<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-01-22</periodOfReport>

    <issuer>
        <issuerCik>0001901637</issuerCik>
        <issuerName>USCB FINANCIAL HOLDINGS, INC.</issuerName>
        <issuerTradingSymbol>USCB</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001999607</rptOwnerCik>
            <rptOwnerName>Logrono Maricarmen</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O USCB FINANCIAL HOLDINGS, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>2301 N.W. 87TH AVENUE</rptOwnerStreet2>
            <rptOwnerCity>DORAL</rptOwnerCity>
            <rptOwnerState>FL</rptOwnerState>
            <rptOwnerZipCode>33172</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>1</isOfficer>
            <officerTitle>EVP and Chief Risk Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Voting Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2025-01-22</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>F</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>260</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>18.27</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>10936</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Options to Purchase Class A Voting Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>12.0500</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2022-09-27</value>
                <footnoteId id="F2"/>
            </exerciseDate>
            <expirationDate>
                <value>2031-09-27</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Class A Voting Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>15000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>15000</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Includes 1,753 shares of restricted stock of which 877 shares vest on January 22, 2026 and 876 shares vest on January 22, 2027, and 1,617 shares of restricted stock which commenced vesting at a rate of one-third per year which commencing on March 8, 2024, and 5,880 shares of restricted stock which vest at a rate of one-third per year commencing on January 21, 2026.</footnote>
        <footnote id="F2">The options vest at a rate of one-third per year commencing on the first anniversary of the date of grant, which was 9/27/2021.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Robert Anderson by P.O.A. for Maricarmen Logrono</signatureName>
        <signatureDate>2025-01-24</signatureDate>
    </ownerSignature>
</ownershipDocument>
