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    <isConfidential>false</isConfidential>
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          <ccc>XXXXXXXX</ccc>
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      <regFileNumber>811-01879</regFileNumber>
      <regCik>0000277751</regCik>
      <regLei>W6UYDZFUOFJ4HQQ32382</regLei>
      <regStreet1>151 Detroit Street</regStreet1>
      <regCity>Denver</regCity>
      <regStateConditional regCountry="US" regState="US-CO"/>
      <regZipOrPostalCode>80206</regZipOrPostalCode>
      <regPhone>303-333-3863</regPhone>
      <seriesName>Janus Henderson Global Allocation Fund - Growth</seriesName>
      <seriesId>S000010481</seriesId>
      <seriesLei>549300099CS4YRT0YV31</seriesLei>
      <repPdEnd>2026-06-30</repPdEnd>
      <repPdDate>2026-03-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
    </genInfo>
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      <totLiabs>108107.040000000000</totLiabs>
      <netAssets>246258957.680000000000</netAssets>
      <assetsAttrMiscSec>0.000000000000</assetsAttrMiscSec>
      <assetsInvested>0.000000000000</assetsInvested>
      <amtPayOneYrBanksBorr>0.000000000000</amtPayOneYrBanksBorr>
      <amtPayOneYrCtrldComp>0.000000000000</amtPayOneYrCtrldComp>
      <amtPayOneYrOthAffil>0.000000000000</amtPayOneYrOthAffil>
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      <amtPayAftOneYrOther>0.000000000000</amtPayAftOneYrOther>
      <delayDeliv>0.000000000000</delayDeliv>
      <standByCommit>0.000000000000</standByCommit>
      <liquidPref>0.000000000000</liquidPref>
      <cshNotRptdInCorD>0.000000000000</cshNotRptdInCorD>
      <isNonCashCollateral>N</isNonCashCollateral>
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        <othMon2 netRealizedGain="184023" netUnrealizedAppr="1787244"/>
        <othMon3 netRealizedGain="7713636" netUnrealizedAppr="N/A"/>
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      <mon2Flow redemption="3199279.92" reinvestment="0.00" sales="1310828.92"/>
      <mon3Flow redemption="3418378.55" reinvestment="0.00" sales="2477628.23"/>



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          <nameDesignatedIndex>Global Growth Allocation Index (80% ACWI / 20% GlobalAgg)</nameDesignatedIndex>
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        <name>Janus Investment Fund</name>
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        <curCd>USD</curCd>
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        <isRestrictedSec>N</isRestrictedSec>

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        <name>iShares Trust</name>
        <lei>5493000860OXIC4B5K91</lei>
        <title>iShares Trust RUSSELL 2000 VALUE ETF</title>
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          <isin value="US4642876308"/>
          <ticker value="IWN"/>
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        <payoffProfile>Long</payoffProfile>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
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      <invstOrSec>
        <name>Ishares Inc</name>
        <lei>549300MGJZCNMJLBAJ67</lei>
        <title>Ishares Inc CORE MSCI EMERGING MKTS ETF</title>
        <cusip>46434G103</cusip>
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          <isin value="US46434G1031"/>
          <ticker value="IEMG"/>
          <other otherDesc="SEDOL" value="B8NDCB6"/>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      <invstOrSec>
        <name>iShares Trust</name>
        <lei>5493000860OXIC4B5K91</lei>
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          <isin value="US46435G6724"/>
          <ticker value="IAGG"/>
          <other otherDesc="SEDOL" value="BYQKX57"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Detroit Street Trust</name>
        <lei>5493003MM7OCK5UJW595</lei>
        <title>Janus Detroit Street Trust HENDERSON EMERGING MARKETS DEBT HARD CURRENCY ETF</title>
        <cusip>47103U738</cusip>
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          <isin value="US47103U7384"/>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Vanguard Index Funds</name>
        <lei>549300G6KNDK44WUN559</lei>
        <title>Vanguard Index Funds VANGUARD VALUE ETF</title>
        <cusip>922908744</cusip>
        <identifiers>
          <isin value="US9229087443"/>
          <ticker value="VTV"/>
          <other otherDesc="SEDOL" value="2353638"/>
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        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8907283.8</valUSD>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Janus Investment Fund</name>
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        <title>Janus Investment Fund HENDERSON CL N</title>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Janus Investment Fund</name>
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          <other otherDesc="Internal ID" value="1440"/>
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        <assetCat>EC</assetCat>
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        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
        <title>Janus Investment Fund EURPN FCS FD N</title>
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          <ticker value="HFERX"/>
          <other otherDesc="Internal ID" value="1447"/>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Investment Fund</name>
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          <isin value="US47103D8276"/>
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        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>20735932.55</valUSD>
        <pctVal>8.4203769663</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
        <title>Janus Investment Fund FLEXIBLE N</title>
        <cusip>47103D504</cusip>
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          <other otherDesc="SEDOL" value="B796JF5"/>
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        <balance>1520956.5092</balance>
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        <valUSD>14129685.97</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
        <title>Janus Investment Fund OVERSEAS N</title>
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          <other otherDesc="SEDOL" value="B8G4681"/>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
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          <isin value="US47103E1221"/>
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          <other otherDesc="SEDOL" value="2CNTNK7"/>
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        <pctVal>6.9351339423</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
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        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      </invstOrSec>
      <invstOrSec>
        <name>Janus Investment Fund</name>
        <lei>W6UYDZFUOFJ4HQQ32382</lei>
        <title>Janus Investment Fund ENTRPRS FUND N</title>
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          <other otherDesc="SEDOL" value="B8F0PT4"/>
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        <balance>152117.7736</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>20646945.41</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="895"/>
        </identifiers>
        <balance>4402139.458607</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>4402139.46</valUSD>
        <pctVal>1.7876058201</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-05-15</ncom:dateSigned>
      <ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant>
      <ncom:signature>JESPER NERGAARD</ncom:signature>
      <ncom:signerName>JESPER NERGAARD</ncom:signerName>
      <ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
