19500908</valUSD><pctVal>8.614</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson High-Yield Fun</name><lei>EL1Q72EQG65N1PYH1412</lei><title>Janus Henderson High-Yield Fund - Class N Shares</title><cusip>47103D850</cusip><identifiers><isin value="PLPEKAO00016"/><ticker value="JHYNX US"/><other otherDesc="5473113" value="Sedol"/></identifiers><balance>114735</balance><units>NS</units><curCd>USD</curCd><valUSD>808883</valUSD><pctVal>0.357</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Contrarian Fund</name><lei>05B6OC1W7N8NJQRIYS63</lei><title>Janus Henderson Contrarian Fund - Class N Shares</title><cusip>47103D207</cusip><identifiers><isin value="US47103D2071"/><ticker value="JCNNX US"/><other otherDesc="BHLSPK6" value="Sedol"/></identifiers><balance>450924</balance><units>NS</units><curCd>USD</curCd><valUSD>11940475</valUSD><pctVal>5.274</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Select Fund</name><lei>YTNSNBKH822ME5K53798</lei><title>Janus Henderson Global Select Fund - Class N Shares</title><cusip>47103D876</cusip><identifiers><isin value="US47103D8763"/><ticker value="JSLNX US"/><other otherDesc="BHLSPG2" value="Sedol"/></identifiers><balance>599610</balance><units>NS</units><curCd>USD</curCd><valUSD>10175383</valUSD><pctVal>4.494</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Triton Fund</name><lei>GKBLW4JP2S45CI5N6B55</lei><title>Janus Henderson Triton Fund - Class N Shares</title><cusip>47103D793</cusip><identifiers><isin value="US47103D7930"/><ticker value="JGMNX US"/><other otherDesc="B82VS68" value="Sedol"/></identifiers><balance>273722</balance><units>NS</units><curCd>USD</curCd><valUSD>7519152</valUSD><pctVal>3.321</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Bond Fund</name><lei>549300JVFLA561A67H59</lei><title>Janus Henderson Global Bond Fund - Class N Shares</title><cusip>47103D801</cusip><identifiers><isin value="US47103D8011"/><ticker value="JGLNX US"/><other otherDesc="BHLSPN9" value="Sedol"/></identifiers><balance>3577735</balance><units>NS</units><curCd>USD</curCd><valUSD>27620111</valUSD><pctVal>12.2</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Asia Equity Fund</name><lei>549300WZU4CKDFTFYJ24</lei><title>Janus Henderson Asia Equity Fund - Class N Shares</title><cusip>47103Y649</cusip><identifiers><isin value="US47103Y6499"/><ticker value="JAQNX US"/><other otherDesc="BG11821" value="Sedol"/></identifiers><balance>668608</balance><units>NS</units><curCd>USD</curCd><valUSD>6579106</valUSD><pctVal>2.906</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Small-Mid Cap</name><lei>549300HQ5RZ4HJ4T1U70</lei><title>Janus Henderson Small-Mid Cap Value Fund - Class N Shares</title><cusip>47103D736</cusip><identifiers><isin value="INE242A01010"/><ticker value="JVSNX US"/><other otherDesc="6253767" value="Sedol"/></identifiers><balance>831281</balance><units>NS</units><curCd>USD</curCd><valUSD>11263861</valUSD><pctVal>4.975</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Multi-Sector Income Fund Class N Shares</name><lei>549300WARVMK66TM8315</lei><title>Janus Henderson Multi-Sector Income Fund Class N Shares</title><cusip>47103D496</cusip><identifiers><isin value="US47103D4960"/><ticker value="JMTNX US"/><other otherDesc="BK8G0L0" value="Sedol"/></identifiers><balance>47248</balance><units>NS</units><curCd>USD</curCd><valUSD>398770</valUSD><pctVal>0.176</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson AAA CLO ETF</name><lei>549300N7UZ9PO4OVKA94</lei><title>Janus Henderson AAA CLO</title><cusip>47103U845</cusip><identifiers><isin value="US47103U8457"/><ticker value="JAAA US"/><other otherDesc="BKVH8V6" value="Sedol"/></identifiers><balance>37612</balance><units>NS</units><curCd>USD</curCd><valUSD>1874879</valUSD><pctVal>0.828</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson US Holdings Inc</name><lei>8T2ZO87FU1KVPZIG9I59</lei><title>Janus Henderson Cash Liquidity Fund</title><cusip>N/A</cusip><identifiers><isin value="DU0000100962"/></identifiers><balance>153999</balance><units>NS</units><curCd>USD</curCd><valUSD>154030</valUSD><pctVal>0.068</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>PF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>2</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec></invstOrSecs><explntrNotes><explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/></explntrNotes><signature><ncom:dateSigned>2023-08-14</ncom:dateSigned><ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant><ncom:signature>JESPER NERGAARD</ncom:signature><ncom:signerName>JESPER NERGAARD</ncom:signerName><ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title></signature></formData></edgarSubmission>
