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      <repPdEnd>2025-03-31</repPdEnd>
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        <name>Msilf Government Portfolio</name>
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        <name>N/A</name>
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        <name>Treasury Bill</name>
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        <lei>N/A</lei>
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        <name>N/A</name>
        <lei>N/A</lei>
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          <other otherDesc="INTERNAL IDENTIFIER" value="P797NT05"/>
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        <valUSD>-9000000.00000000</valUSD>
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          <transCat>Reverse repurchase</transCat>
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              <counterpartyInfo lei="549300MGEH3N3CUCV828" name="UMB Financial Corporation"/>
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            <repurchaseCollateral>
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      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
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        <name>N/A</name>
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        <name>N/A</name>
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        <issuerCat>CORP</issuerCat>
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      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
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        <cusip>N/A</cusip>
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          <ticker value="NKPREF2"/>
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        <assetCat>EP</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>3</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
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        <valUSD>2250000.00000000</valUSD>
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        <assetCat>LON</assetCat>
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      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>Viron International, LLC (Common Stock)</title>
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          <ticker value="Viron EQ"/>
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        <invCountry>US</invCountry>
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          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
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          <ticker value="NKPREF1"/>
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        <curCd>USD</curCd>
        <valUSD>3008.90000000</valUSD>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>EP</assetCat>
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        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>3</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
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          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>Nielsen-Kellerman Acquisition Corp. (Line of Credit)</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="INTERNAL ID" value="KLOC-310"/>
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        <balance>30449.83000000</balance>
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        <curCd>USD</curCd>
        <valUSD>30449.83000000</valUSD>
        <pctVal>0.151282257964</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>LON</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        <fairValLevel>3</fairValLevel>
        <debtSec>
          <maturityDt>2025-12-31</maturityDt>
          <couponKind>Floating</couponKind>
          <annualizedRt>9.55270000</annualizedRt>
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          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
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        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
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      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2025-04-30</ncom:dateSigned>
      <ncom:nameOfApplicant>Gladstone Alternative Income Fund</ncom:nameOfApplicant>
      <ncom:signature>Michael J. Malesardi</ncom:signature>
      <ncom:signerName>Michael J. Malesardi</ncom:signerName>
      <ncom:title>Chief Financial Officer and Treasurer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
