NPORT-Pfalse0000277751XXXXXXXXS000010488C000028950C000083533C000100475C000100476C000100477C000100478C000114881JANUS INVESTMENT FUND811-018790000277751W6UYDZFUOFJ4HQQ32382151 DETROIT STREETDENVER802063033333863Janus Henderson Venture FundS000010488M8SXC17QOUZM03NWSY642020-09-302020-09-30N3422567562439736643378593898000000000020361850000NN/AN/ACooperatieve Rabobank U.A., New York BranchN/A8507790PAUSD85077900.252LongSTIVCORPUSN22020-10-01Fixed0.08NNNNNN/AN/ARoyal Bank of CanadaN/A17003045PAUSD170030450.503LongSTIVCORPUSN22020-10-01Fixed0.07NNNNNAcceleron Pharma Inc529900TQRID2UY164805Acceleron Pharma Inc00434H108131824NSUSD148341550.439LongECCORPUSN1NNNAkero Therapeutics Inc549300SI3KG74LBE2955Akero Therapeutics Inc00973Y108440857NSUSD135739870.402LongECCORPUSN1NNNAlamo Group Inc54930080C93RVZRSDV26Alamo Group Inc011311107156863NSUSD169459100.502LongECCORPUSN1NNNAltair Engineering Inc549300FJ89HZ6GU9S994Altair Engineering Inc021369103628531NSUSD263857310.781LongECCORPUSN1NNNAMERCO5493003WIDMLDHYC6Y24AMERCO02358610053454NSUSD190285550.563LongECCORPUSN1NNNAmicus Therapeutics Inc549300MRD1SMUTNBPQ53Amicus Therapeutics Inc03152W1091359188NSUSD191917350.568LongECCORPUSN1NNNArcturus Therapeutics HoldingsN/AArcturus Therapeutics Holdings Inc03969T109131208NSUSD56288230.167LongECCORPUSN1NNNAssetmark Financial Holdings I5493001K4EDHT2T6W812Assetmark Financial Holdings Inc04546L106593381NSUSD129001030.382LongECCORPUSN1NNNAxogen IncN/AAXOGEN INC05463X1061176910NSUSD136874630.405LongECCORPUSN1NNNBelden Inc68BKYO1JC791TTJHUE82Belden Inc077454106318185NSUSD99019170.293LongECCORPUSN1NNNBellRing Brands IncN/ABellRing Brands Inc079823100707179NSUSD146668920.434LongECCORPUSN1NNNBio-Techne Corp54930073RLKQ51TILZ35Bio-Techne Corp09073M104146355NSUSD362565241.073LongECCORPUSN1NNNBlackbaud Inc54930056X3HKFMNG3754Blackbaud Inc09227Q100675263NSUSD376999331.116LongECCORPUSN1NNNBrady Corp549300G2LEQOFQC8GY34Brady Corp104674106747368NSUSD299096670.885LongECCORPUSN1NNNBridgebio Pharma Inc54930028YG89VTY2J114Bridgebio Pharma Inc10806X102502576NSUSD188566520.558LongECCORPUSN1NNBroadridge Financial SolutionsN/ABroadridge Financial Solutions Inc11133T103404541NSUSD533994121.581LongECCORPUSN1NNNCSW Industrials Inc549300SYOEO3RVQ7FB83CSW Industrials Inc126402106539738NSUSD416947611.234LongECCORPUSN1NNNCarter's Inc5299003WAQK4R3L3ZU36Carter's Inc146229109189127NSUSD163746160.485LongECCORPUSN1NNNCasey's General Stores Inc549300CAFWSI86V66Q65Casey's General Stores Inc147528103119966NSUSD213119600.631LongECCORPUSN1NNNCatalent IncN/ACatalent Inc1488061021315929NSUSD1127224783.336LongECCORPUSN1NNNChannelAdvisor Corp549300KE5CWXBSGWXT83ChannelAdvisor Corp1591791001800218NSUSD260491540.771LongECCORPUSN1NNNChase CorpN/AChase Corp16150R104129009NSUSD123074590.364LongECCORPUSN1NNNCodexis IncN/ACodexis Inc1920051061188999NSUSD139588480.413LongECCORPUSN1NNNConstruction Partners Inc549300G4ICXJKQN32X70Construction Partners Inc21044C107917598NSUSD167002840.494LongECCORPUSN1NNNDescartes Systems Group Inc/Th875500NEPA7JI5EOMN45Descartes Systems Group Inc2499061081054700NS600863711.778LongECCORPCAN1NNNCryoPort IncN/ACryoPort Inc229050307862955NSUSD409040671.211LongECCORPUSN1NNDeciphera Pharmaceuticals Inc529900QR4WAPYY0CBF25Deciphera Pharmaceuticals Inc24344T101304303NSUSD156107440.462LongECCORPUSN1NNNDunkin' Brands Group Inc549300311IRV47UCTO62Dunkin' Brands Group Inc265504100275350NSUSD225539190.668LongECCORPUSN1NNNEagle Pharmaceuticals Inc/DE549300TN20OLYTJVP152Eagle Pharmaceuticals Inc/DE269796108465665NSUSD197814490.585LongECCORPUSN1NNNEasterly Government Properties549300ERMCEFC6S8HL77Easterly Government Properties Inc27616P103993442NSUSD222630350.659LongECCORPUSN1NNNEnerSys7ZS3DE5PN30BYUJFWP05EnerSys29275Y102372503NSUSD250024010.74LongECCORPUSN1NNNEnvestnet Inc549300HLWZ5OKIDM8737Envestnet Inc29404K106493539NSUSD380814691.127LongECCORPUSN1NNNEuronet Worldwide IncYG3575C4DSHJPA5KET68Euronet Worldwide Inc298736109418209NSUSD380988401.128LongECCORPUSN1NNNEverbridge Inc549300WCY0UMR64ATW81Everbridge Inc29978A104197880NSUSD248794520.736LongECCORPUSN1NNNFibroGen Inc549300Q914ULWWY95822FibroGen Inc31572Q808628104NSUSD258276360.764LongECCORPUSN1NNNFirstService Corp5493000XUDIV75BCF118FirstService Corp33767E202240928NSUSD317759940.941LongECCORPCAN1NNNfrontdoor Inc5493008RB0JZJI3QGZ06frontdoor Inc35905A109680032NSUSD264600450.783LongECCORPUSN1NNNHB Fuller CoJSMX3Z5FOYM8V0Q52357HB Fuller Co359694106389529NSUSD178326380.528LongECCORPUSN1NNNGlaukos Corp5299004JA1LHE21M6T14Glaukos Corp377322102308848NSUSD152941530.453LongECCORPUSN1NNGlobal Blood Therapeutics Inc549300U4G0QKK99GRT15Global Blood Therapeutics Inc37890U108289095NSUSD159406980.472LongECCORPUSN1NNNGlobus Medical Inc5299003M2P4CIK0RF205Globus Medical Inc379577208664587NSUSD329103480.974LongECCORPUSN1NNNIntelligent Systems CorpN/AIntelligent Systems Corp PPN/A95103NSUSD35217590.104LongECCORPUSN2NNNVaxcyte IncN/AVaxcyte IncN/A604217NSUSD283444240.839LongECCORPUSN2NNNGuidewire Software Inc549300WXSG0J8IXZCK32Guidewire Software Inc40171V100191850NSUSD200042000.592LongECCORPUSN1NNNHain Celestial Group Inc/The5493000S8QXWHLIXDP75Hain Celestial Group Inc405217100639577NSUSD219374910.649LongECCORPUSN1NNNHalozyme Therapeutics Inc529900242I3SV9AGM753Halozyme Therapeutics Inc40637H109805567NSUSD211703010.627LongECCORPUSN1NNNHealthEquity Inc529900LRJU1RAFQHM467HealthEquity Inc42226A107299965NSUSD154092020.456LongECCORPUSN1NNNHEICO Corp529900O1DTDLCJ7L0I14HEICO Corp422806208150267NSUSD133226720.394LongECCORPUSN1NNNHeron Therapeutics Inc549300DR2I1DOPD2IX53Heron Therapeutics Inc427746102583094NSUSD86414530.256LongECCORPUSN1NNNHeska Corp529900O1OR2EB7L0HY27Heska Corp42805E306358333NSUSD353997171.048LongECCORPUSN1NNNICU Medical Inc5493003NRW7LS2QZRH60ICU Medical Inc44930G107166377NSUSD304070610.9LongECCORPUSN1NNNITT Inc549300KDSERHTJOXUS80ITT Inc45073V108561603NSUSD331626570.982LongECCORPUSN1NNNInsmed Inc529900Q55QAG41CCAC11Insmed Inc457669307752584NSUSD241880500.716LongECCORPUSN1NNNInsulet Corp549300TZTYD2PYN92D43Insulet Corp45784P101167104NSUSD395351351.17LongECCORPUSN1NNNIntegra LifeSciences HoldingsWB7IJCYY8YFTENS4O147Integra LifeSciences Holdings Corp457985208588712NSUSD277989810.823LongECCORPUSN1NNNIntelligent Systems CorpN/AIntelligent Systems Corp45816D100312179NSUSD121687370.36LongECCORPUSN1NNJ2 Global Inc5493008OJYGLHD7MXA18j2 Global Inc48123V102666154NSUSD461111801.365LongECCORPUSN1NNNK12 Inc254900BRWS3AFONWU277K12 Inc48273U102881738NSUSD232249790.687LongECCORPUSN1NNNLPL Financial Holdings Inc549300XROQ4Y8G2L7682LPL Financial Holdings Inc50212V100611495NSUSD468833221.388LongECCORPUSN1NNNLendingTree Inc549300ISY5YHUJZTON08LendingTree Inc52603B10774472NSUSD228547120.676LongECCORPUSN1NNLigand Pharmaceuticals Inc5493008K7TB0IKP37H79Ligand Pharmaceuticals Inc53220K504356860NSUSD340158951.007LongECCORPUSN1NNLivePerson Inc549300IN2P1JZ20G7N40LivePerson Inc538146101861476NSUSD447881371.326LongECCORPUSN1NNNLovesac Co/TheN/ALovesac Co54738L109753695NSUSD208848880.618LongECCORPUSN1NNMedallia Inc5493009P6W6C5BK60J60Medallia Inc584021109794705NSUSD217908110.645LongECCORPUSN1NNNMersana Therapeutics Inc529900VWD5VDZ7PVOS22Mersana Therapeutics Inc59045L106771811NSUSD143711210.425LongECCORPUSN1NNNMirati Therapeutics Inc529900GYRW59IDQV6N54Mirati Therapeutics Inc60468T105191740NSUSD318384270.942LongECCORPUSN1NNNMonarch Casino & Resort Inc549300AK5FF7KY11V997Monarch Casino & Resort Inc609027107292429NSUSD130423330.386LongECCORPUSN1NNNMontrose Environmental Group IN/AMontrose Environmental Group Inc615111101615062NSUSD146507770.434LongECCORPUSN1NNNMyoKardia Inc549300OLNNNZ1NI9UY54MyoKardia Inc62857M105230325NSUSD314002070.929LongECCORPUSN1NNNNapco Security Technologies In529900KV21J4YY9YST76Napco Security Technologies Inc63040210527185NSUSD6388480.019LongECCORPUSN1NNNNational Instruments Corp549300TP4OOPCYWDUP86National Instruments Corp636518102324815NSUSD115958960.343LongECCORPUSN1NNNNeoGenomics Inc549300KN62PPGHBVXB46NeoGenomics Inc64049M2091126569NSUSD415591301.23LongECCORPUSN1NNNNeurocrine Biosciences Inc549300FECER0XBN49756Neurocrine Biosciences Inc64125C109249814NSUSD240221140.711LongECCORPUSN1NNNNordson Corp14OS6Q5N55N95WM84M53Nordson Corp655663102171690NSUSD329335760.975LongECCORPUSN1NNNNovanta Inc549300NBPHPTUPTS8070Novanta Inc67000B104233402NSUSD245865670.728LongECCORPUSN1NNNOSI Systems IncDDX3MPX1Y8YD30050X33OSI Systems Inc671044105368209NSUSD285767000.846LongECCORPUSN1NNNOak Street Health IncN/AOak Street Health Inc67181A107193175NSUSD103232720.306LongECCORPUSN1NNON Semiconductor CorpZV20P4CNJVT8V1ZGJ064ON Semiconductor Corp6821891051660677NSUSD360200841.066LongECCORPUSN1NNNPTC Therapeutics Inc549300UJLWOIWFDGB318PTC Therapeutics Inc69366J200482557NSUSD225595400.668LongECCORPUSN1NNNPaylocity Holding Corp549300VH75FTB4DIEE07Paylocity Holding Corp70438V106341636NSUSD551468831.632LongECCORPUSN1NNNPhathom Pharmaceuticals IncN/APhathom Pharmaceuticals Inc71722W107317429NSUSD116401210.345LongECCORPUSN1NNNPhreesia Inc5493000HHF8NU4QOCG53Phreesia Inc71944F10661405NSUSD19729430.058LongECCORPUSN1NNNPrestige Consumer Healthcare IN/APrestige Consumer Healthcare Inc74112D101579347NSUSD210998180.625LongECCORPUSN1NNNProvidence Service Corp/The549300EFZIFW7F7I0Q28Providence Service Corp.74381510211029NSUSD10247040.03LongECCORPUSN1NNNRLI Corp529900AMTJE5ECN9PS55RLI Corp749607107337461NSUSD282556100.836LongECCORPUSN1NNNRealPage Inc5493001RSFZX4122A604RealPage Inc75606N109599910NSUSD345788121.023LongECCORPUSN1NNNRedfin Corp549300HXWD3JEMZU3O57Redfin Corp75737F1081032993NSUSD515773401.527LongECCORPUSN1NNNRepay Holdings CorpN/AREPAY HOLDINGS CORP76029L100751840NSUSD176682400.523LongECCORPUSN1NNNRetrophin IncN/ARetrophin Inc761299106630773NSUSD116440700.345LongECCORPUSN1NNNRexnord Corp549300AM3633XDFU1Q85Rexnord Corp76169B1021388999NSUSD414477301.227LongECCORPUSN1NNNRhythm Pharmaceuticals Inc529900JPNWXQ8DPLA551Rhythm Pharmaceuticals Inc76243J105664667NSUSD144033340.426LongECCORPUSN1NNNRogers CorpPW0CN80KNFR7RUOOKJ84Rogers Corp775133101140392NSUSD137668400.407LongECCORPUSN1NNNSPX CorpP761FCI0CPOG24I8JE10SPX Corp.78463510419391NSUSD8993550.027LongECCORPUSN1NNNSS&C Technologies Holdings Inc529900POY8H7NPPNKK71SS&C Technologies Holdings Inc78467J1001018420NSUSD616347781.824LongECCORPUSN1NNNSailpoint Technologies Holding529900809B15HNZH5H79SailPoint Technologies Holding Inc78781P1051066315NSUSD421940851.249LongECCORPUSN1NNNSealed Air CorpQ0M0CZB4YN6JZ65WXI09Sealed Air Corp81211K100524072NSUSD203392340.602LongECCORPUSN1NNNSensient Technologies CorpCMMZHMFZQG86EI7WZU92Sensient Technologies Corp81725T100828096NSUSD478142631.415LongECCORPUSN1NNNSeres Therapeutics Inc549300GMXBE5NUWISF13Seres Therapeutics Inc81750R102462469NSUSD130924970.388LongECCORPUSN1NNNServiceMaster Global Holdings549300GS10644JGL8R77ServiceMaster Global Holdings Inc81761R1091276060NSUSD508892731.506LongECCORPUSN1NNNShift4 Payments IncN/AShift4 Payments Inc - Class A82452J109100170NSUSD48442210.143LongECCORPUSN1NNNStandex International CorpRCX52GR0JW2LRRMH9Z54Standex International Corp854231107298823NSUSD176903220.524LongECCORPUSN1NNNSurmodics Inc549300H76KXCAAGUS878Surmodics Inc868873100350774NSUSD136486160.404LongECCORPUSN1NNNTrade Desk Inc/The549300GXPD31VT3E0P46Trade Desk Inc88339J10584411NSUSD437907391.296LongECCORPUSN1NNNThor Industries Inc254900XZO1228PDSG060THOR INDUSTRIES INC885160101178215NSUSD169767610.502LongECCORPUSN1NNNJanus Capital Group Inc8T2ZO87FU1KVPZIG9I59Janus Henderson Cash Liquidity FundN/A58457028NSUSD584628741.73LongECPFUSN2NNNJanus Henderson Cash Collateral FundN/AJanus Henderson Cash Collateral FundN/A72588179NSUSD725881792.148LongECPFUSN2NNTyler Technologies IncN/ATyler Technologies Inc90225210588179NSUSD307356720.91LongECCORPUSN1NNNValvoline Inc549300CMHPBEY6VPOT75Valvoline Inc92047W1012081812NSUSD396377001.173LongECCORPUSN1NNNVaxcyte Inc254900M7NMHTGASX8D95Vaxcyte Inc92243G1084976NSUSD2457150.007LongECCORPUSN1NNNVertex Inc549300QMYW171MP1PL50Vertex Inc - Class A92538J106175999NSUSD40479770.12LongECCORPUSN1NNNVisteon Corp549300MOVLYHRW4GGW78Visteon Corp92839U206266806NSUSD184683110.547LongECCORPUSN1NNNVonage Holdings Corp529900W7N0N4WLN1JY21Vonage Holdings Corp92886T2012070554NSUSD211817670.627LongECCORPUSN1NNNVroom IncN/AVroom Inc92918V109232328NSUSD120299440.356LongECCORPUSN1NNNWNS Holdings Ltd529900RM7QHUD3W9DM42WNS Holdings Ltd92932M101740818NSUSD473827191.402LongECCORPINN1NNNWEX IncT9M5IGROL1TL1G5OV478WEX Inc96208T104152626NSUSD212104350.628LongECCORPUSN1NNNWilliams-Sonoma Inc549300IARG6YWVTZ1I92Williams-Sonoma Inc969904101226695NSUSD205022960.607LongECCORPUSN1NNNZogenix Inc54930089H3HF4C32SK78Zogenix Inc98978L204651872NSUSD116880650.346LongECCORPUSN1NNNKnight Therapeutics Inc549300LNW93V3HRS7610Knight Therapeutics Inc4990531061841510NS80222010.237LongECCORPCAN1NNNEverarc Holdings Ltd213800HX5NY11VG7GW55Everarc Holdings Ltd999EF4TV01392863NSUSD181072190.536LongECCORPVGN1NNNOntex Group NV549300AQESC6JJOPW253Ontex Group NVB6S9X01091010514NS131965770.391LongECCORPBEN2NNNCimpress PLC549300X1IFD75XISOR81Cimpress PLCG2143T103153404NSUSD115298450.341LongECCORPIEN1NNNClarivate PLC549300WDHIIODXFO0R55Clarivate Analytics PLCG218101092470503NSUSD765608882.266LongECCORPGBN1NNNGates Industrial Corp PLCN/AGates Industrial Corp PLCG391081081567642NSUSD174321790.516LongECCORPUSN1NNNGTY Technology Holdings IncN/AGTY Technology Holdings Inc PPN/A2606NSUSD65610LongECCORPUSN2NNNICON PLC6354008SRQGEMC4XFI67ICON PLCG4705A100123846NSUSD236657320.7LongECCORPIEN1NNNManchester United Plc529900GWGZPE83MJOV59Manchester United PlcG5784H106965605NSUSD140398970.416LongECCORPGBN1NNMyovant Sciences LtdN/AMyovant Sciences LtdG637AM102759723NSUSD106741080.316LongECCORPGBN1NNNSTERIS PLCN/ASTERIS PLCG8473T100248715NSUSD438210961.297LongECCORPUSN1NNNJFrog LtdN/AJFROG LTDM6191J10028895NSUSD24459620.072LongECCORPILN1NNNKornit Digital Ltd5299006DPCQWP3VTFK34Kornit Digital LtdM6372Q113608331NSUSD394624321.168LongECCORPILN1NNNKensington Capital AcquisitionN/AKensington Capital Acquisition CorpN/A2036185NSUSD341060991.009LongEPCORPUSN2NNNAscendis Pharma A/S549300B66JN3W3J8GH73Ascendis Pharma A/S (ADR)04351P101104862NSUSD161823040.479LongCORPDKN1NNNBristol-Myers Squibb CoHLYYNH7UQUORYSJQCN42Bristol-Myers Squibb Co1101221571058489NSUSD23816000.07LongCORPUSN1NNNGW Pharmaceuticals PLC213800ISBN5CNUZMHZ39GW Pharmaceuticals PLC (ADR)36197T103168677NSUSD164207060.486LongCORPGBN1NNNice Ltd254900YTY4EF5Z4W0A51Nice Ltd (ADR)653656108464684NSUSD1054972093.123LongCORPILN1NNNOrchard Therapeutics plc21380056R45ONMT94Z50Orchard Therapeutics plc (ADR)68570P101618696NSUSD25428410.075LongCORPGBN1NNNN/AN/ASell CAD / Buy USDN/A1NCUSD-349966-0.01N/ADFEUSN2JPMorgan Chase Bank, National Association7H6GLXDRUGQFU57RNE9731654000CAD23425544USD2020-10-08-349966NNNN/AN/ASell CAD / Buy USDN/A1NCUSD-35112-0.001N/ADFEUSN2JPMorgan Chase Bank, National Association7H6GLXDRUGQFU57RNE973300000CAD2443538USD2020-10-08-35112NNNN/AN/ASell GBP / Buy USDN/A1NCUSD-36578-0.001N/ADFEUSN2Barclays Capital, Inc.AC28XWWI3WIBK28243191536800GBP1946122USD2020-10-08-36578NNNN/AN/ASell CAD / Buy USDN/A1NCUSD3318300.01N/ADFEUSN2Credit Suisse InternationalE58DKGMJYYYJLN8C386825561000CAD19534731USD2020-12-03331831NNNN/AN/ASell EUR / Buy USDN/A1NCUSD321580.001N/ADFEUSN2Credit Suisse InternationalE58DKGMJYYYJLN8C38682854000EUR3382407USD2020-12-0332158NNNN/AN/ASell CAD / Buy USDN/A1NCUSD1169770.003N/ADFEUSN2HSBC Securities (USA), Inc.CYYGQCGNHMHPSMRL3R9719638500CAD14873088USD2020-12-17116977NNNN/AN/ASell GBP / Buy USDN/A1NCUSD336430.001N/ADFEUSN2HSBC Securities (USA), Inc.CYYGQCGNHMHPSMRL3R979038600GBP11700287USD2020-12-1733643NNNN/AN/ASell EUR / Buy USDN/A1NCUSD318190.001N/ADFEUSN2HSBC Securities (USA), Inc.CYYGQCGNHMHPSMRL3R973467000EUR4103506USD2020-12-1731819NNNN/AN/ASell EUR / Buy USDN/A1NCUSD219720.001N/ADFEUSN2Citibank, National AssociationE57ODZWZ7FF32TWEFA762198000EUR2603331USD2020-12-1721972NNNN/AN/ASell CAD / Buy USDN/A1NCUSD872980.003N/ADFEUSN2Citibank, National AssociationE57ODZWZ7FF32TWEFA7613315000CAD10092014USD2020-12-1787298NNNN/AN/ASell CAD / Buy USDN/A1NCUSD-115610N/ADFEUSN2JPMorgan Chase Bank, National Association7H6GLXDRUGQFU57RNE973335000CAD2493378USD2020-10-08-11561NNNN/AN/AEVERARC HOLDINGS LTDN/A1392883NSUSD4178650.012N/ADECORPUSN1N/AN/ACallPurchasedEverarc Holdings LimitedEverarc Holdings Ltd139288312USD2022-12-01XXXX403936NNN2020-11-14JANUS INVESTMENT FUNDJESPER NERGAARDJESPER NERGAARDVP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER