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      <regStreet1>151 Detroit Street</regStreet1>
      <regCity>Denver</regCity>
      <regStateConditional regCountry="US" regState="US-CO"/>
      <regZipOrPostalCode>80206</regZipOrPostalCode>
      <regPhone>303-333-3863</regPhone>
      <seriesName>Janus Henderson Global Allocation Fund - Moderate</seriesName>
      <seriesId>S000010482</seriesId>
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      <repPdEnd>2026-06-30</repPdEnd>
      <repPdDate>2026-03-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
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      <totLiabs>97263.010000000000</totLiabs>
      <netAssets>201378628.050000000000</netAssets>
      <assetsAttrMiscSec>0.000000000000</assetsAttrMiscSec>
      <assetsInvested>0.000000000000</assetsInvested>
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        <othMon3 netRealizedGain="5272200" netUnrealizedAppr="N/A"/>
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        <name>Janus Investment Fund</name>
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        <isRestrictedSec>N</isRestrictedSec>

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        <name>Janus Investment Fund</name>
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        <isRestrictedSec>N</isRestrictedSec>

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        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
        <title>Janus Investment Fund EURPN FCS FD N</title>
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          <isin value="US47103X5914"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
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          <isin value="US47103D8276"/>
          <ticker value="JRANX"/>
          <other otherDesc="Internal ID" value="1448"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
        <title>Janus Investment Fund FLEXIBLE N</title>
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          <isin value="US47103D5041"/>
          <ticker value="JDFNX"/>
          <other otherDesc="SEDOL" value="B796JF5"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
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        <isRestrictedSec>N</isRestrictedSec>

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      <invstOrSec>
        <name>Janus Investment Fund</name>
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          <isin value="US47103D8359"/>
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          <other otherDesc="SEDOL" value="B8G4681"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
        <title>Janus Investment Fund DVLPD WRLD BND N</title>
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          <isin value="US47103Y8214"/>
          <ticker value="HFARX"/>
          <other otherDesc="SEDOL" value="BF33SV7"/>
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        <curCd>USD</curCd>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Trust</name>
        <lei>5493000860OXIC4B5K91</lei>
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        <identifiers>
          <isin value="US46435G6724"/>
          <ticker value="IAGG"/>
          <other otherDesc="SEDOL" value="BYQKX57"/>
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        <balance>506416</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>25341056.64</valUSD>
        <pctVal>12.5837865147</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Detroit Street Trust</name>
        <lei>5493003MM7OCK5UJW595</lei>
        <title>Janus Detroit Street Trust HENDERSON EMERGING MARKETS DEBT HARD CURRENCY ETF</title>
        <cusip>47103U738</cusip>
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          <isin value="US47103U7384"/>
          <ticker value="JEMB"/>
          <other otherDesc="SEDOL" value="BT6MK79"/>
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        <balance>132943</balance>
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        <curCd>USD</curCd>
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        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
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        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="895"/>
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        <balance>3682831.39135</balance>
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        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>Vanguard Index Funds</name>
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          <ticker value="VTV"/>
          <other otherDesc="SEDOL" value="2353638"/>
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        <balance>28136</balance>
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        <curCd>USD</curCd>
        <valUSD>5520283.2</valUSD>
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        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>iShares Trust</name>
        <lei>5493000860OXIC4B5K91</lei>
        <title>iShares Trust RUSSELL 2000 VALUE ETF</title>
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          <isin value="US4642876308"/>
          <ticker value="IWN"/>
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        <curCd>USD</curCd>
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        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>Ishares Inc</name>
        <lei>549300MGJZCNMJLBAJ67</lei>
        <title>Ishares Inc CORE MSCI EMERGING MKTS ETF</title>
        <cusip>46434G103</cusip>
        <identifiers>
          <isin value="US46434G1031"/>
          <ticker value="IEMG"/>
          <other otherDesc="SEDOL" value="B8NDCB6"/>
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        <balance>295396</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>20603871</valUSD>
        <pctVal>10.2314089631</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-05-15</ncom:dateSigned>
      <ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant>
      <ncom:signature>JESPER NERGAARD</ncom:signature>
      <ncom:signerName>JESPER NERGAARD</ncom:signerName>
      <ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
