<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2025-03-06</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001754195</issuerCik>
        <issuerName>Trulieve Cannabis Corp.</issuerName>
        <issuerTradingSymbol>TRUL</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001983133</rptOwnerCik>
            <rptOwnerName>Blust Ryan</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>6749 BEN BOSTIC ROAD</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>QUINCY</rptOwnerCity>
            <rptOwnerState>FL</rptOwnerState>
            <rptOwnerZipCode>32351</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Interim CFO</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Subordinate Voting Shares</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>54298</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>33.42</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F2"/>
            </exerciseDate>
            <expirationDate>
                <value>2026-01-04</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Subordinate Voting Shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>7497</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>26.88</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F3"/>
            </exerciseDate>
            <expirationDate>
                <value>2028-09-29</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Subordinate Voting Shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>19458</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>21.48</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F4"/>
            </exerciseDate>
            <expirationDate>
                <value>2029-02-24</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Subordinate Voting Shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>24860</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>3.99</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F5"/>
            </exerciseDate>
            <expirationDate>
                <value>2030-07-24</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Subordinate Voting Shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>56442</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>10</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F6"/>
            </exerciseDate>
            <expirationDate>
                <value>2031-03-08</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Subordinate Voting Shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>33114</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The amount of securities beneficially owned in column 2 includes two grants of restricted stock units (&quot;RSUs&quot;). Each RSU represents a contingent right to receive one Trulieve Cannabis Corp. subordinate voting share. The reporting person was granted: (i) 25,364 RSUs on July 25, 2023, subject to 2-year vesting; the RSUs vested 50% on December 1, 2024 and will vest 50% on December 1, 2025; and (ii) 18,288 RSUs on March 8, 2024, subject to 2-year vesting; the RSUs will vest 50% on December 1, 2025 and 50% on December 1, 2026.</footnote>
        <footnote id="F2">The options were granted on January 4, 2021. As of December 31, 2023, all of the options were fully vested and exercisable.</footnote>
        <footnote id="F3">The options were granted on September 29, 2021. As of December 1, 2023, all of the options were fully vested and exercisable.</footnote>
        <footnote id="F4">The options were granted on February 24, 2022. As of December 1, 2024, all of the options were fully vested and exercisable.</footnote>
        <footnote id="F5">The options were granted on July 25, 2023, and vest 33% per year in equal installments, starting on December 1 of the grant year. One third of the options vested and became exercisable on December 1, 2023; one third of the options vested and became exercisable on December 1, 2024; and one third of the options will vest and become exercisable on December 1, 2025.</footnote>
        <footnote id="F6">The options were granted on March 8, 2024, and vest 33% per year in equal installments, starting on December 1 of the grant year. One third of the options vested and became exercisable on December 1, 2024; one third of the options will vest and become exercisable on December 1, 2025; and one third of the options will vest and become exercisable on December 1, 2026.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Ryan Blust</signatureName>
        <signatureDate>2025-03-11</signatureDate>
    </ownerSignature>
</ownershipDocument>
