N-MFP2 0000277751 XXXXXXXX 2022-03-31 0000277751 W6UYDZFUOFJ4HQQ32382 S000010466 2 N N 002-34393 JANUS HENDERSON INVESTORS US LLC 801-13991 PRICEWATERHOUSECOOPERS LLP DENVER CO JANUS HENDERSON INVESTORS US LLC JANUS HENDERSON SERVICES US LLC 0001170078 084-06024 N N N Exempt Government Government/Agency N 21 21 161614764.71 162020129.71 163446663.10 151934528.57 333385557.94 348786694.19 337712255.37 346192045.39 .4387 .4277 .4327 .4039 .9050 .9208 .8941 .9203 129055.54 377899549.45 377919247.89 22647.17 5094614.23 372976336.37 372974211.86 1.0000 .0028 1.0000 1.0000 1.0000 1.0000 C000028921 2500.00 7021175.42 7021183.19 1.0000 1.0000 1.0000 1.0000 390152.23 60575.24 155735.11 253262.14 98409.68 98973.31 219125.22 193437.66 862938.00 910688.61 .0000 Y Janus Henderson Investors US LLC? voluntarily waived/reimbursed fees of $2,431.49 to assist the Fund in attempting to maintain a yield of at least 0.00%. C000083514 2500.00 365955160.95 365953028.67 1.0000 1.0000 1.0000 1.0000 5200225.81 3271975.57 12811621.57 2317384.09 3572915.15 4667616.96 3312435.87 4864083.02 27073837.75 18995186.72 .0000 Y Janus Henderson Investors US LLC? voluntarily waived/reimbursed fees of $115,775.13 to assist the Fund in attempting to maintain a yield of at least 0.00%. Bryan W Kelley 2019 Irrevocable Insurance Trust Bryan W Kelley 2019 Irrevocable Insurance Trust 0.540000% 117626AA9 US117626AA95 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2070-07-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0054 2980000.00 2980000.00 .0080 N N Y N Cash Management Bill Cash Management Bill 0.096379% 912796T90 254900HROIFWPRGM1V77 US912796T908 BNZHR16 U.S. Treasury Debt Y Moody's P-1 2022-04-19 2022-04-19 2022-04-19 N N N .0010 4999653.10 4999653.10 .0134 N Y Y N Cypress Bend Real Estate Development Co LLC Cypress Bend Real Estate Development Co LLC 0.600000% 23267QAB8 US23267QAB86 BYSV0V3 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2033-04-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0060 9000000.00 9000000.00 .0241 N N Y N Federal Farm Credit Discount Notes Federal Farm Credit Discount Notes 0.170158% 313313WD6 US313313WD65 U.S. Government Agency Debt Y Moody's P-1 2022-04-29 2022-04-29 2022-04-29 N N N .0017 4999339.50 4999339.50 .0134 N N Y N Federal Farm Credit Discount Notes Federal Farm Credit Discount Notes 0.203007% 313313WJ3 US313313WJ36 U.S. Government Agency Debt Y Moody's P-1 2022-05-04 2022-05-04 2022-05-04 N N N .0020 4998079.40 4998079.40 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.053150% 313385UZ7 2549001DPIFGXC1TOL40 US313385UZ72 U.S. Government Agency Debt Y Moody's P-1 2022-04-01 2022-04-01 2022-04-01 N N N .0005 5000000.00 5000000.00 .0134 N Y Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.120710% 313385VC7 2549001DPIFGXC1TOL40 US313385VC78 BH7QZJ3 U.S. Government Agency Debt Y Moody's P-1 2022-04-04 2022-04-04 2022-04-04 N N N .0012 9999858.50 9999858.50 .0268 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.091295% 313385VD5 2549001DPIFGXC1TOL40 US313385VD51 BDFY1Y8 U.S. Government Agency Debt Y Moody's P-1 2022-04-05 2022-04-05 2022-04-05 N N N .0009 4999905.65 4999905.65 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.171053% 313385VE3 2549001DPIFGXC1TOL40 US313385VE35 BDFY223 U.S. Government Agency Debt Y Moody's P-1 2022-04-06 2022-04-06 2022-04-06 N N N .0017 4999882.05 4999882.05 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.163680% 313385VG8 2549001DPIFGXC1TOL40 US313385VG82 U.S. Government Agency Debt Y Moody's P-1 2022-04-08 2022-04-08 2022-04-08 N N N .0016 9999669.70 9999669.70 .0268 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.140331% 313385VK9 2549001DPIFGXC1TOL40 US313385VK94 BJ2SGD9 U.S. Government Agency Debt Y Moody's P-1 2022-04-11 2022-04-11 2022-04-11 N N N .0014 4999764.10 4999764.10 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.121745% 313385VL7 2549001DPIFGXC1TOL40 US313385VL77 U.S. Government Agency Debt Y Moody's P-1 2022-04-12 2022-04-12 2022-04-12 N N N .0012 4999740.50 4999740.50 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.119777% 313385VM5 2549001DPIFGXC1TOL40 US313385VM50 BYVWMY0 U.S. Government Agency Debt Y Moody's P-1 2022-04-13 2022-04-13 2022-04-13 N N N .0012 4999716.90 4999716.90 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.127509% 313385VS2 2549001DPIFGXC1TOL40 US313385VS21 U.S. Government Agency Debt Y Moody's P-1 2022-04-18 2022-04-18 2022-04-18 N N N .0013 4999598.65 4999598.65 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.177250% 313385VU7 2549001DPIFGXC1TOL40 US313385VU76 B4R4R45 U.S. Government Agency Debt Y Moody's P-1 2022-04-20 2022-04-20 2022-04-20 N N N .0018 4999551.80 4999551.80 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.116616% 313385VW3 2549001DPIFGXC1TOL40 US313385VW33 U.S. Government Agency Debt Y Moody's P-1 2022-04-22 2022-04-22 2022-04-22 N N N .0012 9999009.20 9999009.20 .0268 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.188158% 313385WB8 2549001DPIFGXC1TOL40 US313385WB86 BFYV9X9 U.S. Government Agency Debt Y Moody's P-1 2022-04-27 2022-04-27 2022-04-27 N N N .0019 4999386.65 4999386.65 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.240342% 313385WG7 2549001DPIFGXC1TOL40 US313385WG73 BJVX8S4 U.S. Government Agency Debt Y Moody's P-1 2022-05-02 2022-05-02 2022-05-02 N N N .0024 4998195.80 4998195.80 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.240341% 313385WH5 2549001DPIFGXC1TOL40 US313385WH56 BDRTGW4 U.S. Government Agency Debt Y Moody's P-1 2022-05-03 2022-05-03 2022-05-03 N N N .0024 4998137.60 4998137.60 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.216916% 313385WJ1 2549001DPIFGXC1TOL40 US313385WJ13 BG0W8R0 U.S. Government Agency Debt Y Moody's P-1 2022-05-04 2022-05-04 2022-05-04 N N N .0022 4998079.40 4998079.40 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.230030% 313385WL6 2549001DPIFGXC1TOL40 US313385WL68 U.S. Government Agency Debt Y Moody's P-1 2022-05-06 2022-05-06 2022-05-06 N N N .0023 9995926.00 9995926.00 .0268 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.266204% 313385WR3 2549001DPIFGXC1TOL40 US313385WR39 BG05727 U.S. Government Agency Debt Y Moody's P-1 2022-05-11 2022-05-11 2022-05-11 N N N .0027 4997672.10 4997672.10 .0134 N N Y N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.295504% 313385WT9 2549001DPIFGXC1TOL40 US313385WT94 U.S. Government Agency Debt Y Moody's P-1 2022-05-13 2022-05-13 2022-05-13 N N N .0030 4997555.75 4997555.75 .0134 N N 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2022-06-15 2022-06-15 N N N .0047 4993565.75 4993565.75 .0134 N N N N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.446355% 313385YE0 2549001DPIFGXC1TOL40 US313385YE08 U.S. Government Agency Debt Y Moody's P-1 2022-06-17 2022-06-17 2022-06-17 N N N .0045 4993394.30 4993394.30 .0134 N N N N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.602262% 313385YS9 2549001DPIFGXC1TOL40 US313385YS93 BFZCT42 U.S. Government Agency Debt Y Moody's P-1 2022-06-29 2022-06-29 2022-06-29 N N N .0060 4992365.60 4992365.60 .0134 N N N N Federal Home Loan Bank Discount Notes Federal Home Loan Bank Discount Notes 0.649500% 313385YU4 2549001DPIFGXC1TOL40 US313385YU40 U.S. Government Agency Debt Y Moody's P-1 2022-07-01 2022-07-01 2022-07-01 N N N .0065 4990412.85 4990412.85 .0134 N N N N Goldman Sachs & Co. Joint Repurchase Agreement Goldman Sachs & Co. Joint Repurchase Agreement 0.290000% GS0401A22 U.S. Government Agency Repurchase Agreement, collateralized only by U.S. Government Agency securities, U.S. Treasuries, and cash Y N Ginnie Mae 2026-11-20 2061-08-16 0.35% to 4.50% 20342771.21 20400000.00 Agency Mortgage-Backed Securities Y Standard & Poor A-1 2022-04-01 2022-04-01 2022-04-01 N N N .0029 20000000.00 20000000.00 .0536 N Y Y N HSBC Securities (USA), Inc. Joint Repurchase Agreement HSBC Securities (USA), Inc. Joint Repurchase Agreement 0.290000% HS0401A22 U.S. Government Agency Repurchase Agreement, collateralized only by U.S. Government Agency securities, U.S. Treasuries, and cash Y N U.S. Treasury 2023-08-15 2052-02-15 0.00% to 3.00% 12539095.00 10200001.21 U.S. Treasuries (including strips) Y Standard & Poor A-1 2022-04-01 2022-04-01 2022-04-01 N N N .0029 10000000.00 10000000.00 .0268 N Y Y N ING Financial Markets LLC Joint Repurchase Agreement ING Financial Markets LLC Joint Repurchase Agreement 0.270000% IG0401A22 U.S. Government Agency Repurchase Agreement, collateralized only by U.S. Government Agency securities, U.S. Treasuries, and cash Y N U.S. Treasury 2022-04-21 2052-02-15 0.00% to 5.38% 15627675.68 15300114.79 U.S. Treasuries (including strips) Y Standard & Poor A-1 2022-04-01 2022-04-01 2022-04-01 N N N .0027 15000000.00 15000000.00 .0402 N Y Y N ING Financial Markets LLC Joint Repurchase Agreement ING Financial Markets LLC Joint Repurchase Agreement 0.280000% IG0401B22 U.S. Government Agency Repurchase Agreement, collateralized only by U.S. Government Agency securities, U.S. Treasuries, and cash Y N Freddie Mac 2029-11-01 2051-11-01 1.50% to 7.00% 4387240.94 4530801.47 Agency Mortgage-Backed Securities Fannie Mae 2028-08-01 2057-01-01 1.50% to 5.50% 13916392.56 13614420.40 Agency Mortgage-Backed Securities Ginnie Mae 2042-10-20 2051-01-20 2.50% to 4.50% 2272576.00 2254936.81 Agency Mortgage-Backed Securities Y Standard & Poor A-1 2022-04-01 2022-04-01 2022-04-01 N N N .0028 20000000.00 20000000.00 .0536 N Y Y N Irvine Inn Apartments LP Irvine Inn Apartments LP 0.550000% 46363PAA6 549300JMOWDNNRVB6C12 US46363PAA66 BKTHZ84 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2060-02-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0055 4000000.00 4000000.00 .0107 N N Y N John H Smith Irrevocable Insurance Trust of 2017/The John H Smith Irrevocable Insurance Trust of 2017/The 0.600000% 47802VAA0 US47802VAA08 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2041-02-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0060 5820000.00 5820000.00 .0156 N N Y N Johnson Capital Management LLC Johnson Capital Management LLC 0.550000% 478186AA7 US478186AA77 BYSX0M0 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2047-06-03 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0056 2845000.00 2845000.00 .0076 N N Y N LML Trust LML Trust 0.600000% 53952QAA7 US53952QAA76 BKY8B06 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2041-02-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0060 4500000.00 4500000.00 .0121 N N Y N New Sawmill Ridge LLC New Sawmill Ridge LLC 0.966900% 648638AA2 US648638AA20 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2061-06-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0097 3900000.00 3900000.00 .0105 N N Y N Olivetree Apartments LP Olivetree Apartments LP 0.550000% 681042AA5 549300RNLU3FHTUUVF66 US681042AA54 BLCVQ21 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2059-06-02 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0055 7000000.00 7000000.00 .0188 N N Y N Royal Bank of Canada, NY Branch Joint Repurchase Agreement Royal Bank of Canada, NY Branch Joint Repurchase Agreement 0.290000% RY0401A22 U.S. Government Agency Repurchase Agreement, collateralized only by U.S. Government Agency securities, U.S. Treasuries, and cash Y N Freddie Mac 2031-02-15 2041-03-15 6.02% to 31.27% 326400.02 348808.67 Agency Mortgage-Backed Securities Fannie Mae 2040-07-25 2041-04-25 8.54% to 12.58% 134991.92 134664.30 Agency Mortgage-Backed Securities Ginnie Mae 2034-09-20 2047-07-20 0.00% to 21.18% 50189.47 51293.56 Agency Mortgage-Backed Securities U.S. Treasury 2022-06-21 2042-02-15 0.00% to 0.75% 28409739.19 28739469.30 U.S. Treasuries (including strips) Y Standard & Poor A-1+ 2022-04-01 2022-04-01 2022-04-01 N N N .0029 28700000.00 28700000.00 .0769 N Y Y N Royal Bank of Canada, NY Branch Joint Repurchase Agreement Royal Bank of Canada, NY Branch Joint Repurchase Agreement 0.270000% RY0401C22 U.S. Government Agency Repurchase Agreement, collateralized only by U.S. Government Agency securities, U.S. Treasuries, and cash Y N Freddie Mac 2028-07-15 2036-04-15 5.50% to 6.50% 13311.27 14328.42 Agency Mortgage-Backed Securities Ginnie Mae 2040-12-20 2051-07-20 3.50% to 6.85% 1277912.70 1332654.19 Agency Mortgage-Backed Securities U.S. Treasury 2028-06-30 2050-08-15 1.25% to 2.38% 14825685.00 13953132.15 U.S. Treasuries (including strips) Y Standard & Poor A-1+ 2022-04-01 2022-04-01 2022-04-01 N N N .0027 15000000.00 15000000.00 .0402 N Y Y N SMZ Holdings LLC SMZ Holdings LLC 0.600000% 78460YAA2 US78460YAA29 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2040-06-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0060 4240000.00 4240000.00 .0114 N N Y N United States Treasury Bill United States Treasury Bill 0.074519% 912796G45 254900HROIFWPRGM1V77 US912796G459 BMG87D3 U.S. Treasury Debt Y Moody's P-1 2022-04-21 2022-04-21 2022-04-21 N N N .0007 4999659.70 4999659.70 .0134 N Y Y N United States Treasury Bill United States Treasury Bill 0.058577% 912796N47 254900HROIFWPRGM1V77 US912796N471 BM9XBK0 U.S. Treasury Debt Y Moody's P-1 2022-04-07 2022-04-07 2022-04-07 N N N .0006 4999879.15 4999879.15 .0134 N Y Y N United States Treasury Bill United States Treasury Bill 0.091980% 912796P45 254900HROIFWPRGM1V77 US912796P450 BNM0KC0 U.S. Treasury Debt Y Moody's P-1 2022-05-05 2022-05-05 2022-05-05 N N N .0009 4999303.45 4999303.45 .0134 N Y Y N United States Treasury Bill United States Treasury Bill 0.301210% 912796Q28 254900HROIFWPRGM1V77 US912796Q284 BMG1YH5 U.S. Treasury Debt Y Moody's P-1 2022-05-12 2022-05-12 2022-05-12 N N N .0030 9997819.00 9997819.00 .0268 N Y Y N United States Treasury Bill United States Treasury Bill 0.242617% 912796U72 254900HROIFWPRGM1V77 US912796U724 BNLZ7V4 U.S. Treasury Debt Y Moody's P-1 2022-05-03 2022-05-03 2022-05-03 N N N .0024 4999411.10 4999411.10 .0134 N Y Y N United States Treasury Bill United States Treasury Bill 0.293386% 912796U80 254900HROIFWPRGM1V77 US912796U807 BMX8CV9 U.S. Treasury Debt Y Moody's P-1 2022-05-10 2022-05-10 2022-05-10 N N N .0029 4999004.70 4999004.70 .0134 N Y Y N Ventana Housing LP Ventana Housing LP 0.550000% 922760AA1 54930063Z7PR37DWY412 US922760AA11 Variable Rate Demand Note Y N/A N/A 2022-04-07 2022-04-07 2060-06-01 Y Federal Home Loan Bank 1.0000 7 N Moody's P-1 Y Federal Home Loan Bank 1.0000 Moody's P-1 N .0055 5000000.00 5000000.00 .0134 N N Y N JANUS INVESTMENT FUND 2022-04-07 Jesper Nergaard Jesper Nergaard Vice President, Chief Financial Officer, Treasurer and Principal Accounting Officer of Janus Investment Fund