X0206 3 2025-07-08 1 0001980845 enGene Holdings Inc. ENGN 0001448914 Heffernan Michael Thomas C/O 4868 RUE LEVY, SUITE 220 SAINT-LAURENT A8 H4R 2P1 QUEBEC, CANADA true Exhibit 24 - Power of Attorney /s/ Lee Giguere, as attorney-in-fact for the Reporting Person 2025-07-08