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value="Sedol"/></identifiers><balance>249798</balance><units>NS</units><curCd>USD</curCd><valUSD>706928</valUSD><pctVal>0.315</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Equity Income Fund</name><lei>549300LMDPEFBD67P863</lei><title>Janus Henderson Global Equity Income Fund - Class N Shares</title><cusip>47103X484</cusip><identifiers><isin value="US47103X4842"/><ticker value="HFQRX US"/><other otherDesc="BF336R9" value="Sedol"/></identifiers><balance>1890693</balance><units>NS</units><curCd>USD</curCd><valUSD>11608853</valUSD><pctVal>5.178</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Overseas Fund</name><lei>SHOQ1W1OPKO6Q4WZ8151</lei><title>Janus Henderson Overseas Fund - Class N Shares</title><cusip>47103D835</cusip><identifiers><isin value="US47103D8359"/><ticker value="JDINX US"/><other otherDesc="B8G4681" value="Sedol"/></identifiers><balance>554298</balance><units>NS</units><curCd>USD</curCd><valUSD>23252783</valUSD><pctVal>10.373</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson High-Yield Fun</name><lei>EL1Q72EQG65N1PYH1412</lei><title>Janus Henderson High-Yield Fund - Class N Shares</title><cusip>47103D850</cusip><identifiers><isin value="PLPEKAO00016"/><ticker value="JHYNX US"/><other otherDesc="5473113" value="Sedol"/></identifiers><balance>397297</balance><units>NS</units><curCd>USD</curCd><valUSD>2800947</valUSD><pctVal>1.249</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Contrarian Fund</name><lei>05B6OC1W7N8NJQRIYS63</lei><title>Janus Henderson Contrarian Fund - Class N Shares</title><cusip>47103D207</cusip><identifiers><isin value="US47103D2071"/><ticker value="JCNNX US"/><other otherDesc="BHLSPK6" value="Sedol"/></identifiers><balance>533824</balance><units>NS</units><curCd>USD</curCd><valUSD>13388308</valUSD><pctVal>5.972</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Select Fund</name><lei>YTNSNBKH822ME5K53798</lei><title>Janus Henderson Global Select Fund - Class N Shares</title><cusip>47103D876</cusip><identifiers><isin value="US47103D8763"/><ticker value="JSLNX US"/><other otherDesc="BHLSPG2" value="Sedol"/></identifiers><balance>809950</balance><units>NS</units><curCd>USD</curCd><valUSD>13404680</valUSD><pctVal>5.98</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Triton Fund</name><lei>GKBLW4JP2S45CI5N6B55</lei><title>Janus Henderson Triton Fund - Class N Shares</title><cusip>47103D793</cusip><identifiers><isin value="US47103D7930"/><ticker value="JGMNX US"/><other otherDesc="B82VS68" value="Sedol"/></identifiers><balance>321631</balance><units>NS</units><curCd>USD</curCd><valUSD>8539307</valUSD><pctVal>3.809</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Bond Fund</name><lei>549300JVFLA561A67H59</lei><title>Janus Henderson Global Bond Fund - Class N Shares</title><cusip>47103D801</cusip><identifiers><isin value="US47103D8011"/><ticker value="JGLNX US"/><other otherDesc="BHLSPN9" value="Sedol"/></identifiers><balance>2050054</balance><units>NS</units><curCd>USD</curCd><valUSD>16318431</valUSD><pctVal>7.279</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Asia Equity Fund</name><lei>549300WZU4CKDFTFYJ24</lei><title>Janus Henderson Asia Equity Fund - Class N Shares</title><cusip>47103Y649</cusip><identifiers><isin value="US47103Y6499"/><ticker value="JAQNX US"/><other otherDesc="BG11821" value="Sedol"/></identifiers><balance>648656</balance><units>NS</units><curCd>USD</curCd><valUSD>6512505</valUSD><pctVal>2.905</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Small-Mid Cap</name><lei>549300HQ5RZ4HJ4T1U70</lei><title>Janus Henderson Small-Mid Cap Value Fund - Class N Shares</title><cusip>47103D736</cusip><identifiers><isin value="INE242A01010"/><ticker value="JVSNX US"/><other otherDesc="6253767" value="Sedol"/></identifiers><balance>991417</balance><units>NS</units><curCd>USD</curCd><valUSD>12838849</valUSD><pctVal>5.727</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Multi-Sector Income Fund Class N Shares</name><lei>549300WARVMK66TM8315</lei><title>Janus Henderson Multi-Sector Income Fund Class N Shares</title><cusip>47103D496</cusip><identifiers><isin value="US47103D4960"/><ticker value="JMTNX US"/><other otherDesc="BK8G0L0" value="Sedol"/></identifiers><balance>98669</balance><units>NS</units><curCd>USD</curCd><valUSD>836712</valUSD><pctVal>0.373</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson AAA CLO ETF</name><lei>549300N7UZ9PO4OVKA94</lei><title>Janus Henderson AAA CLO</title><cusip>47103U845</cusip><identifiers><isin value="US47103U8457"/><ticker value="JAAA US"/><other otherDesc="BKVH8V6" value="Sedol"/></identifiers><balance>53546</balance><units>NS</units><curCd>USD</curCd><valUSD>2651062</valUSD><pctVal>1.183</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Capital Group Inc</name><lei>8T2ZO87FU1KVPZIG9I59</lei><title>Janus Henderson Cash Liquidity Fund</title><cusip>N/A</cusip><identifiers><isin value="DU0000100962"/></identifiers><balance>118214</balance><units>NS</units><curCd>USD</curCd><valUSD>118237</valUSD><pctVal>0.053</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>PF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>2</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec></invstOrSecs><explntrNotes><explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/></explntrNotes><signature><ncom:dateSigned>2023-05-15</ncom:dateSigned><ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant><ncom:signature>JESPER NERGAARD</ncom:signature><ncom:signerName>JESPER NERGAARD</ncom:signerName><ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title></signature></formData><documents>XXXX</documents></edgarSubmission>
