Exhibit (a)(43)

JANUS INVESTMENT FUND

Certificate Redesignating

Janus Conservative Allocation Fund
Janus Growth Allocation Fund
Janus Moderate Allocation Fund

     The undersigned, being the Secretary of Janus Investment Fund, a Massachusetts business trust with transferable shares (the “Trust”), being duly authorized by a majority of the duly elected and qualified Trustees of the Trust acting pursuant to Sections 3.6 and 4.1 of the Trust’s Amended and Restated Agreement and Declaration of Trust dated March 18, 2003, as further amended from time to time (the “Declaration”), does hereby certify that, by affirmative vote of all members of the Trustees at a meeting held on November 8, 2012, (1) Janus Conservative Allocation Fund was redesignated as Janus Global Allocation Fund — Conservative; (2) Janus Growth Allocation Fund was redesignated as Janus Global Allocation Fund — Growth; and (3) Janus Moderate Allocation Fund was redesignated as Janus Global Allocation Fund — Moderate (each a “Fund”), with each such redesignation to be effective February 15, 2013.

     All of the current rights and preferences of each Fund remain in full force and effect, as set forth in the Declaration.

     The undersigned further certifies that she has been duly authorized by the Trustees of the Trust to take all necessary action to file a copy of this Certificate with the Secretary of the Commonwealth of Massachusetts and at any other place required by law or by the Declaration.

     IN WITNESS WHEREOF, I have hereunto set my hand as of the day and year set forth opposite my signature below.

Dated: February 11, 2013 /s/ Stephanie Grauerholz-Lofton   Stephanie Grauerholz-Lofton, Secretary

ACKNOWLEDGMENT

STATE OF COLORADO	)
	)	ss.
CITY AND COUNTY OF DENVER	)

     BEFORE ME, the undersigned authority, on this day personally appeared Stephanie Grauerholz-Lofton, Secretary of Janus Investment Fund, a Massachusetts business trust, who, after being first duly sworn, stated that she executed the foregoing document for the consideration therein expressed and in the capacity therein stated.

     SUBSCRIBED AND SWORN TO this 11^th day of February, 2013.

My Commission Expires:

6/16/2015 /s/ Lynn M. Donaldson   Notary Public — Lynn M. Donaldson