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      id="t_1_fbdd777c_88fd_0ef8_a218_3f6caf1803a3">&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:20pt;font-family:times new roman;text-align:center;"&gt;Janus Investment Fund&lt;/div&gt;&lt;div style="margin-top:12pt;margin-bottom:0pt;font-size:14pt;font-family:times new roman;text-align:center;"&gt;Janus Henderson Global Allocation Fund &#x2013; Conservative &lt;/div&gt;&lt;div style="margin-top:12pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;Supplement dated September&#160;12, 2025 &lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;to Currently Effective Prospectuses &lt;/div&gt;&lt;div style="margin-top:36pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;"&gt;Effective immediately, the prospectuses for &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Conservative, Janus Henderson Global Allocation Fund &#x2013; Growth, &lt;/span&gt;and &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Moderate&lt;/span&gt; (the &#x201c;Funds&#x201d;) are amended as follows: &lt;/div&gt;&lt;div style="font-size:10pt;margin-top:0pt;margin-bottom:0pt;"&gt;&#160;&lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
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&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;1.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under &#x201c;&lt;span style="font-weight:bold"&gt;Principal Investment Strategies&lt;/span&gt;&#x201d; in the &lt;span style="font-weight:bold"&gt;Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following paragraph is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;In addition to investing in the underlying funds, the Fund may use derivatives, including forward foreign currency contracts to hedge risks associated with currency exposure. The Fund&#x2019;s exposure to derivatives will vary. &lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
&lt;tr style="page-break-inside:avoid"&gt;
&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;2.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under&lt;span style="font-weight:bold"&gt; &#x201c;Principal Investment Risks &#x2013; Fund-Level Risks&#x201d; &lt;/span&gt;in the&lt;span style="font-weight:bold"&gt; Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following risk factor is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;&lt;span style="font-weight:bold"&gt;&lt;span style="font-style:italic"&gt;Derivatives Risk. &lt;/span&gt;&lt;/span&gt;Derivatives can be volatile and involve risks in addition to the risks of the underlying referenced securities or asset. Gains or losses from a derivative investment can be substantially greater than the derivative&#x2019;s original cost and can therefore involve leverage. Leverage may cause the Fund to be more volatile than if it had not used leverage. Derivatives entail the risk that the counterparty may default on its payment obligations. If the counterparty to a derivative transaction defaults, the Fund may lose the net amount of the payments that it contractually is entitled to receive. Derivatives used for hedging purposes may reduce or eliminate gains or cause losses if the market moves in a manner different from that anticipated by portfolio management or if the cost of the derivative outweighs the benefit of the hedge. &lt;/div&gt;&lt;div style="margin-top:396pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;font-weight:bold;text-align:center;"&gt;Please retain this Supplement with your records. &lt;/div&gt;</oef:SupplementToProspectusTextBlock>
    <oef:SupplementToProspectusTextBlock
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      id="t_2_fc5194c7_988f_dd2f_7e8d_005b75925eb2">&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:20pt;font-family:times new roman;text-align:center;"&gt;Janus Investment Fund&lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:14pt;font-family:times new roman;text-align:center;"&gt;Janus Henderson Global Allocation Fund &#x2013; Growth &lt;/div&gt;&lt;div style="margin-top:12pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;Supplement dated September&#160;12, 2025 &lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;to Currently Effective Prospectuses &lt;/div&gt;&lt;div style="margin-top:36pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;"&gt;Effective immediately, the prospectuses for &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Conservative, Janus Henderson Global Allocation Fund &#x2013; Growth, &lt;/span&gt;and &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Moderate&lt;/span&gt; (the &#x201c;Funds&#x201d;) are amended as follows: &lt;/div&gt;&lt;div style="font-size:10pt;margin-top:0pt;margin-bottom:0pt;"&gt;&#160;&lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
&lt;tr style="page-break-inside:avoid"&gt;
&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;1.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under &#x201c;&lt;span style="font-weight:bold"&gt;Principal Investment Strategies&lt;/span&gt;&#x201d; in the &lt;span style="font-weight:bold"&gt;Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following paragraph is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;In addition to investing in the underlying funds, the Fund may use derivatives, including forward foreign currency contracts to hedge risks associated with currency exposure. The Fund&#x2019;s exposure to derivatives will vary. &lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
&lt;tr style="page-break-inside:avoid"&gt;
&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;2.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under&lt;span style="font-weight:bold"&gt; &#x201c;Principal Investment Risks &#x2013; Fund-Level Risks&#x201d; &lt;/span&gt;in the&lt;span style="font-weight:bold"&gt; Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following risk factor is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;&lt;span style="font-weight:bold"&gt;&lt;span style="font-style:italic"&gt;Derivatives Risk. &lt;/span&gt;&lt;/span&gt;Derivatives can be volatile and involve risks in addition to the risks of the underlying referenced securities or asset. Gains or losses from a derivative investment can be substantially greater than the derivative&#x2019;s original cost and can therefore involve leverage. Leverage may cause the Fund to be more volatile than if it had not used leverage. Derivatives entail the risk that the counterparty may default on its payment obligations. If the counterparty to a derivative transaction defaults, the Fund may lose the net amount of the payments that it contractually is entitled to receive. Derivatives used for hedging purposes may reduce or eliminate gains or cause losses if the market moves in a manner different from that anticipated by portfolio management or if the cost of the derivative outweighs the benefit of the hedge. &lt;/div&gt;&lt;div style="margin-top:396pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;font-weight:bold;text-align:center;"&gt;Please retain this Supplement with your records. &lt;/div&gt;</oef:SupplementToProspectusTextBlock>
    <oef:SupplementToProspectusTextBlock
      contextRef="S000010482Member"
      id="t_3_dd4cd783_2724_55b1_8483_eec4183ffb03">&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:20pt;font-family:times new roman;text-align:center;"&gt;Janus Investment Fund&lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:14pt;font-family:times new roman;text-align:center;"&gt;Janus Henderson Global Allocation Fund &#x2013; Moderate &lt;/div&gt;&lt;div style="margin-top:12pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;Supplement dated September&#160;12, 2025 &lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;to Currently Effective Prospectuses &lt;/div&gt;&lt;div style="margin-top:36pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;"&gt;Effective immediately, the prospectuses for &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Conservative, Janus Henderson Global Allocation Fund &#x2013; Growth, &lt;/span&gt;and &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Moderate&lt;/span&gt; (the &#x201c;Funds&#x201d;) are amended as follows: &lt;/div&gt;&lt;div style="font-size:10pt;margin-top:0pt;margin-bottom:0pt;"&gt;&#160;&lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
&lt;tr style="page-break-inside:avoid"&gt;
&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;1.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under &#x201c;&lt;span style="font-weight:bold"&gt;Principal Investment Strategies&lt;/span&gt;&#x201d; in the &lt;span style="font-weight:bold"&gt;Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following paragraph is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;In addition to investing in the underlying funds, the Fund may use derivatives, including forward foreign currency contracts to hedge risks associated with currency exposure. The Fund&#x2019;s exposure to derivatives will vary. &lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
&lt;tr style="page-break-inside:avoid"&gt;
&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;2.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under&lt;span style="font-weight:bold"&gt; &#x201c;Principal Investment Risks &#x2013; Fund-Level Risks&#x201d; &lt;/span&gt;in the&lt;span style="font-weight:bold"&gt; Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following risk factor is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;&lt;span style="font-weight:bold"&gt;&lt;span style="font-style:italic"&gt;Derivatives Risk. &lt;/span&gt;&lt;/span&gt;Derivatives can be volatile and involve risks in addition to the risks of the underlying referenced securities or asset. Gains or losses from a derivative investment can be substantially greater than the derivative&#x2019;s original cost and can therefore involve leverage. Leverage may cause the Fund to be more volatile than if it had not used leverage. Derivatives entail the risk that the counterparty may default on its payment obligations. If the counterparty to a derivative transaction defaults, the Fund may lose the net amount of the payments that it contractually is entitled to receive. Derivatives used for hedging purposes may reduce or eliminate gains or cause losses if the market moves in a manner different from that anticipated by portfolio management or if the cost of the derivative outweighs the benefit of the hedge. &lt;/div&gt;&lt;div style="margin-top:396pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;font-weight:bold;text-align:center;"&gt;Please retain this Supplement with your records. &lt;/div&gt;</oef:SupplementToProspectusTextBlock>
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      contextRef="DefaultContext"
      id="t_4_98349110_d2f3_6aba_90bc_55610ef03b35">&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:20pt;font-family:times new roman;text-align:center;"&gt;Janus Investment Fund&lt;/div&gt;&lt;div style="margin-top:12pt;margin-bottom:0pt;font-size:14pt;font-family:times new roman;text-align:center;"&gt;Janus Henderson Global Allocation Fund &#x2013; Conservative &lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:14pt;font-family:times new roman;text-align:center;"&gt;Janus Henderson Global Allocation Fund &#x2013; Growth &lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:14pt;font-family:times new roman;text-align:center;"&gt;Janus Henderson Global Allocation Fund &#x2013; Moderate &lt;/div&gt;&lt;div style="margin-top:12pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;Supplement dated September&#160;12, 2025 &lt;/div&gt;&lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:12pt;font-family:times new roman;text-align:center;"&gt;to Currently Effective Prospectuses &lt;/div&gt;&lt;div style="margin-top:36pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;"&gt;Effective immediately, the prospectuses for &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Conservative, Janus Henderson Global Allocation Fund &#x2013; Growth, &lt;/span&gt;and &lt;span style="font-weight:bold"&gt;Janus Henderson Global Allocation Fund &#x2013; Moderate&lt;/span&gt; (the &#x201c;Funds&#x201d;) are amended as follows: &lt;/div&gt;&lt;div style="font-size:10pt;margin-top:0pt;margin-bottom:0pt;"&gt;&#160;&lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
&lt;tr style="page-break-inside:avoid"&gt;
&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;1.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under &#x201c;&lt;span style="font-weight:bold"&gt;Principal Investment Strategies&lt;/span&gt;&#x201d; in the &lt;span style="font-weight:bold"&gt;Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following paragraph is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;In addition to investing in the underlying funds, the Fund may use derivatives, including forward foreign currency contracts to hedge risks associated with currency exposure. The Fund&#x2019;s exposure to derivatives will vary. &lt;/div&gt;
&lt;table cellpadding="0" cellspacing="0" style="border-collapse:collapse;font-family:times new roman;font-size:10pt;border-spacing:0px;width:100%"&gt;
&lt;tr style="page-break-inside:avoid"&gt;
&lt;td style="width:3%;vertical-align:top;text-align:left;"&gt;2.&lt;/td&gt;
&lt;td style="vertical-align:top;text-align:left;"&gt; &lt;div style="margin-top:0pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;text-align:left;"&gt;Under&lt;span style="font-weight:bold"&gt; &#x201c;Principal Investment Risks &#x2013; Fund-Level Risks&#x201d; &lt;/span&gt;in the&lt;span style="font-weight:bold"&gt; Fund Summary &lt;/span&gt;section of each Fund&#x2019;s&lt;span style="font-weight:bold"&gt; &lt;/span&gt;prospectuses, the following risk factor is added: &lt;/div&gt; &lt;/td&gt; &lt;/tr&gt; &lt;/table&gt;&lt;div style="margin-top:10pt;margin-bottom:0pt;margin-left:3%;font-size:10pt;font-family:times new roman;"&gt;&lt;span style="font-weight:bold"&gt;&lt;span style="font-style:italic"&gt;Derivatives Risk. &lt;/span&gt;&lt;/span&gt;Derivatives can be volatile and involve risks in addition to the risks of the underlying referenced securities or asset. Gains or losses from a derivative investment can be substantially greater than the derivative&#x2019;s original cost and can therefore involve leverage. Leverage may cause the Fund to be more volatile than if it had not used leverage. Derivatives entail the risk that the counterparty may default on its payment obligations. If the counterparty to a derivative transaction defaults, the Fund may lose the net amount of the payments that it contractually is entitled to receive. Derivatives used for hedging purposes may reduce or eliminate gains or cause losses if the market moves in a manner different from that anticipated by portfolio management or if the cost of the derivative outweighs the benefit of the hedge. &lt;/div&gt;&lt;div style="margin-top:396pt;margin-bottom:0pt;font-size:10pt;font-family:times new roman;font-weight:bold;text-align:center;"&gt;Please retain this Supplement with your records. &lt;/div&gt;</oef:SupplementToProspectusTextBlock>
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