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    <isConfidential>false</isConfidential>
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          <ccc>XXXXXXXX</ccc>
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      <regFileNumber>811-01879</regFileNumber>
      <regCik>0000277751</regCik>
      <regLei>W6UYDZFUOFJ4HQQ32382</regLei>
      <regStreet1>151 Detroit Street</regStreet1>
      <regCity>Denver</regCity>
      <regStateConditional regCountry="US" regState="US-CO"/>
      <regZipOrPostalCode>80206</regZipOrPostalCode>
      <regPhone>303-333-3863</regPhone>
      <seriesName>Janus Henderson Global Select Fund</seriesName>
      <seriesId>S000010474</seriesId>
      <seriesLei>YTNSNBKH822ME5K53798</seriesLei>
      <repPdEnd>2026-09-30</repPdEnd>
      <repPdDate>2026-03-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
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      <totLiabs>8322516.460000000000</totLiabs>
      <netAssets>3039478652.610000000000</netAssets>
      <assetsAttrMiscSec>0.000000000000</assetsAttrMiscSec>
      <assetsInvested>0.000000000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.000000000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.000000000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.000000000000</amtPayOneYrOthAffil>
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      <amtPayAftOneYrBanksBorr>0.000000000000</amtPayAftOneYrBanksBorr>
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      <standByCommit>0.000000000000</standByCommit>
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      <isNonCashCollateral>N</isNonCashCollateral>
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      <mon2Flow redemption="25085971.50" reinvestment="1.97" sales="15581189.71"/>
      <mon3Flow redemption="30634122.81" reinvestment="0.00" sales="13974402.73"/>



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        <isRestrictedSec>Y</isRestrictedSec>

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        <name>Teck Resources Limited</name>
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        <currencyConditional curCd="CAD" exchangeRt="1.3909"/>
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        <invCountry>CA</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      </invstOrSec>
      <invstOrSec>
        <name>GE Vernova Inc.</name>
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        <title>GE Vernova Inc. COM</title>
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        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Glaukos Corp.</name>
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        <cusip>377322102</cusip>
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        <curCd>USD</curCd>
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        <assetCat>EC</assetCat>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      </invstOrSec>
      <invstOrSec>
        <name>LVMH Moet Hennessy Louis Vuitton SE</name>
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        <invCountry>FR</invCountry>

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        <fairValLevel>2</fairValLevel>
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        <name>Lennar Corp.</name>
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        <assetCat>EC</assetCat>
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        <invCountry>US</invCountry>

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        <name>Liberty Media Corp Del</name>
        <lei>549300ZKUTPIBZLWLL89</lei>
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        <cusip>531229755</cusip>
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          <ticker value="FWONK"/>
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        <curCd>USD</curCd>
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        <assetCat>EC</assetCat>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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        <name>Rheinmetall AG</name>
        <lei>5299001OU9CSE29O6S05</lei>
        <title>Rheinmetall AG NPV</title>
        <cusip>D65111102</cusip>
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        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>DE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Microsoft Corp.</name>
        <lei>INR2EJN1ERAN0W5ZP974</lei>
        <title>Microsoft Corp. COM USD0.00000625</title>
        <cusip>594918104</cusip>
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          <ticker value="MSFT"/>
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        <curCd>USD</curCd>
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        <assetCat>EC</assetCat>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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        <name>Monster Beverage Corp</name>
        <lei>52990090AP0E7HCB6F33</lei>
        <title>Monster Beverage Corp COM USD0.005</title>
        <cusip>61174X109</cusip>
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          <ticker value="MNST"/>
          <other otherDesc="SEDOL" value="BZ07BW4"/>
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        <units>NS</units>
        <curCd>USD</curCd>
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        <pctVal>1.0039672302</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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        <name>Netflix, Inc.</name>
        <lei>549300Y7VHGU0I7CE873</lei>
        <title>Netflix, Inc. COM USD0.001</title>
        <cusip>64110L106</cusip>
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          <ticker value="NFLX"/>
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        <assetCat>EC</assetCat>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isLoanByFund>N</isLoanByFund>
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      <invstOrSec>
        <name>Resona Holdings Inc.</name>
        <lei>52990067JE75XZCYYT81</lei>
        <title>Resona Holdings Inc. NPV</title>
        <cusip>J6448E106</cusip>
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        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>JP</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

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      <invstOrSec>
        <name>NVIDIA Corp.</name>
        <lei>549300S4KLFTLO7GSQ80</lei>
        <title>NVIDIA Corp. COM USD0.001</title>
        <cusip>67066G104</cusip>
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          <ticker value="NVDA"/>
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        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>The Progressive Corporation</name>
        <lei>529900TACNVLY9DCR586</lei>
        <title>The Progressive Corporation COM USD1.00</title>
        <cusip>743315103</cusip>
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          <ticker value="PGR"/>
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        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>The TJX Companies Inc.</name>
        <lei>V167QI9I69W364E2DY52</lei>
        <title>The TJX Companies Inc. COM USD1</title>
        <cusip>872540109</cusip>
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          <other otherDesc="SEDOL" value="2989301"/>
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        <curCd>USD</curCd>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
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      <invstOrSec>
        <name>T-Mobile US Inc</name>
        <lei>549300QHIJYOHPACPG31</lei>
        <title>T-Mobile US Inc COM USD0.00001</title>
        <cusip>872590104</cusip>
        <identifiers>
          <isin value="US8725901040"/>
          <ticker value="TMUS"/>
          <other otherDesc="SEDOL" value="B94Q9V0"/>
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        <balance>333695</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>70085960.85</valUSD>
        <pctVal>2.3058546830</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="895"/>
        </identifiers>
        <balance>50740112.6839</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>50740112.68</valUSD>
        <pctVal>1.6693689438</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Rentokil Initial PLC</name>
        <lei>549300VN4WV7Z6T14K68</lei>
        <title>Rentokil Initial PLC ORD GBP0.01</title>
        <cusip>G7494G105</cusip>
        <identifiers>
          <isin value="GB00B082RF11"/>
          <ticker value="RKLIF"/>
          <other otherDesc="SEDOL" value="B082RF1"/>
        </identifiers>
        <balance>6812621</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>42351936.4</valUSD>
        <pctVal>1.3933947641</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Samsonite Group SA</name>
        <lei>549300UULS5HYFJSEL12</lei>
        <title>Samsonite Group SA USD0.01</title>
        <cusip>L80308106</cusip>
        <identifiers>
          <isin value="LU0633102719"/>
          <ticker value="SMSOF"/>
          <other otherDesc="SEDOL" value="B4Q1532"/>
        </identifiers>
        <balance>21042000</balance>
        <units>NS</units>
        <currencyConditional curCd="HKD" exchangeRt="7.841"/>
        <valUSD>39683649.29</valUSD>
        <pctVal>1.3056071065</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>HK</invCountry>

        <isRestrictedSec>Y</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Tencent Holdings Ltd.</name>
        <lei>254900N4SLUMW4XUYY11</lei>
        <title>Tencent Holdings Ltd. HKD0.00002</title>
        <cusip>G87572163</cusip>
        <identifiers>
          <isin value="KYG875721634"/>
          <ticker value="TCTZF"/>
          <other otherDesc="SEDOL" value="BMMV2K8"/>
        </identifiers>
        <balance>658500</balance>
        <units>NS</units>
        <currencyConditional curCd="HKD" exchangeRt="7.841"/>
        <valUSD>41573418.36</valUSD>
        <pctVal>1.3677812254</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>HK</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Japan Post Bank Co Ltd.</name>
        <lei>5493006JVO6N2HC2XZ76</lei>
        <title>Japan Post Bank Co Ltd. NPV</title>
        <cusip>J2800C101</cusip>
        <identifiers>
          <isin value="JP3946750001"/>
          <ticker value="JPSTF"/>
          <other otherDesc="SEDOL" value="BYT8165"/>
        </identifiers>
        <balance>2847900</balance>
        <units>NS</units>
        <currencyConditional curCd="JPY" exchangeRt="158.69"/>
        <valUSD>46858493.3</valUSD>
        <pctVal>1.5416621946</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>JP</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Spotify Technology S.A.</name>
        <lei>549300B4X0JHWV0DTD60</lei>
        <title>Spotify Technology S.A. COM EUR0.000625</title>
        <cusip>L8681T102</cusip>
        <identifiers>
          <isin value="LU1778762911"/>
          <ticker value="SPOT"/>
          <other otherDesc="SEDOL" value="BFZ1K46"/>
        </identifiers>
        <balance>109386</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>53042365.26</valUSD>
        <pctVal>1.7451139265</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Ferguson Enterprises Inc</name>
        <lei>2138003JYQMRP3SLX189</lei>
        <title>Ferguson Enterprises Inc COMMON STOCK NEW</title>
        <cusip>31488V107</cusip>
        <identifiers>
          <isin value="US31488V1070"/>
          <ticker value="FERG"/>
          <other otherDesc="SEDOL" value="BS6VHW3"/>
        </identifiers>
        <balance>413270</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>96399360.2</valUSD>
        <pctVal>3.1715754976</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Freeport-McMoRan Inc.</name>
        <lei>549300IRDTHJQ1PVET45</lei>
        <title>Freeport-McMoRan Inc. COM USD0.10</title>
        <cusip>35671D857</cusip>
        <identifiers>
          <isin value="US35671D8570"/>
          <ticker value="FCX"/>
          <other otherDesc="SEDOL" value="2352118"/>
        </identifiers>
        <balance>775180</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>45565080.4</valUSD>
        <pctVal>1.4991084198</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Erste Group Bank AG</name>
        <lei>PQOH26KWDF7CG10L6792</lei>
        <title>Erste Group Bank AG NPV</title>
        <cusip>A19494102</cusip>
        <identifiers>
          <isin value="AT0000652011"/>
          <ticker value="EBKOF"/>
          <other otherDesc="SEDOL" value="5289837"/>
        </identifiers>
        <balance>784239</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>84730184.24</valUSD>
        <pctVal>2.7876551845</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>AT</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Eli Lilly &amp; Co.</name>
        <lei>FRDRIPF3EKNDJ2CQJL29</lei>
        <title>Eli Lilly &amp; Co. COM NPV</title>
        <cusip>532457108</cusip>
        <identifiers>
          <isin value="US5324571083"/>
          <ticker value="LLY"/>
          <other otherDesc="SEDOL" value="2516152"/>
        </identifiers>
        <balance>62848</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>57805704.96</valUSD>
        <pctVal>1.9018296085</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Eaton Corporation PLC</name>
        <lei>549300VDIGTMXUNT7H71</lei>
        <title>Eaton Corporation PLC COM USD0.01</title>
        <cusip>G29183103</cusip>
        <identifiers>
          <isin value="IE00B8KQN827"/>
          <ticker value="ETN"/>
          <other otherDesc="SEDOL" value="B8KQN82"/>
        </identifiers>
        <balance>131027</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>46864427.09</valUSD>
        <pctVal>1.5418574185</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ConocoPhillips</name>
        <lei>WPTL2Z3FIYTHSP5V2253</lei>
        <title>ConocoPhillips COM USD0.01</title>
        <cusip>20825C104</cusip>
        <identifiers>
          <isin value="US20825C1045"/>
          <ticker value="COP"/>
          <other otherDesc="SEDOL" value="2685717"/>
        </identifiers>
        <balance>886987</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>117082284</valUSD>
        <pctVal>3.8520515319</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Deere &amp; Company</name>
        <lei>PWFTNG3EI0Y73OXWDH08</lei>
        <title>Deere &amp; Company COM USD1</title>
        <cusip>244199105</cusip>
        <identifiers>
          <isin value="US2441991054"/>
          <ticker value="DE"/>
          <other otherDesc="SEDOL" value="2261203"/>
        </identifiers>
        <balance>106027</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>59725009.1</valUSD>
        <pctVal>1.9649754424</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Daiichi Life Group Inc.</name>
        <lei>549300ZUFXI7JXZVTZ25</lei>
        <title>Daiichi Life Group Inc. NPV</title>
        <cusip>J09748112</cusip>
        <identifiers>
          <isin value="JP3476480003"/>
          <ticker value="DCNSF"/>
          <other otherDesc="SEDOL" value="B601QS4"/>
        </identifiers>
        <balance>4690100</balance>
        <units>NS</units>
        <currencyConditional curCd="JPY" exchangeRt="158.69"/>
        <valUSD>42862542.54</valUSD>
        <pctVal>1.4101938996</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>JP</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <loanByFundCondition isLoanByFund="Y" loanVal="31316596.220000000000"/>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Contemporary Amperex Technology Co Ltd.</name>
        <lei>300300KINDY1K0E9I032</lei>
        <title>Contemporary Amperex Technology Co Ltd. 'A' CNY1</title>
        <cusip>Y1R48E105</cusip>
        <identifiers>
          <isin value="CNE100003662"/>
          <ticker value="300750"/>
          <other otherDesc="SEDOL" value="BHQPSY7"/>
        </identifiers>
        <balance>949726</balance>
        <units>NS</units>
        <currencyConditional curCd="CNY" exchangeRt="6.8874"/>
        <valUSD>55834798.64</valUSD>
        <pctVal>1.8369860434</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CN</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Taiwan Semiconductor Manufacturing Co., Ltd.</name>
        <lei>549300KB6NK5SBD14S87</lei>
        <title>Taiwan Semiconductor Manufacturing Co., Ltd. TWD10</title>
        <cusip>Y84629107</cusip>
        <identifiers>
          <isin value="TW0002330008"/>
          <ticker value="TSMWF"/>
          <other otherDesc="SEDOL" value="6889106"/>
        </identifiers>
        <balance>3597000</balance>
        <units>NS</units>
        <currencyConditional curCd="TWD" exchangeRt="31.965"/>
        <valUSD>202814166.24</valUSD>
        <pctVal>6.6726629603</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>TW</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vaxcyte Inc</name>
        <lei>254900M7NMHTGASX8D95</lei>
        <title>Vaxcyte Inc COM</title>
        <cusip>92243G108</cusip>
        <identifiers>
          <isin value="US92243G1085"/>
          <ticker value="PCVX"/>
          <other otherDesc="SEDOL" value="BKPVGH6"/>
        </identifiers>
        <balance>503822</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>29277096.42</valUSD>
        <pctVal>0.9632275718</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vistra Corp</name>
        <lei>549300KP43CPCUJOOG15</lei>
        <title>Vistra Corp COM USD0.01</title>
        <cusip>92840M102</cusip>
        <identifiers>
          <isin value="US92840M1027"/>
          <ticker value="VST"/>
          <other otherDesc="SEDOL" value="BZ8VJQ8"/>
        </identifiers>
        <balance>275786</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>41458909.38</valUSD>
        <pctVal>1.3640138365</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Unilever PLC</name>
        <lei>549300MKFYEKVRWML317</lei>
        <title>Unilever PLC ORD GBP0.035</title>
        <cusip>G92087348</cusip>
        <identifiers>
          <isin value="GB00BVZK7T90"/>
          <ticker value="UNLYF"/>
          <other otherDesc="SEDOL" value="BVZK7T9"/>
        </identifiers>
        <balance>1142749</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>64060455.61</valUSD>
        <pctVal>2.1076132762</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Alphabet Inc.</name>
        <lei>5493006MHB84DD0ZWV18</lei>
        <title>Alphabet Inc. CAP STK USD0.001 CL A</title>
        <cusip>02079K305</cusip>
        <identifiers>
          <isin value="US02079K3059"/>
          <ticker value="GOOGL"/>
          <other otherDesc="SEDOL" value="BYVY8G0"/>
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        <balance>483274</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>138970271.44</valUSD>
        <pctVal>4.5721746169</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Amazon.com, Inc.</name>
        <lei>ZXTILKJKG63JELOEG630</lei>
        <title>Amazon.com, Inc. COM USD0.01</title>
        <cusip>023135106</cusip>
        <identifiers>
          <isin value="US0231351067"/>
          <ticker value="AMZN"/>
          <other otherDesc="SEDOL" value="2000019"/>
        </identifiers>
        <balance>630841</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>131385255.07</valUSD>
        <pctVal>4.3226247027</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>BAE Systems PLC</name>
        <lei>8SVCSVKSGDWMW2QHOH83</lei>
        <title>BAE Systems PLC ORD GBP0.025</title>
        <cusip>G06940103</cusip>
        <identifiers>
          <isin value="GB0002634946"/>
          <ticker value="BAESF"/>
          <other otherDesc="SEDOL" value="0263494"/>
        </identifiers>
        <balance>4311387</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>125481017.79</valUSD>
        <pctVal>4.1283730577</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Argenx SE</name>
        <lei>7245009C5FZE6G9ODQ71</lei>
        <title>Argenx SE SPON ADR EACH REP 1 ORD SHS</title>
        <cusip>04016X101</cusip>
        <identifiers>
          <isin value="US04016X1019"/>
          <ticker value="ARGX"/>
          <other otherDesc="SEDOL" value="BDVLM39"/>
        </identifiers>
        <balance>52614</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>38421373.5</valUSD>
        <pctVal>1.2640777545</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="GDR"/>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Ascendis Pharma A/S</name>
        <lei>549300B66JN3W3J8GH73</lei>
        <title>Ascendis Pharma A/S SPON ADR EACH REP 1 ORD SHS</title>
        <cusip>04351P101</cusip>
        <identifiers>
          <isin value="US04351P1012"/>
          <ticker value="ASND"/>
          <other otherDesc="SEDOL" value="BV9G6B8"/>
        </identifiers>
        <balance>130455</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>29838972.15</valUSD>
        <pctVal>0.9817134963</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="GDR"/>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>AstraZeneca PLC</name>
        <lei>PY6ZZQWO2IZFZC3IOL08</lei>
        <title>AstraZeneca PLC ORD USD0.25</title>
        <cusip>G0593M107</cusip>
        <identifiers>
          <isin value="GB0009895292"/>
          <ticker value="AZN"/>
          <other otherDesc="SEDOL" value="0989529"/>
        </identifiers>
        <balance>258862</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>50552737.95</valUSD>
        <pctVal>1.6632042441</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Boston Scientific Corporation</name>
        <lei>Y6ZDD9FP4P8JSSJMW954</lei>
        <title>Boston Scientific Corporation COM USD0.01</title>
        <cusip>101137107</cusip>
        <identifiers>
          <isin value="US1011371077"/>
          <ticker value="BSX"/>
          <other otherDesc="SEDOL" value="2113434"/>
        </identifiers>
        <balance>435781</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>27345257.75</valUSD>
        <pctVal>0.8996693471</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Gallagher Aj &amp;Co</name>
        <lei>54930049QLLMPART6V29</lei>
        <title>Gallagher Aj &amp;Co COM USD1.00</title>
        <cusip>363576109</cusip>
        <identifiers>
          <isin value="US3635761097"/>
          <ticker value="AJG"/>
          <other otherDesc="SEDOL" value="2359506"/>
        </identifiers>
        <balance>287028</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>62164524.24</valUSD>
        <pctVal>2.0452364154</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Banco Bilbao Vizcaya Argentaria SA</name>
        <lei>K8MS7FD7N5Z2WQ51AZ71</lei>
        <title>Banco Bilbao Vizcaya Argentaria SA EUR0.49</title>
        <cusip>E11805103</cusip>
        <identifiers>
          <isin value="ES0113211835"/>
          <ticker value="BBVXF"/>
          <other otherDesc="SEDOL" value="5501906"/>
        </identifiers>
        <balance>3777753</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>82443399.32</valUSD>
        <pctVal>2.7124190936</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>ES</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>3M Company</name>
        <lei>LUZQVYP4VS22CLWDAR65</lei>
        <title>3M Company COM USD0.01</title>
        <cusip>88579Y101</cusip>
        <identifiers>
          <isin value="US88579Y1010"/>
          <ticker value="MMM"/>
          <other otherDesc="SEDOL" value="2595708"/>
        </identifiers>
        <balance>195449</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>28385058.27</valUSD>
        <pctVal>0.9338791784</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>AIA Group Ltd, Hong Kong</name>
        <lei>ZP5ILWVSYE4LJGMMVD57</lei>
        <title>AIA Group Ltd, Hong Kong NPV</title>
        <cusip>Y002A1105</cusip>
        <identifiers>
          <isin value="HK0000069689"/>
          <ticker value="AAIGF"/>
          <other otherDesc="SEDOL" value="B4TX8S1"/>
        </identifiers>
        <balance>3615000</balance>
        <units>NS</units>
        <currencyConditional curCd="HKD" exchangeRt="7.841"/>
        <valUSD>40611400.45</valUSD>
        <pctVal>1.3361304714</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>HK</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ASML Holding N.V.</name>
        <lei>724500Y6DUVHQD6OXN27</lei>
        <title>ASML Holding N.V. EUR0.09</title>
        <cusip>N07059202</cusip>
        <identifiers>
          <isin value="NL0010273215"/>
          <ticker value="ASMLF"/>
          <other otherDesc="SEDOL" value="B929F46"/>
        </identifiers>
        <balance>71769</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>94983378.58</valUSD>
        <pctVal>3.1249891654</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>NL</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>Royal Bank of Canada</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="RBC SL TD"/>
        </identifiers>
        <balance>6609892</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>6609892</valUSD>
        <pctVal>0.2174679527</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2026-04-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>3.65</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <cashCollateralCondition cashCollateralVal="6609892.000000000000" isCashCollateral="Y"/>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Collateral Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Collateral Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="896"/>
        </identifiers>
        <balance>26439568</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>26439568</valUSD>
        <pctVal>0.8698718110</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <cashCollateralCondition cashCollateralVal="26439568.000000000000" isCashCollateral="Y"/>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Japan Yen Cash Balance</name>
        <lei>N/A</lei>
        <title>Japan Yen Cash Balance</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="JPY Cash"/>
        </identifiers>
        <balance>3165</balance>
        <units>OU</units>
        <descOthUnits>Currency</descOthUnits>
        <currencyConditional curCd="JPY" exchangeRt="158.69"/>
        <valUSD>3165</valUSD>
        <pctVal>0.0001041297</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Foreign Currencies" issuerCat="OTHER"/>
        <invCountry>JP</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Canada Dollar Cash Balance</name>
        <lei>N/A</lei>
        <title>Canada Dollar Cash Balance</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="CAD Cash"/>
        </identifiers>
        <balance>90432.35</balance>
        <units>OU</units>
        <descOthUnits>Currency</descOthUnits>
        <currencyConditional curCd="CAD" exchangeRt="1.3909"/>
        <valUSD>90432.35</valUSD>
        <pctVal>0.0029752586</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Foreign Currencies" issuerCat="OTHER"/>
        <invCountry>CA</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-05-15</ncom:dateSigned>
      <ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant>
      <ncom:signature>JESPER NERGAARD</ncom:signature>
      <ncom:signerName>JESPER NERGAARD</ncom:signerName>
      <ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
