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value="Sedol"/></identifiers><balance>82621</balance><units>NS</units><curCd>USD</curCd><valUSD>11858617</valUSD><pctVal>5.499</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Overseas Fund</name><lei>SHOQ1W1OPKO6Q4WZ8151</lei><title>Janus Henderson Overseas Fund - Class N Shares</title><cusip>47103D835</cusip><identifiers><isin value="US47103D8359"/><ticker value="JDINX US"/><other otherDesc="B8G4681" value="Sedol"/></identifiers><balance>142820</balance><units>NS</units><curCd>USD</curCd><valUSD>4673059</valUSD><pctVal>2.167</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Contrarian Fund</name><lei>05B6OC1W7N8NJQRIYS63</lei><title>Janus Henderson Contrarian Fund - Class N Shares</title><cusip>47103D207</cusip><identifiers><isin value="US47103D2071"/><ticker value="JCNNX US"/><other otherDesc="BHLSPK6" value="Sedol"/></identifiers><balance>494160</balance><units>NS</units><curCd>USD</curCd><valUSD>11138361</valUSD><pctVal>5.165</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Select Fund</name><lei>YTNSNBKH822ME5K53798</lei><title>Janus Henderson Global Select Fund - Class N Shares</title><cusip>47103D876</cusip><identifiers><isin value="US47103D8763"/><ticker value="JSLNX US"/><other otherDesc="BHLSPG2" value="Sedol"/></identifiers><balance>422036</balance><units>NS</units><curCd>USD</curCd><valUSD>6541565</valUSD><pctVal>3.034</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Small Cap Value Fund</name><lei>4JUN205P8U622DADVL73</lei><title>Janus Henderson Small Cap Value Fund - Class N Shares</title><cusip>47103D728</cusip><identifiers><isin value="US47103D7286"/><ticker value="JDSNX US"/><other otherDesc="B89DC87" value="Sedol"/></identifiers><balance>413507</balance><units>NS</units><curCd>USD</curCd><valUSD>7062701</valUSD><pctVal>3.275</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Mid Cap Value Fund</name><lei>DVD51L5R37HV8DO3EA61</lei><title>Janus Henderson Mid Cap Value Fund - Class N Shares</title><cusip>47103D744</cusip><identifiers><isin value="US47103D7443"/><ticker value="JDPNX US"/><other otherDesc="B756B53" value="Sedol"/></identifiers><balance>682351</balance><units>NS</units><curCd>USD</curCd><valUSD>9095734</valUSD><pctVal>4.218</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Triton Fund</name><lei>GKBLW4JP2S45CI5N6B55</lei><title>Janus Henderson Triton Fund - Class N Shares</title><cusip>47103D793</cusip><identifiers><isin value="US47103D7930"/><ticker value="JGMNX US"/><other otherDesc="B82VS68" value="Sedol"/></identifiers><balance>368095</balance><units>NS</units><curCd>USD</curCd><valUSD>11572900</valUSD><pctVal>5.367</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Bond Fund</name><lei>549300JVFLA561A67H59</lei><title>Janus Henderson Global Bond Fund - Class N Shares</title><cusip>47103D801</cusip><identifiers><isin value="US47103D8011"/><ticker value="JGLNX US"/><other otherDesc="BHLSPN9" value="Sedol"/></identifiers><balance>4686910</balance><units>NS</units><curCd>USD</curCd><valUSD>48509516</valUSD><pctVal>22.496</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Asia Equity Fund</name><lei>549300WZU4CKDFTFYJ24</lei><title>Janus Henderson Asia Equity Fund - Class N Shares</title><cusip>47103Y649</cusip><identifiers><isin value="US47103Y6499"/><ticker value="JAQNX US"/><other otherDesc="BG11821" value="Sedol"/></identifiers><balance>336466</balance><units>NS</units><curCd>USD</curCd><valUSD>4094785</valUSD><pctVal>1.899</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson International Value Fund</name><lei>549300KKBJM8SB6LR177</lei><title>Janus Henderson International Value Fund - Class N Shares</title><cusip>47103D579</cusip><identifiers><isin value="US47103D5793"/><ticker value="JIFNX US"/><other otherDesc="B9GQYZ7" value="Sedol"/></identifiers><balance>701806</balance><units>NS</units><curCd>USD</curCd><valUSD>6253090</valUSD><pctVal>2.9</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Multi-Sector I</name><lei>549300WARVMK66TM8315</lei><title>Janus Multi-Sector Income Fund Class N Shares</title><cusip>47103D496</cusip><identifiers><isin value="US47103D4960"/><ticker value="JMTNX US"/><other otherDesc="BK8G0L0" value="Sedol"/></identifiers><balance>1800631</balance><units>NS</units><curCd>USD</curCd><valUSD>17520143</valUSD><pctVal>8.125</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec></invstOrSecs><explntrNotes><explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/></explntrNotes><signature><ncom:dateSigned>2020-11-14</ncom:dateSigned><ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant><ncom:signature>JESPER NERGAARD</ncom:signature><ncom:signerName>JESPER NERGAARD</ncom:signerName><ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title></signature></nport:formData><nport:documents>XXXX</nport:documents></nport:edgarSubmission>
