SCHEDULE 13G 0002005369 XXXXXXXX LIVE Common Shares 01/21/2026 0001980845 enGene Holdings Inc. 29286M105 4868 RUE LEVY, SUITE 220 SAINT-LAURENT A8 H4R 2P1 Rule 13d-1(c) Invus Public Equities, L.P. D0 3675408.00 0.00 3675408.00 0.00 3675408.00 N 5.5 PN Invus Public Equities Advisors, LLC DE 3675408.00 0.00 3675408.00 0.00 3675408.00 N 5.5 OO Invus Global Management, LLC DE 3675408.00 0.00 3675408.00 0.00 3675408.00 N 5.5 OO Siren, L.L.C. DE 3675408.00 0.00 3675408.00 0.00 3675408.00 N 5.5 OO Avicenna Life Sci Master Fund LP E9 569151.00 0.00 569151.00 0.00 569151.00 N 0.8 PN Avicenna Life Sci Master GP LLC DE 569151.00 0.00 569151.00 0.00 569151.00 N 0.8 OO Ulys, L.L.C. DE 569151.00 0.00 569151.00 0.00 569151.00 N 0.8 OO Raymond Debbane R1 4244559.00 0.00 4244559.00 0.00 4244559.00 N 6.3 IN enGene Holdings Inc. 4868 RUE LEVY, SUITE 220, SAINT-LAURENT, Quebec, Canada, H4R 2P1 See Item 2(c) below. See Item 2(c) below. (i) Invus Public Equities, L.P. ("Invus Public Equities") 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Bermuda limited partnership (ii) Invus Public Equities Advisors, LLC ("Invus PE Advisors") 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Delaware limited liability company (iii) Invus Global Management, LLC ("Global Management") 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Delaware limited liability company (iv) Siren, L.L.C. ("Siren") c/o The Invus Group, LLC, 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Delaware limited liability company (v) Avicenna Life Sci Master Fund LP ("Avicenna Fund") 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Cayman Islands limited partnership (vi) Avicenna Life Sci Master GP LLC ("Avicenna GP") 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Delaware limited liability company (vii) Ulys, L.L.C. ("Ulys") 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Delaware limited liability company (viii) Mr. Raymond Debbane 750 Lexington Avenue, 30th Floor, New York, NY 10022 Citizenship: Panama The foregoing persons are hereinafter sometimes collectively referred to as the "Reporting Persons." Y Each of the Reporting Persons may be deemed to be the beneficial owner of the Common Shares (the "Shares") listed on such Reporting Person's cover page. As of January 27, 2026, Invus Public Equities directly held 3,675,408 Shares and Avicenna Fund directly held 569,151 Shares. Invus PE Advisors, as the general partner of Invus Public Equities, controls Invus Public Equities and, accordingly, may be deemed to beneficially own the Shares directly held by Invus Public Equities. Global Management, as the managing member of Invus PE Advisors, controls Invus PE Advisors and, accordingly, may be deemed to beneficially own the Shares that Invus PE Advisors may be deemed to beneficially own. Siren, as the managing member of Global Management, controls Global Management and, accordingly, may be deemed to beneficially own the Shares that Global Management may be deemed to beneficially own. Avicenna GP, as the general partner of Avicenna Fund, controls Avicenna Fund and, accordingly, may be deemed to beneficially own the Shares directly held by Avicenna Fund. Ulys, as the managing member of Avicenna GP, controls Avicenna GP and, accordingly, may be deemed to beneficially own the Shares that Avicenna GP may be deemed to beneficially own. Mr. Raymond Debbane, as the managing member of Siren and Ulys, controls Siren and Ulys and, accordingly, may be deemed to beneficially own the Shares that Siren and Ulys may be deemed to beneficially own. As of January 27, 2026, each of the Reporting Persons may be deemed to be the beneficial owner of the percentage of Shares listed on such Reporting Person's cover page. Calculations of the percentage of Shares beneficially owned are based on 66,984,661 Shares outstanding as of December 17, 2025, based on information provided by the Issuer in its annual report on Form 10-K as filed with the Securities and Exchange Commission on December 22, 2025. See each cover page hereof. See each cover page hereof. See each cover page hereof. See each cover page hereof. Y Y Y Y Y N Each of the Reporting Persons hereby makes the following certification: By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Invus Public Equities, L.P. /s/ Raymond Debbane 01/27/2026 Invus Public Equities Advisors, LLC /s/ Raymond Debbane 01/27/2026 Invus Global Management, LLC /s/ Raymond Debbane 01/27/2026 Siren, L.L.C. /s/ Raymond Debbane 01/27/2026 Avicenna Life Sci Master Fund LP /s/ Raymond Debbane 01/27/2026 Avicenna Life Sci Master GP LLC /s/ Raymond Debbane 01/27/2026 Ulys, L.L.C. /s/ Raymond Debbane 01/27/2026 Raymond Debbane /s/ Raymond Debbane 01/27/2026