X0206 3 2025-07-08 0 0001980845 enGene Holdings Inc. ENGN 0001780426 Buck Jill C/O 4868 RUE LEVY, SUITE 220 SAINT-LAURENT A8 H4R 2P1 QUEBEC, CANADA true Chief Development Officer Stock Option (Right to Buy) 6.6 2034-09-30 Common Shares 68000 D Stock Option (Right to Buy) 7.39 2035-01-29 Common Shares 51000 D Non-qualified stock option grant awarded as an inducement award outside of the Amended and Restated enGene Holdings Inc. 2023 Incentive Equity Plan in accordance with NASDAQ Listing Rule 5635(c)(4). This option vests at 25% on September 9, 2025, which is the first anniversary of the employment commencement date, with the remaining portion to vest monthly over the next three years, subject to the reporting person's continued service. This option vests in equal monthly installments over forty-eight (48) months on the last day of each month commencing January 29, 2025, subject to the reporting person's continued service. Exhibit 24 - Power of Attorney /s/ Lee Giguere, as attorney-in-fact for the Reporting Person 2025-07-08