<?xml version="1.0" encoding="UTF-8"?>
<edgarSubmission xmlns="http://www.sec.gov/edgar/twentyfourf2filer" xmlns:com="http://www.sec.gov/edgar/common">
  <schemaVersion>X0101</schemaVersion>
  <headerData>
    <submissionType>24F-2NT</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <filer>
        <issuerCredentials>
          <cik>0000277751</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>
      <flags>
        <overrideInternetFlag>false</overrideInternetFlag>
        <confirmingCopyFlag>false</confirmingCopyFlag>
      </flags>
      <investmentCompanyType>N-1A</investmentCompanyType>
    </filerInfo>
  </headerData>
  <formData>
    <annualFilings>
      <annualFilingInfo>
        <item1>
          <nameOfIssuer>JANUS INVESTMENT FUND</nameOfIssuer>
          <addressOfIssuer>
            <street1>151 Detroit Street</street1>
            <city>Denver</city>
            <state>CO</state>
            <country>X1</country>
            <zipCode>80206-4805</zipCode>
          </addressOfIssuer>
        </item1>
        <item2>
          <reportSeriesClass>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Balanced Fund</seriesName>
              <seriesId>S000010464</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Global Technology and Innovation Fund</seriesName>
              <seriesId>S000010465</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Growth and Income Fund</seriesName>
              <seriesId>S000010467</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Research Fund</seriesName>
              <seriesId>S000010470</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Global Select Fund</seriesName>
              <seriesId>S000010474</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Contrarian Fund</seriesName>
              <seriesId>S000010475</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Overseas Fund</seriesName>
              <seriesId>S000010476</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Triton Fund</seriesName>
              <seriesId>S000010485</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Venture Fund</seriesName>
              <seriesId>S000010488</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Global Research Fund</seriesName>
              <seriesId>S000010489</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Enterprise Fund</seriesName>
              <seriesId>S000010490</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Global Life Sciences Fund</seriesName>
              <seriesId>S000010494</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Global Real Estate Fund</seriesName>
              <seriesId>S000025889</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Forty Fund</seriesName>
              <seriesId>S000025893</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Asia Equity Fund</seriesName>
              <seriesId>S000033204</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Emerging Markets Fund</seriesName>
              <seriesId>S000057600</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson International Opportunities Fund</seriesName>
              <seriesId>S000057601</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Global Equity Income Fund</seriesName>
              <seriesId>S000057602</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson European Focus Fund</seriesName>
              <seriesId>S000057603</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
            <rptSeriesClassInfo>
              <seriesName>Janus Henderson Global Sustainable Equity Fund</seriesName>
              <seriesId>S000068819</seriesId>
              <includeAllClassesFlag>true</includeAllClassesFlag>
            </rptSeriesClassInfo>
          </reportSeriesClass>
        </item2>
        <item3>
          <investmentCompActFileNo>811-01879</investmentCompActFileNo>
          <securitiesActFileNumbers>
            <securitiesActFileNo>
              <fileNumber>002-34393</fileNumber>
            </securitiesActFileNo>
          </securitiesActFileNumbers>
        </item3>
        <item4>
          <lastDayOfFiscalYear>09/30/2022</lastDayOfFiscalYear>
          <isThisFormBeingFiledLate>false</isThisFormBeingFiledLate>
        </item4>
        <item5>
          <aggregateSalePriceOfSecuritiesSold>33514332805.00</aggregateSalePriceOfSecuritiesSold>
          <aggregatePriceOfSecuritiesRedeemedOrRepurchasedInFiscalYear>27933875589.00</aggregatePriceOfSecuritiesRedeemedOrRepurchasedInFiscalYear>
          <aggregatePriceOfSecuritiesRedeemedOrRepurchasedAnyPrior>18647155348.00</aggregatePriceOfSecuritiesRedeemedOrRepurchasedAnyPrior>
          <totalAvailableRedemptionCredits>46581030937.00</totalAvailableRedemptionCredits>
          <netSales>0.00</netSales>
          <redemptionCreditsAvailableForUseInFutureYears>(13066698132.00)</redemptionCreditsAvailableForUseInFutureYears>
          <multiplierForDeterminingRegistrationFee>0.0001102</multiplierForDeterminingRegistrationFee>
          <registrationFeeDue>0.00</registrationFeeDue>
        </item5>
        <item6>
          <amountOfSecuritiesDeducted>0.00</amountOfSecuritiesDeducted>
          <numberOfSharesOrOtherUnitsRemainingUnsold>0.00</numberOfSharesOrOtherUnitsRemainingUnsold>
        </item6>
        <item7>
          <interestDue>0.00</interestDue>
        </item7>
        <item8>
          <totalOfRegistrationFeePlusAnyInterestDue>0.00</totalOfRegistrationFeePlusAnyInterestDue>
        </item8>
        <signature>
          <signature>Wendy Christiansen</signature>
          <nameAndTitle>Senior Fund Oversight Analyst</nameAndTitle>
          <signatureDate>12/29/2022</signatureDate>
        </signature>
      </annualFilingInfo>
    </annualFilings>
  </formData>
</edgarSubmission>
