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value="Sedol"/></identifiers><balance>4441763</balance><units>NS</units><curCd>USD</curCd><valUSD>220933292</valUSD><pctVal>1.12</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>LVMH Moet Hennessy Louis Vuitt</name><lei>IOG4E947OATN0KJYSD45</lei><title>LVMH Moet Hennessy Louis Vuitton SE</title><cusip>F58485115</cusip><identifiers><isin value="FR0000121014"/><ticker value="MC FP"/><other otherDesc="4061412" value="Sedol"/></identifiers><balance>466506</balance><units>NS</units><currencyConditional curCd="EUR" 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value="Sedol"/></identifiers><balance>2819799</balance><units>NS</units><curCd>USD</curCd><valUSD>1007739767</valUSD><pctVal>5.11</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Match Group Inc</name><lei>15OMOPB0A8K0EFUVD031</lei><title>Match Group Inc</title><cusip>57667L107</cusip><identifiers><isin value="US57667L1070"/><ticker value="MTCH US"/><other otherDesc="BK80XH9" value="Sedol"/></identifiers><balance>3109763</balance><units>NS</units><curCd>USD</curCd><valUSD>338155629</valUSD><pctVal>1.715</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Microsoft Corp</name><lei>INR2EJN1ERAN0W5ZP974</lei><title>MICROSOFT CORP</title><cusip>594918104</cusip><identifiers><isin value="US5949181045"/><ticker value="MSFT US"/><other otherDesc="2588173" value="Sedol"/></identifiers><balance>6432481</balance><units>NS</units><curCd>USD</curCd><valUSD>1983198217</valUSD><pctVal>10.057</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Netflix Inc</name><lei>549300Y7VHGU0I7CE873</lei><title>Netflix Inc</title><cusip>64110L106</cusip><identifiers><isin value="US64110L1061"/><ticker value="NFLX US"/><other otherDesc="2857817" value="Sedol"/></identifiers><balance>862215</balance><units>NS</units><curCd>USD</curCd><valUSD>322977117</valUSD><pctVal>1.638</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>NIKE Inc</name><lei>787RXPR0UX0O0XUXPZ81</lei><title>NIKE Inc - Class B</title><cusip>654106103</cusip><identifiers><isin value="US6541061031"/><ticker value="NKE US"/><other otherDesc="2640147" value="Sedol"/></identifiers><balance>1866804</balance><units>NS</units><curCd>USD</curCd><valUSD>251197146</valUSD><pctVal>1.274</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>NVIDIA Corp</name><lei>549300S4KLFTLO7GSQ80</lei><title>NVIDIA Corp</title><cusip>67066G104</cusip><identifiers><isin value="US67066G1040"/><ticker value="NVDA US"/><other otherDesc="2379504" value="Sedol"/></identifiers><balance>941728</balance><units>NS</units><curCd>USD</curCd><valUSD>256959902</valUSD><pctVal>1.303</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Procter &amp; Gamble Co/The</name><lei>2572IBTT8CCZW6AU4141</lei><title>Procter &amp; Gamble Co</title><cusip>742718109</cusip><identifiers><isin value="US7427181091"/><ticker value="PG US"/><other otherDesc="2704407" value="Sedol"/></identifiers><balance>2402120</balance><units>NS</units><curCd>USD</curCd><valUSD>367043936</valUSD><pctVal>1.861</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Rivian Automotive Inc</name><lei>N/A</lei><title>Rivian Automotive Inc - Class A</title><cusip>76954A103</cusip><identifiers><isin value="US76954A1034"/><ticker value="RIVN US"/><other otherDesc="BL98841" value="Sedol"/></identifiers><balance>2075481</balance><units>NS</units><curCd>USD</curCd><valUSD>104272165</valUSD><pctVal>0.529</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><loanByFundCondition isLoanByFund="Y" loanVal="80959286.1"/></securityLending></invstOrSec><invstOrSec><name>Sherwin-Williams Co/The</name><lei>Z15BMIOX8DDH0X2OBP21</lei><title>Sherwin-Williams Co</title><cusip>824348106</cusip><identifiers><isin value="US8243481061"/><ticker value="SHW US"/><other otherDesc="2804211" value="Sedol"/></identifiers><balance>1134616</balance><units>NS</units><curCd>USD</curCd><valUSD>283222846</valUSD><pctVal>1.436</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Snap Inc</name><lei>549300JNC0L6VP8A2Q35</lei><title>Snap Inc - Class A</title><cusip>83304A106</cusip><identifiers><isin value="US83304A1060"/><ticker value="SNAP US"/><other otherDesc="BD8DJ71" value="Sedol"/></identifiers><balance>16845728</balance><units>NS</units><curCd>USD</curCd><valUSD>606277751</valUSD><pctVal>3.074</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Block Inc</name><lei>549300OHIIUWSTIZME52</lei><title>Square Inc</title><cusip>852234103</cusip><identifiers><isin value="US8522341036"/><ticker value="SQ US"/><other otherDesc="BYNZGK1" 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value="DU0000100962"/></identifiers><balance>346666424</balance><units>NS</units><curCd>USD</curCd><valUSD>346701091</valUSD><pctVal>1.758</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>PF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>2</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Cash Collateral Fund</name><lei>N/A</lei><title>Janus Henderson Cash Collateral Fund</title><cusip>N/A</cusip><identifiers><other otherDesc="896" value="Internal ID"/></identifiers><balance>66276288</balance><units>NS</units><curCd>USD</curCd><valUSD>66276288</valUSD><pctVal>0.336</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>PF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>2</fairValLevel><securityLending><cashCollateralCondition cashCollateralVal="66276288" isCashCollateral="Y"/><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Twilio Inc</name><lei>5493004W8TRGD63APX93</lei><title>Twilio Inc</title><cusip>90138F102</cusip><identifiers><isin value="US90138F1021"/><ticker value="TWLO US"/><other otherDesc="BD6P5Q0" value="Sedol"/></identifiers><balance>1966590</balance><units>NS</units><curCd>USD</curCd><valUSD>324113698</valUSD><pctVal>1.644</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>UnitedHealth Group Inc</name><lei>549300GHBMY8T5GXDE41</lei><title>UnitedHealth Group Inc</title><cusip>91324P102</cusip><identifiers><isin value="US91324P1021"/><ticker value="UNH US"/><other otherDesc="2917766" value="Sedol"/></identifiers><balance>1022505</balance><units>NS</units><curCd>USD</curCd><valUSD>521446875</valUSD><pctVal>2.644</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Unity Software Inc</name><lei>549300D1ESJMGNQNG432</lei><title>Unity Software Inc</title><cusip>91332U101</cusip><identifiers><isin value="US91332U1016"/><ticker value="U US"/><other otherDesc="BLFDXH8" value="Sedol"/></identifiers><balance>1398232</balance><units>NS</units><curCd>USD</curCd><valUSD>138718597</valUSD><pctVal>0.703</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Workday Inc</name><lei>549300J0DYC0N31V7G13</lei><title>Workday Inc</title><cusip>98138H101</cusip><identifiers><isin value="US98138H1014"/><ticker value="WDAY US"/><other otherDesc="B8K6ZD1" value="Sedol"/></identifiers><balance>2119582</balance><units>NS</units><curCd>USD</curCd><valUSD>507555106</valUSD><pctVal>2.574</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Atlassian Corp PLC</name><lei>549300AGBII4MWBVJY22</lei><title>Atlassian Corp PLC - Class A</title><cusip>G06242104</cusip><identifiers><isin value="GB00BZ09BD16"/><ticker value="TEAM US"/><other otherDesc="BZ09BD1" value="Sedol"/></identifiers><balance>557400</balance><units>NS</units><curCd>USD</curCd><valUSD>163780842</valUSD><pctVal>0.831</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>ASML Holding NV</name><lei>724500Y6DUVHQD6OXN27</lei><title>ASML Holding NV</title><cusip>N07059210</cusip><identifiers><isin value="USN070592100"/><ticker value="ASML US"/><other otherDesc="B908F01" value="Sedol"/></identifiers><balance>909866</balance><units>NS</units><curCd>USD</curCd><valUSD>607726797</valUSD><pctVal>3.082</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>TSMC</name><lei>549300KB6NK5SBD14S87</lei><title>Taiwan Semiconductor Manufacturing Co Ltd (ADR)</title><cusip>874039100</cusip><identifiers><isin value="US8740391003"/><ticker value="TSM US"/><other otherDesc="2113382" value="Sedol"/></identifiers><balance>2818336</balance><units>NS</units><curCd>USD</curCd><valUSD>293839711</valUSD><pctVal>1.49</pctVal><payoffProfile>Long</payoffProfile><assetConditional assetCat="OTHER" desc="GDR"/><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>N/A</name><lei>N/A</lei><title>Grab Holdings Ltd</title><cusip>N/A</cusip><identifiers><isin value="KYG4124C1179"/><ticker value="GRABW US"/><other otherDesc="G4124C117" value="Internal ID"/></identifiers><balance>860268</balance><units>NS</units><curCd>USD</curCd><valUSD>662406</valUSD><pctVal>0.003</pctVal><payoffProfile>N/A</payoffProfile><assetCat>DE</assetCat><issuerCat>CORP</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><derivativeInfo><optionSwaptionWarrantDeriv derivCat="WAR"><counterparties><counterpartyName>N/A</counterpartyName><counterpartyLei>N/A</counterpartyLei></counterparties><putOrCall>Call</putOrCall><writtenOrPur>Purchased</writtenOrPur><descRefInstrmnt><otherRefInst><issuerName>Grab Holdings Ltd</issuerName><issueTitle>Grab Holdings Ltd</issueTitle><identifiers><cusip value="G4124C109"/><isin value="KYG4124C1096"/><ticker value="GRAB US"/></identifiers></otherRefInst></descRefInstrmnt><shareNo>860268</shareNo><exercisePrice>11.5</exercisePrice><exercisePriceCurCd>USD</exercisePriceCurCd><expDt>2026-12-01</expDt><delta>XXXX</delta><unrealizedAppr>-2087302</unrealizedAppr></optionSwaptionWarrantDeriv></derivativeInfo><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec></invstOrSecs><explntrNotes><explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/></explntrNotes><signature><ncom:dateSigned>2022-05-15</ncom:dateSigned><ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant><ncom:signature>JESPER NERGAARD</ncom:signature><ncom:signerName>JESPER NERGAARD</ncom:signerName><ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title></signature></formData></edgarSubmission>
