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        </identifiers>
        <balance>487194</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>193903212</valUSD>
        <pctVal>0.9438054165</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Argenx SE</name>
        <lei>7245009C5FZE6G9ODQ71</lei>
        <title>Argenx SE SPON ADR EACH REP 1 ORD SHS</title>
        <cusip>04016X101</cusip>
        <identifiers>
          <isin value="US04016X1019"/>
          <ticker value="ARGX"/>
          <other otherDesc="SEDOL" value="BDVLM39"/>
        </identifiers>
        <balance>537873</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>392781758.25</valUSD>
        <pctVal>1.9118277986</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="GDR"/>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Blackstone Inc</name>
        <lei>5299004LW4QWGZUB8Y96</lei>
        <title>Blackstone Inc COM USD0.00001 CLASS A</title>
        <cusip>09260D107</cusip>
        <identifiers>
          <isin value="US09260D1072"/>
          <ticker value="BX"/>
          <other otherDesc="SEDOL" value="BKF2SL7"/>
        </identifiers>
        <balance>1831449</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>210598320.51</valUSD>
        <pctVal>1.0250672671</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Boeing Co.</name>
        <lei>RVHJWBXLJ1RFUBSY1F30</lei>
        <title>Boeing Co. COM USD5.00</title>
        <cusip>097023105</cusip>
        <identifiers>
          <isin value="US0970231058"/>
          <ticker value="BA"/>
          <other otherDesc="SEDOL" value="2108601"/>
        </identifiers>
        <balance>1951587</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>388424360.61</valUSD>
        <pctVal>1.8906185806</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Booking Holdings Inc</name>
        <lei>FXM8FAOHMYDIPD38UZ17</lei>
        <title>Booking Holdings Inc COM USD0.008</title>
        <cusip>09857L108</cusip>
        <identifiers>
          <isin value="US09857L1089"/>
          <ticker value="BKNG"/>
          <other otherDesc="SEDOL" value="BDRXDB4"/>
        </identifiers>
        <balance>80776</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>340092808.32</valUSD>
        <pctVal>1.6553693531</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Broadcom Inc</name>
        <lei>549300WV6GIDOZJTV909</lei>
        <title>Broadcom Inc COM USD0.001</title>
        <cusip>11135F101</cusip>
        <identifiers>
          <isin value="US11135F1012"/>
          <ticker value="AVGO"/>
          <other otherDesc="SEDOL" value="BDZ78H9"/>
        </identifiers>
        <balance>3731568</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1154957611.68</valUSD>
        <pctVal>5.6216461734</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Chipotle Mexican Grill Inc</name>
        <lei>529900REP5VGTPCP1J71</lei>
        <title>Chipotle Mexican Grill Inc COM USD0.01</title>
        <cusip>169656105</cusip>
        <identifiers>
          <isin value="US1696561059"/>
          <ticker value="CMG"/>
          <other otherDesc="SEDOL" value="B0X7DZ3"/>
        </identifiers>
        <balance>5872029</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>187963648.29</valUSD>
        <pctVal>0.9148951559</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Danaher Corporation</name>
        <lei>S4BKK9OTCEWQ3YHPFM11</lei>
        <title>Danaher Corporation COM USD0.01</title>
        <cusip>235851102</cusip>
        <identifiers>
          <isin value="US2358511028"/>
          <ticker value="DHR"/>
          <other otherDesc="SEDOL" value="2250870"/>
        </identifiers>
        <balance>1977089</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>374856074.4</valUSD>
        <pctVal>1.8245762398</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Datadog Inc</name>
        <lei>549300F6JNO0KRPO1K63</lei>
        <title>Datadog Inc COM USD0.00001 CL A</title>
        <cusip>23804L103</cusip>
        <identifiers>
          <isin value="US23804L1035"/>
          <ticker value="DDOG"/>
          <other otherDesc="SEDOL" value="BKT9Y49"/>
        </identifiers>
        <balance>3501109</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>413305917.45</valUSD>
        <pctVal>2.0117271888</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>DoorDash Inc</name>
        <lei>549300NUQ43FGGSK5051</lei>
        <title>DoorDash Inc CL A</title>
        <cusip>25809K105</cusip>
        <identifiers>
          <isin value="US25809K1051"/>
          <ticker value="DASH"/>
          <other otherDesc="SEDOL" value="BN13P03"/>
        </identifiers>
        <balance>1860978</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>279425846.7</valUSD>
        <pctVal>1.3600786955</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>DraftKings Inc.</name>
        <lei>2549006G33IUL6IVOD83</lei>
        <title>DraftKings Inc. COM CL A</title>
        <cusip>26142V105</cusip>
        <identifiers>
          <isin value="US26142V1052"/>
          <ticker value="DKNG"/>
          <other otherDesc="SEDOL" value="BLDDH12"/>
        </identifiers>
        <balance>21074132</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>455622733.84</valUSD>
        <pctVal>2.2177002622</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>GE Vernova Inc.</name>
        <lei>254900DP080RU6OK2553</lei>
        <title>GE Vernova Inc. COM</title>
        <cusip>36828A101</cusip>
        <identifiers>
          <isin value="US36828A1016"/>
          <ticker value="GEV"/>
          <other otherDesc="SEDOL" value="BP6H4Y1"/>
        </identifiers>
        <balance>519217</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>453224519.3</valUSD>
        <pctVal>2.2060271813</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Howmet Aerospace Inc</name>
        <lei>549300HO5WFZUT5N2T22</lei>
        <title>Howmet Aerospace Inc COM</title>
        <cusip>443201108</cusip>
        <identifiers>
          <isin value="US4432011082"/>
          <ticker value="HWM"/>
          <other otherDesc="SEDOL" value="BKLJ8V2"/>
        </identifiers>
        <balance>3169674</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>730483070.04</valUSD>
        <pctVal>3.5555567701</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Intercontinental Exchange Inc</name>
        <lei>5493000F4ZO33MV32P92</lei>
        <title>Intercontinental Exchange Inc COM USD0.01</title>
        <cusip>45866F104</cusip>
        <identifiers>
          <isin value="US45866F1049"/>
          <ticker value="ICE"/>
          <other otherDesc="SEDOL" value="BFSSDS9"/>
        </identifiers>
        <balance>1656709</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>260567191.52</valUSD>
        <pctVal>1.2682859876</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Eli Lilly &amp; Co.</name>
        <lei>FRDRIPF3EKNDJ2CQJL29</lei>
        <title>Eli Lilly &amp; Co. COM NPV</title>
        <cusip>532457108</cusip>
        <identifiers>
          <isin value="US5324571083"/>
          <ticker value="LLY"/>
          <other otherDesc="SEDOL" value="2516152"/>
        </identifiers>
        <balance>809542</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>744592445.34</valUSD>
        <pctVal>3.6242328106</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Internal ID" value="895"/>
        </identifiers>
        <balance>38481016.2649</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>38481016.26</valUSD>
        <pctVal>0.1873026816</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>PF</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Eaton Corporation PLC</name>
        <lei>549300VDIGTMXUNT7H71</lei>
        <title>Eaton Corporation PLC COM USD0.01</title>
        <cusip>G29183103</cusip>
        <identifiers>
          <isin value="IE00B8KQN827"/>
          <ticker value="ETN"/>
          <other otherDesc="SEDOL" value="B8KQN82"/>
        </identifiers>
        <balance>2128251</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>761211535.17</valUSD>
        <pctVal>3.7051246475</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-05-15</ncom:dateSigned>
      <ncom:nameOfApplicant>JANUS INVESTMENT FUND</ncom:nameOfApplicant>
      <ncom:signature>JESPER NERGAARD</ncom:signature>
      <ncom:signerName>JESPER NERGAARD</ncom:signerName>
      <ncom:title>VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
