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      <regLei>W6UYDZFUOFJ4HQQ32382</regLei>
      <regStreet1>151 Detroit Street</regStreet1>
      <regCity>Denver</regCity>
      <regStateConditional regCountry="US" regState="US-CO"/>
      <regZipOrPostalCode>80206</regZipOrPostalCode>
      <regPhone>303-333-3863</regPhone>
      <seriesName>Janus Henderson European Focus Fund</seriesName>
      <seriesId>S000057603</seriesId>
      <seriesLei>549300B6VJPM3NFA9J78</seriesLei>
      <repPdEnd>2026-09-30</repPdEnd>
      <repPdDate>2026-03-31</repPdDate>
      <isFinalFiling>N</isFinalFiling>
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      <totLiabs>20613844.440000000000</totLiabs>
      <netAssets>664741188.090000000000</netAssets>
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      <assetsInvested>0.000000000000</assetsInvested>
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        <invCountry>DE</invCountry>

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        <name>Epiroc AB</name>
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        <name>ABN AMRO Bank N.V.</name>
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        <name>Siemens AG</name>
        <lei>W38RGI023J3WT1HWRP32</lei>
        <title>Siemens AG NPV(REGD)</title>
        <cusip>D69671218</cusip>
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        <invCountry>DE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

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        <name>Siemens Energy AG</name>
        <lei>5299005CHJZ14D4FDJ62</lei>
        <title>Siemens Energy AG NPV</title>
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        <assetCat>EC</assetCat>
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        <invCountry>DE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Sunbelt Rentals Holdings Inc.</name>
        <lei>529900W4BQDNMAOSRA49</lei>
        <title>Sunbelt Rentals Holdings Inc. SHS</title>
        <cusip>866966104</cusip>
        <identifiers>
          <isin value="US8669661048"/>
          <ticker value="SUNB"/>
          <other otherDesc="SEDOL" value="BW5TBS0"/>
        </identifiers>
        <balance>92758</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>5922471.18</valUSD>
        <pctVal>0.8909439171</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Orange SA</name>
        <lei>969500MCOONR8990S771</lei>
        <title>Orange SA EUR4</title>
        <cusip>F6866T100</cusip>
        <identifiers>
          <isin value="FR0000133308"/>
          <ticker value="FNCTF"/>
          <other otherDesc="SEDOL" value="5176177"/>
        </identifiers>
        <balance>769259</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>15715598.65</valUSD>
        <pctVal>2.3641680298</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>FR</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Rheinmetall AG</name>
        <lei>5299001OU9CSE29O6S05</lei>
        <title>Rheinmetall AG NPV</title>
        <cusip>D65111102</cusip>
        <identifiers>
          <isin value="DE0007030009"/>
          <ticker value="RNMBF"/>
          <other otherDesc="SEDOL" value="5334588"/>
        </identifiers>
        <balance>7968</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>13286469.98</valUSD>
        <pctVal>1.9987433031</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>DE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Nokia Corp</name>
        <lei>549300A0JPRWG1KI7U06</lei>
        <title>Nokia Corp NPV</title>
        <cusip>X61873133</cusip>
        <identifiers>
          <isin value="FI0009000681"/>
          <ticker value="NOKBF"/>
          <other otherDesc="SEDOL" value="5902941"/>
        </identifiers>
        <balance>1523048</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>12136970.32</valUSD>
        <pctVal>1.8258189108</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>FI</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Novartis AG</name>
        <lei>5493007HIVTX6SY6XD66</lei>
        <title>Novartis AG CHF0.49 (REGD)</title>
        <cusip>H5820Q150</cusip>
        <identifiers>
          <isin value="CH0012005267"/>
          <ticker value="NVSEF"/>
          <other otherDesc="SEDOL" value="7103065"/>
        </identifiers>
        <balance>187060</balance>
        <units>NS</units>
        <currencyConditional curCd="CHF" exchangeRt="0.7994"/>
        <valUSD>28408340.08</valUSD>
        <pctVal>4.2735940828</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CH</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="895"/>
        </identifiers>
        <balance>64694053.1272</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>64694053.13</valUSD>
        <pctVal>9.7322167317</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Leonardo S.p.A.</name>
        <lei>529900X4EEX1U9LN3U39</lei>
        <title>Leonardo S.p.A. NPV</title>
        <cusip>T6S996112</cusip>
        <identifiers>
          <isin value="IT0003856405"/>
          <ticker value="FINMF"/>
          <other otherDesc="SEDOL" value="B0DJNG0"/>
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        <balance>180379</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>12114083.61</valUSD>
        <pctVal>1.8223759603</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>IT</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Iberdrola, S.A.</name>
        <lei>5QK37QC7NWOJ8D7WVQ45</lei>
        <title>Iberdrola, S.A. EUR0.75</title>
        <cusip>E6165F166</cusip>
        <identifiers>
          <isin value="ES0144580Y14"/>
          <ticker value="IBDSF"/>
          <other otherDesc="SEDOL" value="B288C92"/>
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        <balance>943765</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>21661907.48</valUSD>
        <pctVal>3.2586979516</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>ES</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <loanByFundCondition isLoanByFund="Y" loanVal="229784.900000000000"/>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Knorr-Bremse AG</name>
        <lei>5299001GRRO0Z25YZT52</lei>
        <title>Knorr-Bremse AG NPV</title>
        <cusip>D4S43E114</cusip>
        <identifiers>
          <isin value="DE000KBX1006"/>
          <ticker value="KNBHF"/>
          <other otherDesc="SEDOL" value="BD2P9X9"/>
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        <balance>122003</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>13619371.11</valUSD>
        <pctVal>2.0488231140</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>DE</invCountry>

        <isRestrictedSec>Y</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Sandvik AB</name>
        <lei>5299008ZUAXN43LVZF54</lei>
        <title>Sandvik AB NPV</title>
        <cusip>W74857165</cusip>
        <identifiers>
          <isin value="SE0000667891"/>
          <ticker value="SDVKF"/>
          <other otherDesc="SEDOL" value="B1VQ252"/>
        </identifiers>
        <balance>400720</balance>
        <units>NS</units>
        <currencyConditional curCd="SEK" exchangeRt="9.4615"/>
        <valUSD>15321960.86</valUSD>
        <pctVal>2.3049513306</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>SE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Rolls-Royce Holdings PLC</name>
        <lei>213800EC7997ZBLZJH69</lei>
        <title>Rolls-Royce Holdings PLC ORD GBP0.20</title>
        <cusip>G76225104</cusip>
        <identifiers>
          <isin value="GB00B63H8491"/>
          <ticker value="RYCEF"/>
          <other otherDesc="SEDOL" value="B63H849"/>
        </identifiers>
        <balance>949259</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>14493884.03</valUSD>
        <pctVal>2.1803800170</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>NatWest Group PLC</name>
        <lei>2138005O9XJIJN4JPN90</lei>
        <title>NatWest Group PLC ORD GBP1.0769</title>
        <cusip>G6422B147</cusip>
        <identifiers>
          <isin value="GB00BM8PJY71"/>
          <ticker value="RBSPF"/>
          <other otherDesc="SEDOL" value="BM8PJY7"/>
        </identifiers>
        <balance>1258093</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>9333698.52</valUSD>
        <pctVal>1.4041101540</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Investor AB</name>
        <lei>549300VEBQPHRZBKUX38</lei>
        <title>Investor AB SER'B'NPV</title>
        <cusip>W5R777115</cusip>
        <identifiers>
          <isin value="SE0015811963"/>
          <ticker value="IVSBF"/>
          <other otherDesc="SEDOL" value="BMV7PQ4"/>
        </identifiers>
        <balance>303150</balance>
        <units>NS</units>
        <currencyConditional curCd="SEK" exchangeRt="9.4615"/>
        <valUSD>11481895.22</valUSD>
        <pctVal>1.7272730238</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>SE</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Industria De Diseno Textil SA</name>
        <lei>549300TTCXZOGZM2EY83</lei>
        <title>Industria De Diseno Textil SA EUR0.03 (POST SUBD)</title>
        <cusip>E6282J125</cusip>
        <identifiers>
          <isin value="ES0148396007"/>
          <ticker value="IDEXF"/>
          <other otherDesc="SEDOL" value="BP9DL90"/>
        </identifiers>
        <balance>140596</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>8082625.47</valUSD>
        <pctVal>1.2159056208</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>ES</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>NXP Semiconductors NV</name>
        <lei>724500M9BY5293JDF951</lei>
        <title>NXP Semiconductors NV EUR0.20</title>
        <cusip>N6596X109</cusip>
        <identifiers>
          <isin value="NL0009538784"/>
          <ticker value="NXPI"/>
          <other otherDesc="SEDOL" value="B505PN7"/>
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        <balance>41883</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8245087.38</valUSD>
        <pctVal>1.2403454950</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Shell PLC</name>
        <lei>21380068P1DRHMJ8KU70</lei>
        <title>Shell PLC ORD EUR0.07</title>
        <cusip>G80827101</cusip>
        <identifiers>
          <isin value="GB00BP6MXD84"/>
          <ticker value="RYDAF"/>
          <other otherDesc="SEDOL" value="BP6MXD8"/>
        </identifiers>
        <balance>426005</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>19890053.99</valUSD>
        <pctVal>2.9921500798</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Roche Holding AG</name>
        <lei>549300U41AUUVOAAOB37</lei>
        <title>Roche Holding AG CHF0.001</title>
        <cusip>H69293266</cusip>
        <identifiers>
          <isin value="CH1499059983"/>
          <ticker value="RHHVF"/>
          <other otherDesc="SEDOL" value="BTMJD19"/>
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        <balance>50592</balance>
        <units>NS</units>
        <currencyConditional curCd="CHF" exchangeRt="0.7994"/>
        <valUSD>19982051.09</valUSD>
        <pctVal>3.0059896164</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EP</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CH</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>TUI AG</name>
        <lei>529900SL2WSPV293B552</lei>
        <title>TUI AG ORD REG SHS NPV</title>
        <cusip>D8484K455</cusip>
        <identifiers>
          <isin value="DE000TUAG505"/>
          <ticker value="TUIFF"/>
          <other otherDesc="SEDOL" value="BNC3GJ8"/>
        </identifiers>
        <balance>407798</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>3092338.45</valUSD>
        <pctVal>0.4651943501</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>DE</invCountry>

        <isRestrictedSec>Y</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Erste Group Bank AG</name>
        <lei>PQOH26KWDF7CG10L6792</lei>
        <title>Erste Group Bank AG NPV</title>
        <cusip>A19494102</cusip>
        <identifiers>
          <isin value="AT0000652011"/>
          <ticker value="EBKOF"/>
          <other otherDesc="SEDOL" value="5289837"/>
        </identifiers>
        <balance>226641</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>24486583.41</valUSD>
        <pctVal>3.6836266277</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>AT</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Huber &amp; Suhner AG</name>
        <lei>5067007PRZ9OSX7A6787</lei>
        <title>Huber &amp; Suhner AG CHF0.25 (REGD)</title>
        <cusip>H44229187</cusip>
        <identifiers>
          <isin value="CH0030380734"/>
          <ticker value="HUBN"/>
          <other otherDesc="SEDOL" value="7132832"/>
        </identifiers>
        <balance>25269</balance>
        <units>NS</units>
        <currencyConditional curCd="CHF" exchangeRt="0.7994"/>
        <valUSD>5598461.27</valUSD>
        <pctVal>0.8422016524</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CH</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>InterContinental Hotels Group PLC</name>
        <lei>2138007ZFQYRUSLU3J98</lei>
        <title>InterContinental Hotels Group PLC ORD GBP0.208521303</title>
        <cusip>G4804L163</cusip>
        <identifiers>
          <isin value="GB00BHJYC057"/>
          <ticker value="ICHGF"/>
          <other otherDesc="SEDOL" value="BHJYC05"/>
        </identifiers>
        <balance>56809</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>7481854.01</valUSD>
        <pctVal>1.1255288741</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Finecobank Banca Fineco S.p.A.</name>
        <lei>549300L7YCATGO57ZE10</lei>
        <title>Finecobank Banca Fineco S.p.A. EUR0.33</title>
        <cusip>T4R999104</cusip>
        <identifiers>
          <isin value="IT0000072170"/>
          <ticker value="FCBBF"/>
          <other otherDesc="SEDOL" value="BNGN9Z1"/>
        </identifiers>
        <balance>418879</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>9299104.96</valUSD>
        <pctVal>1.3989060896</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>IT</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Thales SA</name>
        <lei>529900FNDVTQJOVVPZ19</lei>
        <title>Thales SA EUR3</title>
        <cusip>F9156M108</cusip>
        <identifiers>
          <isin value="FR0000121329"/>
          <ticker value="THLEF"/>
          <other otherDesc="SEDOL" value="4162791"/>
        </identifiers>
        <balance>34093</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>10008612.32</valUSD>
        <pctVal>1.5056404657</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>FR</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Wartsila Oyj Abp</name>
        <lei>743700G7A9J1PHM3X223</lei>
        <title>Wartsila Oyj Abp NPV</title>
        <cusip>X98155116</cusip>
        <identifiers>
          <isin value="FI0009003727"/>
          <ticker value="WRTBF"/>
          <other otherDesc="SEDOL" value="4525189"/>
        </identifiers>
        <balance>345891</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>12931713.35</valUSD>
        <pctVal>1.9453756713</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>FI</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>UPM-Kymmene Corporation</name>
        <lei>213800EC6PW5VU4J9U64</lei>
        <title>UPM-Kymmene Corporation NPV</title>
        <cusip>X9518S108</cusip>
        <identifiers>
          <isin value="FI0009005987"/>
          <ticker value="UPMKF"/>
          <other otherDesc="SEDOL" value="5051252"/>
        </identifiers>
        <balance>310023</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>9642087.5</valUSD>
        <pctVal>1.4505024922</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>FI</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>TotalEnergies SE</name>
        <lei>529900S21EQ1BO4ESM68</lei>
        <title>TotalEnergies SE EUR2.5</title>
        <cusip>F92124100</cusip>
        <identifiers>
          <isin value="FR0000120271"/>
          <ticker value="TTE"/>
          <other otherDesc="SEDOL" value="B15C557"/>
        </identifiers>
        <balance>257914</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>23810848.3</valUSD>
        <pctVal>3.5819727627</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>FR</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vestas Wind Systems AS</name>
        <lei>549300DYMC8BGZZC8844</lei>
        <title>Vestas Wind Systems AS DKK0.20</title>
        <cusip>K9773J201</cusip>
        <identifiers>
          <isin value="DK0061539921"/>
          <ticker value="VWSYF"/>
          <other otherDesc="SEDOL" value="BN4MYF5"/>
        </identifiers>
        <balance>90468</balance>
        <units>NS</units>
        <currencyConditional curCd="DKK" exchangeRt="6.4643"/>
        <valUSD>2709034.95</valUSD>
        <pctVal>0.4075322845</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>DK</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Technip Energies N V</name>
        <lei>724500FLODI49NSCIP70</lei>
        <title>Technip Energies N V EUR0.01</title>
        <cusip>N8486R101</cusip>
        <identifiers>
          <isin value="NL0014559478"/>
          <ticker value="THNPF"/>
          <other otherDesc="SEDOL" value="BNC0116"/>
        </identifiers>
        <balance>230378</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>9681669.41</valUSD>
        <pctVal>1.4564569766</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>FR</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>UniCredit S.p.A.</name>
        <lei>549300TRUWO2CD2G5692</lei>
        <title>UniCredit S.p.A. NPV</title>
        <cusip>T9T23L642</cusip>
        <identifiers>
          <isin value="IT0005239360"/>
          <ticker value="UNCFF"/>
          <other otherDesc="SEDOL" value="BYMXPS7"/>
        </identifiers>
        <balance>150789</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>10864014.77</valUSD>
        <pctVal>1.6343224949</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>IT</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>Royal Bank of Canada</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="RBC SL TD"/>
        </identifiers>
        <balance>48274</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>48274</valUSD>
        <pctVal>0.0072620745</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2026-04-01</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>3.65</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <cashCollateralCondition cashCollateralVal="48274.000000000000" isCashCollateral="Y"/>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Collateral Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Collateral Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="896"/>
        </identifiers>
        <balance>193097</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>193097</valUSD>
        <pctVal>0.0290484482</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <cashCollateralCondition cashCollateralVal="193097.000000000000" isCashCollateral="Y"/>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Euro Member Countries Cash Balance</name>
        <lei>N/A</lei>
        <title>Euro Member Countries Cash Balance</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="EUR Cash"/>
        </identifiers>
        <balance>-18863.78</balance>
        <units>OU</units>
        <descOthUnits>Currency</descOthUnits>
        <currencyConditional curCd="EUR" exchangeRt="0.86528"/>
        <valUSD>-18863.78</valUSD>
        <pctVal>-0.0028377631</pctVal>
        <payoffProfile>Short</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Foreign Currencies" issuerCat="OTHER"/>
        <invCountry>XX</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>United Kingdom Pound Cash Balance</name>
        <lei>N/A</lei>
        <title>United Kingdom Pound Cash Balance</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="GBP Cash"/>
        </identifiers>
        <balance>1930.78</balance>
        <units>OU</units>
        <descOthUnits>Currency</descOthUnits>
        <currencyConditional curCd="GBP" exchangeRt="0.75563"/>
        <valUSD>1930.78</valUSD>
        <pctVal>0.0002904559</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Foreign Currencies" issuerCat="OTHER"/>
        <invCountry>GB</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-05-15</ncom:dateSigned>
      <ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant>
      <ncom:signature>JESPER NERGAARD</ncom:signature>
      <ncom:signerName>JESPER NERGAARD</ncom:signerName>
      <ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
