<?xml version="1.0" encoding="UTF-8"?><nport:edgarSubmission xmlns="http://www.sec.gov/edgar/nport" xmlns:com="http://www.sec.gov/edgar/common" xmlns:ncom="http://www.sec.gov/edgar/nportcommon" xmlns:nport="http://www.sec.gov/edgar/nport" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.sec.gov/edgar/nport eis_NPORT_Filer.xsd "><nport:headerData><nport:submissionType>NPORT-P</nport:submissionType><nport:isConfidential>false</nport:isConfidential><nport:filerInfo><nport:filer><nport:issuerCredentials><nport:cik>0000277751</nport:cik><nport:ccc>XXXXXXXX</nport:ccc></nport:issuerCredentials></nport:filer><nport:seriesClassInfo><nport:seriesId>S000010482</nport:seriesId><nport:classId>C000028942</nport:classId><nport:classId>C000077714</nport:classId><nport:classId>C000077715</nport:classId><nport:classId>C000077716</nport:classId><nport:classId>C000077717</nport:classId><nport:classId>C000083528</nport:classId></nport:seriesClassInfo></nport:filerInfo></nport:headerData><nport:formData><genInfo><regName>JANUS INVESTMENT FUND</regName><regFileNumber>811-01879</regFileNumber><regCik>0000277751</regCik><regLei>W6UYDZFUOFJ4HQQ32382</regLei><regStreet1>151 DETROIT STREET</regStreet1><regCity>DENVER</regCity><regStateConditional regCountry="US" regState="US-CO"/><regZipOrPostalCode>80206</regZipOrPostalCode><regPhone>3033333863</regPhone><seriesName>Janus Henderson Global Allocation Fund - Moderate</seriesName><seriesId>S000010482</seriesId><seriesLei>54930051FC9ZUFNU6S35</seriesLei><repPdEnd>2020-06-30</repPdEnd><repPdDate>2019-09-30</repPdDate><isFinalFiling>N</isFinalFiling></genInfo><fundInfo><totAssets>233959291</totAssets><totLiabs>128637</totLiabs><netAssets>233830654</netAssets><assetsAttrMiscSec>0</assetsAttrMiscSec><assetsInvested>0</assetsInvested><amtPayOneYrBanksBorr>0</amtPayOneYrBanksBorr><amtPayOneYrCtrldComp>0</amtPayOneYrCtrldComp><amtPayOneYrOthAffil>0</amtPayOneYrOthAffil><amtPayOneYrOther>0</amtPayOneYrOther><amtPayAftOneYrBanksBorr>0</amtPayAftOneYrBanksBorr><amtPayAftOneYrCtrldComp>0</amtPayAftOneYrCtrldComp><amtPayAftOneYrOthAffil>0</amtPayAftOneYrOthAffil><amtPayAftOneYrOther>0</amtPayAftOneYrOther><delayDeliv>0</delayDeliv><standByCommit>0</standByCommit><liquidPref>0</liquidPref><cshNotRptdInCorD>11</cshNotRptdInCorD><isNonCashCollateral>N</isNonCashCollateral><returnInfo><monthlyTotReturns><monthlyTotReturn classId="C000077714" rtn1="0.0062" rtn2="-0.0023" rtn3="-0.0016"/><monthlyTotReturn classId="C000077715" rtn1="0.0063" rtn2="-0.0032" rtn3="-0.0024"/><monthlyTotReturn classId="C000083528" rtn1="0.0062" rtn2="-0.0023" rtn3="-0.0015"/><monthlyTotReturn classId="C000077716" rtn1="0.0062" rtn2="-0.0023" rtn3="-0.0015"/><monthlyTotReturn classId="C000077717" rtn1="0.0063" rtn2="-0.0031" rtn3="-0.0016"/><monthlyTotReturn classId="C000028942" rtn1="0.007" rtn2="-0.0023" rtn3="-0.0015"/></monthlyTotReturns><monthlyReturnCats><commodityContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></commodityContracts><creditContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></creditContracts><equityContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></equityContracts><foreignExchgContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></foreignExchgContracts><interestRtContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></interestRtContracts><otherContracts><mon1 netRealizedGain="0" netUnrealizedAppr="0"/><mon2 netRealizedGain="0" netUnrealizedAppr="0"/><mon3 netRealizedGain="0" netUnrealizedAppr="0"/><forwardCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></forwardCategory><futureCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></futureCategory><optionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></optionCategory><swaptionCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swaptionCategory><swapCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></swapCategory><warrantCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></warrantCategory><otherCategory><instrMon1 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon2 netRealizedGain="N/A" netUnrealizedAppr="N/A"/><instrMon3 netRealizedGain="N/A" netUnrealizedAppr="N/A"/></otherCategory></otherContracts></monthlyReturnCats><othMon1 netRealizedGain="60634" netUnrealizedAppr="1350356"/><othMon2 netRealizedGain="69294" netUnrealizedAppr="-726784"/><othMon3 netRealizedGain="3560390" netUnrealizedAppr="-3964371"/></returnInfo><mon1Flow redemption="-1804438.45" reinvestment="0" sales="1020920.35"/><mon2Flow redemption="-2283617.35" reinvestment="0" sales="599716.98"/><mon3Flow redemption="-2423844.66" reinvestment="0" sales="794510.83"/></fundInfo><invstOrSecs><invstOrSec><name>Janus Henderson Forty Fund</name><lei>549300RDSCGTT8PWPM78</lei><title>Janus Henderson Forty Fund - Class N Shares</title><cusip>47103D603</cusip><identifiers><isin value="US47103D6031"/><ticker value="JFRNX"/><other otherDesc="B8G4506" value="Sedol"/></identifiers><balance>66751</balance><units>NS</units><curCd>USD</curCd><valUSD>2593289</valUSD><pctVal>0.0111</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson US Managed Volatility Fund</name><lei>5493006DGCHLVTOMO890</lei><title>Janus Henderson U.S. Managed Volatility Fund - Class N Shares</title><cusip>47103E122</cusip><identifiers><isin value="US47103E1221"/><ticker value="JRSNX"/><other otherDesc="BJV8KM7" value="Sedol"/></identifiers><balance>881480</balance><units>NS</units><curCd>USD</curCd><valUSD>10330942</valUSD><pctVal>0.0442</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson International Managed Volatility Fund</name><lei>5493002OIR9CU80C2E05</lei><title>Janus Henderson International Managed Volatility Fund - Class N Shares</title><cusip>47103E155</cusip><identifiers><isin value="US47103E1551"/><ticker value="JMRNX"/><other otherDesc="BY9BPH3" value="Sedol"/></identifiers><balance>1322805</balance><units>NS</units><curCd>USD</curCd><valUSD>11468720</valUSD><pctVal>0.049</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Real Estate Fund</name><lei>A5263I2XDOP1IOXZPS12</lei><title>Janus Henderson Global Real Estate Fund - Class N Shares</title><cusip>47103Y623</cusip><identifiers><isin value="US47103Y6234"/><ticker value="JERNX"/><other otherDesc="BG11809" value="Sedol"/></identifiers><balance>221721</balance><units>NS</units><curCd>USD</curCd><valUSD>2900113</valUSD><pctVal>0.0124</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Large Cap Value Fund</name><lei>549300XP17M8QO3VV866</lei><title>Janus Henderson Large Cap Value Fund - Class N Shares</title><cusip>47103D751</cusip><identifiers><isin value="US47103D7518"/><ticker value="JPLNX"/><other otherDesc="B7W0374" value="Sedol"/></identifiers><balance>903083</balance><units>NS</units><curCd>USD</curCd><valUSD>12679290</valUSD><pctVal>0.0542</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Emerging Markets Fund</name><lei>549300OPPFBZE65UIL89</lei><title>Janus Henderson Emerging Markets Fund - Class N Shares</title><cusip>47103X716</cusip><identifiers><isin value="US47103X7167"/><ticker value="HEMRX"/><other otherDesc="BF334V9" value="Sedol"/></identifiers><balance>600530</balance><units>NS</units><curCd>USD</curCd><valUSD>5278660</valUSD><pctVal>0.0226</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Research Fund</name><lei>C0GC60LX4ZGTZPLZA692</lei><title>Janus Henderson Global Research Fund - Class N Shares</title><cusip>47103D777</cusip><identifiers><isin value="US47103D7773"/><ticker value="JDWNX"/><other otherDesc="BHLSPF1" value="Sedol"/></identifiers><balance>54577</balance><units>NS</units><curCd>USD</curCd><valUSD>4408214</valUSD><pctVal>0.0189</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Adaptive Global Allocation Fund</name><lei>549300HN0SRQIOHVMK11</lei><title>Janus Henderson Adaptive Global Allocation Fund - Class N Shares</title><cusip>47103D231</cusip><identifiers><isin value="US47103D2311"/><ticker value="JAGNX"/><other otherDesc="BYRY701" value="Sedol"/></identifiers><balance>1213414</balance><units>NS</units><curCd>USD</curCd><valUSD>12340422</valUSD><pctVal>0.0528</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Flexible Bond Fund</name><lei>C8DSHUK27T5OOEDPW945</lei><title>Janus Henderson Flexible Bond Fund - Class N Shares</title><cusip>47103D504</cusip><identifiers><isin value="US47103D5041"/><ticker value="JDFNX"/><other otherDesc="B796JF5" value="Sedol"/></identifiers><balance>597025</balance><units>NS</units><curCd>USD</curCd><valUSD>6310550</valUSD><pctVal>0.027</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Enterprise Fund</name><lei>TJ2Y3QQ1QRI8KQ84FR29</lei><title>Janus Henderson Enterprise Fund - Class N Shares</title><cusip>47103D405</cusip><identifiers><isin value="US47103D4051"/><ticker value="JDMNX"/><other otherDesc="B8F0PT4" value="Sedol"/></identifiers><balance>45340</balance><units>NS</units><curCd>USD</curCd><valUSD>6401551</valUSD><pctVal>0.0274</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Short-Term Bond Fund</name><lei>0IWXBJ4S82V64ZFH4582</lei><title>Janus Henderson Short-Term Bond Fund - Class N Shares</title><cusip>47103D819</cusip><identifiers><isin value="US47103D8193"/><ticker value="JSHNX"/><other otherDesc="B7FTRS7" value="Sedol"/></identifiers><balance>4446092</balance><units>NS</units><curCd>USD</curCd><valUSD>13427199</valUSD><pctVal>0.0574</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Overseas Fund</name><lei>SHOQ1W1OPKO6Q4WZ8151</lei><title>Janus Henderson Overseas Fund - Class N Shares</title><cusip>47103D835</cusip><identifiers><isin value="US47103D8359"/><ticker value="JDINX"/><other otherDesc="B8G4681" value="Sedol"/></identifiers><balance>485048</balance><units>NS</units><curCd>USD</curCd><valUSD>14852175</valUSD><pctVal>0.0635</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Contrarian Fund</name><lei>05B6OC1W7N8NJQRIYS63</lei><title>Janus Henderson Contrarian Fund - Class N Shares</title><cusip>47103D207</cusip><identifiers><isin value="US47103D2071"/><ticker value="JCNNX"/><other otherDesc="BHLSPK6" value="Sedol"/></identifiers><balance>450092</balance><units>NS</units><curCd>USD</curCd><valUSD>9757993</valUSD><pctVal>0.0417</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Select Fund</name><lei>YTNSNBKH822ME5K53798</lei><title>Janus Henderson Global Select Fund - Class N Shares</title><cusip>47103D876</cusip><identifiers><isin value="US47103D8763"/><ticker value="JSLNX"/><other otherDesc="BHLSPG2" value="Sedol"/></identifiers><balance>762903</balance><units>NS</units><curCd>USD</curCd><valUSD>11413027</valUSD><pctVal>0.0488</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Small Cap Value Fund</name><lei>4JUN205P8U622DADVL73</lei><title>Janus Henderson Small Cap Value Fund - Class N Shares</title><cusip>47103D728</cusip><identifiers><isin value="US47103D7286"/><ticker value="JDSNX"/><other otherDesc="B89DC87" value="Sedol"/></identifiers><balance>190209</balance><units>NS</units><curCd>USD</curCd><valUSD>4133239</valUSD><pctVal>0.0177</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Mid Cap Value Fund</name><lei>DVD51L5R37HV8DO3EA61</lei><title>Janus Henderson Mid Cap Value Fund - Class N Shares</title><cusip>47103D744</cusip><identifiers><isin value="US47103D7443"/><ticker value="JDPNX"/><other otherDesc="B756B53" value="Sedol"/></identifiers><balance>474447</balance><units>NS</units><curCd>USD</curCd><valUSD>7339700</valUSD><pctVal>0.0314</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Triton Fund</name><lei>GKBLW4JP2S45CI5N6B55</lei><title>Janus Henderson Triton Fund - Class N Shares</title><cusip>47103D793</cusip><identifiers><isin value="US47103D7930"/><ticker value="JGMNX"/><other otherDesc="B82VS68" value="Sedol"/></identifiers><balance>206652</balance><units>NS</units><curCd>USD</curCd><valUSD>6443421</valUSD><pctVal>0.0276</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Global Bond Fund</name><lei>549300JVFLA561A67H59</lei><title>Janus Henderson Global Bond Fund - Class N Shares</title><cusip>47103D801</cusip><identifiers><isin value="US47103D8011"/><ticker value="JGLNX"/><other otherDesc="BHLSPN9" value="Sedol"/></identifiers><balance>6086618</balance><units>NS</units><curCd>USD</curCd><valUSD>59101058</valUSD><pctVal>0.2528</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Asia Equity Fund</name><lei>549300WZU4CKDFTFYJ24</lei><title>Janus Henderson Asia Equity Fund - Class N Shares</title><cusip>47103Y649</cusip><identifiers><isin value="US47103Y6499"/><ticker value="JAQNX"/><other otherDesc="BG11821" value="Sedol"/></identifiers><balance>277722</balance><units>NS</units><curCd>USD</curCd><valUSD>2921631</valUSD><pctVal>0.0125</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson Diversified Alternatives Fund</name><lei>549300HL7VYXXV0TSE40</lei><title>Janus Henderson Diversified Alternatives Fund - Class N Shares</title><cusip>47103D652</cusip><identifiers><isin value="US47103D6528"/><ticker value="JDANX"/><other otherDesc="B7S7NY6" value="Sedol"/></identifiers><balance>2101436</balance><units>NS</units><curCd>USD</curCd><valUSD>20341904</valUSD><pctVal>0.087</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec><invstOrSec><name>Janus Henderson International Value Fund</name><lei>549300KKBJM8SB6LR177</lei><title>Janus Henderson International Value Fund - Class N Shares</title><cusip>47103D579</cusip><identifiers><isin value="US47103D5793"/><ticker value="JIFNX"/><other otherDesc="B9GQYZ7" value="Sedol"/></identifiers><balance>950625</balance><units>NS</units><curCd>USD</curCd><valUSD>9382672</valUSD><pctVal>0.0401</pctVal><payoffProfile>Long</payoffProfile><assetCat>EC</assetCat><issuerCat>RF</issuerCat><invCountry>US</invCountry><isRestrictedSec>N</isRestrictedSec><fairValLevel>1</fairValLevel><securityLending><isCashCollateral>N</isCashCollateral><isNonCashCollateral>N</isNonCashCollateral><isLoanByFund>N</isLoanByFund></securityLending></invstOrSec></invstOrSecs><signature><ncom:dateSigned>2019-11-14</ncom:dateSigned><ncom:nameOfApplicant>JESPER NERGAARD</ncom:nameOfApplicant><ncom:signature>JESPER NERGAARD</ncom:signature><ncom:signerName>JESPER NERGAARD</ncom:signerName><ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title></signature></nport:formData><nport:documents>XXXX</nport:documents></nport:edgarSubmission>
